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Bovine serum albumin-platinum/bismuth composite nano material mimic peroxidase

A technology of bovine serum albumin and composite nanomaterials, which is applied in the field of nanotechnology and bionics, can solve the problems of being easily disturbed by the external environment, low content of natural peroxidase, and high price, and achieve good long-term stability at room temperature and easy operation Simple, fast and stable effect

Inactive Publication Date: 2016-07-06
FUJIAN MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The content of natural peroxidase is low, it is easily disturbed by the external environment, and the price is expensive

Method used

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  • Bovine serum albumin-platinum/bismuth composite nano material mimic peroxidase
  • Bovine serum albumin-platinum/bismuth composite nano material mimic peroxidase
  • Bovine serum albumin-platinum/bismuth composite nano material mimic peroxidase

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0041] Add 5 mL of 16 mmol / L chloroplatinic acid solution and 0.5 mL of 1.5 mol / L sodium hydroxide solution to 5 mL of bovine serum albumin aqueous solution with a concentration of 50 mg / mL, mix well, and react in a water bath at 80°C for 2 h. After the reaction, the solution was put into an ultrafiltration tube with a molecular weight cut-off of 3k, centrifuged and ultrafiltered at 6000r / min, and washed 3 times with water to obtain an aqueous solution of bovine serum albumin-platinum nanocomposite material, and the aqueous solution of bovine serum albumin-platinum nanocomposite material was frozen Dry to obtain the powder of bovine serum albumin-platinum nanocomposite material. Add 0.6 mL of bismuth nitrate solution with a concentration of 0.5 mmol / L and 1.8 mL of a phosphate buffer solution with a concentration of 10 mmol / L and pH=7 in 0.6 mL of bovine serum albumin-platinum aqueous solution with a concentration of 23.8 mg / mL, and mix After homogenization, react in a water b...

example 2

[0043] Add 1 mL of hydrogen peroxide with a concentration of 2 mol / L and 0.2 mL of 3,3',5,5'-tetra Methylbenzidine hydrochloride, 10 μL of 4.76 mg / mL Example 1 prepared The bovine serum albumin-platinum / bismuth composite nanomaterial or the bovine serum albumin-platinum nanomaterial aqueous solution was mixed and incubated at 37° C. for 10 minutes, and the ultraviolet-visible absorption spectrum was measured. like figure 1 As shown, both bovine serum albumin-platinum / bismuth composite nanomaterials and bovine serum albumin-platinum nanomaterials can catalyze the color development of 3,3',5,5'-tetramethylbenzidine hydrochloride, with a According to the maximum absorption peak, the simulated peroxidase activity of bovine serum albumin-platinum / bismuth composite nanomaterials is significantly higher than that of bovine serum albumin-platinum nanomaterials.

example 3

[0045] Add 1 mL of hydrogen peroxide with a concentration of 2 mol / L, 0.2 mL of 3,3',5 ,5'-Tetramethylbenzidine hydrochloride, 10 μL of 4.76 mg / mL Example 1 prepared The bovine serum albumin-platinum / bismuth composite nanomaterial aqueous solution was mixed and incubated at 37°C for 10 minutes, and the absorbance at a wavelength of 652nm was measured. like figure 2 As shown, the simulated peroxidase activity of bovine serum albumin-platinum / bismuth composite nanomaterials reaches the maximum at pH=5.

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Abstract

The invention discloses bovine serum albumin-platinum / bismuth composite nano material mimic peroxidase. A bismuth nitrate water solution is added into a bovine serum albumin-platinum nano material water solution; after the water solution is uniformly mixed with a phosphate buffering solution, the mixed solution is heated in a water bath; and ultra-filtration and water washing are carried out on the heated mixed solution to obtain the bismuth-bovine serum albumin-platinum composite nano material mimic peroxidase. The bovine serum albumin-platinum / bismuth composite nano material mimic peroxidase can be used for catalyzing hydrogen peroxide to oxidize 3,3',5,5'-tetramethylbenzidine dihydrochloride so as to develop the color; and the Michaelis constant on the 3,3',5,5'-tetramethylbenzidine dihydrochloride is 0.054mmol / L and the Michaelis constant on the hydrogen peroxide is 13.24mmol / L. Meanwhile, the mimic peroxidase has the advantages of acid and alkali resistance, high temperature resistance and high salt resistance, and has excellent room temperature stability.

Description

technical field [0001] The invention relates to a bovine serum albumin-platinum / bismuth composite nano material with the characteristic of simulating peroxidase, and belongs to the field of nano technology and bionic technology. Background technique [0002] An enzyme is a protein, RNA or complex that has catalytic activity in an organism. The specificity, efficiency and controllability of enzyme catalysis determine that it is the core substance for the reaction of the catalytic substrate in the living body. The application of enzymes is very extensive. The use of various biological enzymes can not only provide great help in the field of scientific research, but also greatly improve people's quality of life and even their health. However, enzymes are easily affected by various physical and chemical factors and lose their activity, and the sources of natural enzymes are limited and expensive, which requires high accuracy and fineness of experimental operations. Therefore, w...

Claims

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Application Information

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IPC IPC(8): B01J31/02B82Y30/00
CPCB01J31/0235B82Y30/00
Inventor 陈伟沈奕珉吴钢伟邓豪华彭花萍
Owner FUJIAN MEDICAL UNIV
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