Preparation method of SIS tissue repair material and application of preparation method

Abstract

The invention discloses a preparation method of an SIS tissue repair material and application of the preparation method. The preparation method of the SIS tissue repair material is characterized by comprising the following steps that 1, a small intestine is taken, a serosa layer, a mucosal layer and a muscular layer of jejunum are scraped away, and after the treated small intestine is cleaned with deionized water and cut off, small intestinal submucosa is obtained; 2, degreasing is conducted, the small intestinal submucosa obtained in the first step is put into a percolation tube, percolate with the weight 3-5 times of that of the small intestinal submucosa is added, soaking is conducted for at least 6 h, after soaking is completed, percolation is conducted, and percolate with the weight 20-30 times of that of the small intestinal submucosa is added, wherein the percolation speed is 1-2 ml / min, and the percolate is a mixed solution of ethyl alcohol and surfactant. According to the preparation method of the SIS tissue repair material and the application of the preparation method, the preparation method is free of toxicity and environmentally friendly, and the SIS repair capacity is high.

Description

technical field [0001] The invention relates to a preparation method and application of an SIS tissue repair material, belonging to the technical field of biomaterials. Background technique [0002] Small intestinal submucosa (smallintestinesubmucosa, SIS), one of the components of the small intestine, is a biodegradable natural extracellular matrix-like biologically derived material that contains a variety of growth factors, which play a role in tissue repair and reconstruction. important facilitation. [0003] At present, the preparation methods of SIS are mostly chemical methods, in which a large amount of organic solvents, surfactants and enzymes are mostly used. These substances have certain toxicity to growth factors, inactivate growth factors in SIS, destroy or reduce repair ability. In addition, organic solvents such as benzene and chloroform are often used in chemical methods, which not only makes the SIS preparation method highly toxic, endangers the health of op...

AI Technical Summary

Problems solved by technology

[0003] At present, the preparation methods of SIS are mostly chemical methods, in which a large amount of organic solvents, surfactants and enzymes are mostly used. These substances have certain toxicity to growth factors, inactivate growth factors in SIS, destroy or reduce Repair ability

In addition, organic solvents such as benzene and chloroform are often used in chemical methods, which not only makes the SIS preparation method highly toxic, endangers the health of operators, but also brings serious pollution to the environment.