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Preparation and application of magnetic resonance and near-infrared fluorescence targeted nanoprobes

A nano-probe and near-infrared technology, which is applied in the field of nuclear magnetic and fluorescence imaging nanomaterials, can solve the problems of probe tumor targeting and multi-modal multifunctional performance, so as to enhance MRI imaging ability and prolong the effective time , the effect of extending the window time

Inactive Publication Date: 2016-07-06
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Abstract
  • Description
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Problems solved by technology

[0006] At present, functional in vivo imaging nanoprobes are still insufficient in terms of probe tumor targeting and multi-modal multifunctional performance, and it is necessary to further construct new nanoprobes

Method used

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  • Preparation and application of magnetic resonance and near-infrared fluorescence targeted nanoprobes
  • Preparation and application of magnetic resonance and near-infrared fluorescence targeted nanoprobes
  • Preparation and application of magnetic resonance and near-infrared fluorescence targeted nanoprobes

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] MR&NIR-NPs preparation method:

[0033] (1) Synthesis of PEI-PEG-FA-DOTA-Gd carrier material

[0034] The EDC activated ester of PEG-COOH reacts covalently with PEI in a certain ratio (m / m=1:1) to prepare PEI-PEG, and then PEI-PEG is covalently grafted with TB-DOTA and FA through carboxyl esterification ( PEI-PEG:TB-DOTA:FA(m / m / m)=300:53.2:20.6), prepare PEI-PEG-FA-DOTA-TB, TFA / DMF deprotection, then chelate gadolinium acetate (feeding amount is Add 15.05 mg of gadolinium acetate per 100 mg of PEI-PEG-FA-DOTA-TB) to prepare PEI-PEG-FA-DOTA-Gd, and the infrared spectrum of the material is as follows figure 1 .

[0035] (2) Preparation of MR&NIR-NPs nanoparticles

[0036] Dissolve material PEI-PEG-FA-DOTA-Gd in deionized water or PBS (PH=7.4), concentration 2mg / ml, ultrasonic 100W ultrasonic 10s / pause 5s / 10 times, prepare PEI-PEG-FA-DOTA-Gd solution. The material ICG was dissolved in deionized water or PBS (PH=7.4) at a concentration of 1 mg / ml to prepare an ICG solu...

Embodiment 2

[0038] Characterization of MR&NIR-NPs:

[0039] (1) Morphology and size of MR&NIR-NPs

[0040] figure 2 It is a high-resolution transmission electron micrograph of MR&NIR-NPs, which shows that MR&NIR-NPs form a fibrous nanostructure with a narrow size distribution. image 3 It is a statistical analysis of the size distribution of electron microscope results. The results show that the average fiber diameter is 2.72nm, the average fiber length is 20.2nm, and the distribution is uniform.

[0041] (2) MR imaging performance evaluation of MR&NIR-NPs

[0042] Figure 4 is the MR imaging performance evaluation of MR&NIR-NPs. In the figure, the concentrations of MR & NIR-NPs are 1mg / ml, 0.5mg / ml, 0.25mg / ml, 0.125mg / ml, 0.0625mg / ml, 0mg / ml (the probes prepared in Example 1 were diluted with ultrapure water Obtain) MR imaging, imaging conditions: Tr=300ms, Te=19ms. The results show that the MR&NIR-NPs probe has good MR imaging ability.

[0043] (3) Evaluation of near-infrared fluo...

Embodiment 3

[0048] In vivo tumor targeted imaging of MR&NIR-NPs:

[0049] Figure 7 For MR&NIR-NPs targeted tumor MRI imaging results, Figure 8 It is the near-infrared fluorescence imaging results of MR&NIR-NPs targeting tumors. Imaging method: 7.5 mg / ml of MR&NIR-NPs solution and 7.5 mg / ml of control group (PPD-ICG) solution were prepared with normal saline as a solvent, and 100 μl per 10 g of nude mice body weight were injected into U87 glioma-bearing tumors through the tail vein in nude mice. At time points 0 (no injection), 24, 48, 72, and 96 h, MR imaging was performed under the conditions of Tr=300 ms, Te=19 ms, Av=2 times, slice thickness 2.5 mm, and nude mice were anesthetized during the process. . At the time points of 25min, 65min, 48h, 72h, and 96h, NIR imaging was performed under the conditions of 735nm excitation and 780nm-950nm acquisition, and nude mice were anesthetized during the process. The results show that MR&NIR-NPs have remarkable NMR and NIR targeted imaging ...

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Abstract

The invention discloses the preparation of Magnetic Resonance & Near-infrared Fluorescence Targeted Nanoprobes (MR & NIR-NPs) and its application in in vivo imaging and photothermal therapy. The skeleton material is selected, modified to reduce protein adhesion, grafted with nuclear magnetic imaging functional groups and tumor targeting groups, and then electrostatically self-assembled with near-infrared fluorescent dyes to form nanoprobes. group. The nuclear magnetic & near-infrared fluorescent targeting nanoprobe can target tumor tissue, has excellent MR and NIR multi-modal targeting imaging capabilities, and realizes photothermal therapy.

Description

technical field [0001] The invention relates to nuclear magnetic and fluorescence imaging nanomaterials, in particular to the preparation and application of a nuclear magnetic & near-infrared fluorescent nanoprobe. Background technique [0002] In nanotechnology, in vivo imaging technology based on near-infrared fluorescent materials or nuclear magnetic enhancement materials is one of the research frontiers and important development directions in the field of international biological detection, and it is also a scientific research hotspot in various countries. Among them, functional nanoprobe materials are a new growth point in the research of in vivo imaging technology. The focus of their application is currently focused on tissue target recognition, drug target delivery, and controlled and sustained release of kinetic materials. The reported nanoprobe in vivo imaging technology and existing problems include the following aspects: [0003] (1) Near-infrared fluorescent pro...

Claims

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Application Information

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IPC IPC(8): A61K41/00A61K49/00A61K49/12A61K49/10A61K49/14A61P35/00
Inventor 谭明乾吴昊马小军
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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