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Chorion decellularizing liquid and decellularizing method

A chorionic and decellularized technology, which is applied in medical science, tissue regeneration, prostheses, etc., can solve the problems of easy curling and falling off, difficult attachment, slow wound healing, etc., to achieve commercial production, not easy to curl, and widely popularized Applied effect

Active Publication Date: 2016-06-29
CHENGDU QINGKE BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, researchers have developed placental amniotic membrane, which has been widely used in medical treatment as a biomaterial after decellularization. However, there are disadvantages that the material is difficult to attach to tissues, cells are not easy to attach, and wound healing is slow.
For example, in the method disclosed in the patent CN1369555A, the prepared decellularized amniotic membrane is relatively thin, not easy to attach to the tissue, and prone to curling and falling off

Method used

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  • Chorion decellularizing liquid and decellularizing method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Step 1. Wash the placenta with 0.9% normal saline, and then peel off the chorion;

[0041] Step 2, put the chorion into the decellularized chorion preparation solution on a shaker, which includes 0.8% sodium dodecylsulfonate, 0.6% polyethylene glycol octylphenyl ether, 5% sodium chloride, 2% DNAse, 0.2% protease inhibitor, 0.8% trypsin, adjust the rotation speed to 200 rpm, shake at room temperature for 30 hours;

[0042] Step 3. Take out the chorion, wash it in pure water on a shaker, 200 rpm, shake at room temperature for 1 hour, repeat 8 times;

[0043] Step 4. Soak the cleaned chorion in 0.1 peracetic acid for 5 hours to sterilize it, and store it at 4°C for later use.

Embodiment 2

[0045] Step 1. Wash the placenta with 0.9% normal saline, and then peel off the chorion;

[0046] Step 2, put the chorion into the decellularized chorion preparation solution on the shaker, which includes 1% sodium dodecylsulfonate, 0.5% polyethylene glycol octylphenyl ether, 4% sodium chloride, 1.5% DNAse, 0.4% protease inhibitor, 0.6% trypsin, adjust the rotation speed to 160 rpm, shake at room temperature for 36 hours;

[0047] Step 3. Take out the chorion, wash it in pure water on a shaker, 160 rpm, shake at room temperature for 1 hour, repeat 7 times;

[0048] Step 4. Soak the cleaned chorion in 0.1 peracetic acid for 4 hours to sterilize it, and store it at 4°C for later use.

Embodiment 3

[0050] Step 1. Wash the placenta with 0.9% normal saline, and then peel off the chorion;

[0051] Step 2, put the chorion into the decellularized chorion preparation solution on the shaker, which includes 1% sodium dodecylsulfonate, 2% polyethylene glycol octylphenyl ether, 6% sodium chloride, 2.5% DNAse, 0.5% protease inhibitor, 1% trypsin, adjust the rotation speed to 180 rpm, shake at room temperature for 24 hours;

[0052] Step 3. Take out the chorion, wash it in pure water on a shaker, 180 rpm, shake at room temperature for 1 hour, repeat 8 times;

[0053] Step 4. Soak the cleaned chorion in 0.3 peracetic acid for 3 hours to sterilize it, and store it at 4°C for later use.

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PUM

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Abstract

The invention discloses chorion decellularizing liquid, which is prepared from the following ingredients: 0.5 to 1 part of dodecyl sodium sulfate, 0.5 to 2 parts of polyoxyethylene octyl phenyl ether, 3 to 6 parts of sodium chloride, 1 to 3 parts of DNAse (Deoxyribonuclease), 0.1 to 0.5 part of protease inhibitors and 0.4 to 1.2 parts of trypsinase. The chorion decellularizing liquid has the advantages that the defect that a chorion decellularizing preparation lacks in the prior art is overcome; by regulating the formula of the decellularizing liquid, a chorion product obtained through preparation reserves natural gaps and a fiber mesh structure; cell attachment is easy; curling cannot easily occur; the commercialized production is convenient; and the chorion decellularizing liquid can be widely popularized and applied.

Description

technical field [0001] The invention relates to the technical field of biological products, in particular to a chorion decellularization solution and a decellularization method. Background technique [0002] Decellularization technology mainly refers to the use of decellularization technology (physical, chemical, enzymatic and other methods) to remove various cellular components and genetic materials in tissues to obtain natural biological scaffold materials. Because it retains the three-dimensional structure and natural components of the extracellular matrix, it is conducive to cell adhesion, proliferation, differentiation and biological functions. Therefore, it has incomparable advantages in tissue repair and regeneration with other scaffold materials. At present, people have been able to obtain acellular matrix scaffold materials from a variety of tissues, such as small intestine, skin, blood vessels, cornea and more complex heart, lung, liver, kidney and so on. [0003]...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/36A61L27/58
CPCA61L27/3604A61L27/3683A61L27/58A61L2300/412A61L2430/40
Inventor 李俊黄海森张学超
Owner CHENGDU QINGKE BIOTECH
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