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Preparation method of high-calcium lactic acid bacteria fermented cucumber seed beverage

A technology of lactic acid bacteria fermentation and cucumber seeds, applied in the direction of lactobacillus, bacteria used in food preparation, and the function of food ingredients, etc., can solve the problems of few processed varieties, low calcium bioavailability, limited development level, etc.

Active Publication Date: 2016-06-22
SHENYANG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved by the present invention is to provide a preparation method of cucumber seed beverage fermented by high-calcium lactic acid bacteria, which solves the problems of limited development level of cucumber seed as a good food resource, few types of processed products, and low bioavailability of calcium contained in it.

Method used

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  • Preparation method of high-calcium lactic acid bacteria fermented cucumber seed beverage

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Select 20g of cucumber seeds and wash them for later use, add 1000mL of pure water (in order to eliminate the influence of water quality on the experimental results, pure water is used, but not limited to the use of pure water), 70g of sucrose is mixed and beaten with a beater, and then The colloid mill was homogenized for 3 times, and the obtained emulsion was divided into 200mL / bottles, covered, sterilized at 121°C for 15 minutes, and cooled for later use.

[0032] To prepare the fermented seed liquid of Lactobacillus germanus, first mix the MRS liquid medium and the cucumber seed emulsion in proportions of 10:0, 8:6, 5:5, 4:6, 2:8, and 0:10, and mix Lactobacillus germanus was transferred in turn, and the strains were acclimatized and cultured overnight at 34°C, so that the strains gradually adapted to grow in cucumber seed emulsion. The domesticated strains were placed in sterilized cucumber seed emulsion and cultured statically at 34°C, and the bacterial density was...

Embodiment 2

[0035] Choose 20g of cucumber seeds and wash for later use, add purified water (in order to eliminate the influence of water quality on the experimental results, the present invention adopts pure water, but not limited to the use of pure water) 1000mL, mix 70g of sucrose with a beater, and then use a colloid Grinding and homogenizing 3 times, the obtained emulsion was divided into 200mL / bottles, capped, sterilized at 121°C for 15 minutes, and cooled for later use.

[0036] To prepare the fermented seed liquid of Lactococcus plantarum, first mix the MRS liquid medium and cucumber seed emulsion in the ratio of 10:0, 8:6, 5:5, 4:6, 2:8, and 0:10, and mix the De Lactobacillus type was transferred in turn, and the strains were domesticated and cultured overnight at 30°C, so that the strains gradually adapted to grow in cucumber seed emulsion. The domesticated strains were placed in sterilized cucumber seed emulsion and cultured statically at 30°C, and the bacterial density was cont...

Embodiment 3

[0039] Choose 20g of cucumber seeds and wash for later use, add purified water (in order to eliminate the influence of water quality on the experimental results, the present invention adopts pure water, but not limited to the use of pure water) 1000mL, mix 70g of sucrose with a beater, and then use a colloid Grinding and homogenizing 3 times, the obtained emulsion was divided into 200mL / bottles, capped, sterilized at 121°C for 15 minutes, and cooled for later use.

[0040] To prepare the fermented seed liquid of Lactobacillus casei, first mix the MRS liquid medium and the cucumber seed emulsion at the ratio of 10:0, 8:6, 5:5, 4:6, 2:8, and 0:10, and mix the De Lactobacillus type was transferred in sequence, and the strains were domesticated and cultured overnight at 32°C, so that the strains gradually adapted to grow in cucumber seed emulsion. The acclimatized strains were placed in sterilized cucumber seed emulsion for static culture at 32°C, and the bacterial density was con...

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Abstract

The invention discloses a preparation method of a high-calcium lactic acid bacteria fermented cucumber seed beverage and belongs to the technical field of processing of agricultural products. A cucumber seed emulsion is prepared by taking cucumber seeds as raw materials, and a proper lactic acid bacteria strain is screened for fermenting to prepare an active lactic acid bacteria beverage; a product fermented by lactic acid bacteria contains protein with higher biological value, low fat and low sugar, wherein inorganic salts such as Ca, P, Fe and the like exist in a form of acid salts and are easily absorbed and utilized by the human body. The lactic acid bacteria fermented cucumber seed beverage has physiological activities of the lactic acid bacteria and metabolites thereof and has effects of cooling and refreshing and promoting the production of body fluid to relieve thirst endowed for common beverage; compared with the like plant lactobacillus fermented beverages, the high-calcium lactic acid bacteria fermented cucumber seed beverage has the most prominent characteristics that the content of soluble calcium in a product is greatly improved through a preparation process disclosed by the invention by using the characteristic that the cucumber seeds are rich in calcium, and a new path is provided for exerting biological effectiveness application to the maximum extent and improving the added value of the product.

Description

technical field [0001] The invention relates to the technical field of agricultural product processing, in particular to a method for preparing a cucumber seed beverage fermented by high-calcium lactic acid bacteria. Background technique [0002] Cucumber seeds are the mature seeds of Cucurbitaceae Cucumber genus Cucumber, containing a variety of free amino acids, fatty acids, vitamins and glycosides that are beneficial to the human body, as well as rich in calcium, phosphorus and other inorganic salts. In traditional Chinese medicine, it has the effects of strengthening tendons and bones, dispelling wind, and reducing inflammation. Its powder has a long history of application in bone setting, bone strengthening and calcium supplementation. However, the development of resources for both medicine and food in the food field is still very limited, and the product form is single. Some products that have appeared on the market in recent years are limited to cucumber seed powder a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L2/38A23L33/00
CPCA23L2/382A23V2002/00A23V2400/137A23V2400/125A23V2400/169A23V2250/1578A23V2200/322
Inventor 李苏红李拖平李欢欢马丹
Owner SHENYANG AGRI UNIV
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