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A method for improving the activity and tolerance of gastrointestinal tract of Streptococcus thermophilus and its application

A technology of Streptococcus thermophilus and tolerance, applied in the field of improving the activity of Streptococcus thermophilus and the tolerance of the digestive tract, can solve the problem that the number of probiotics in the product does not meet the standard, so as to reduce the heating time of the water bath, improve the activity, and achieve effective results fast effect

Active Publication Date: 2019-09-27
JIMEI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the industrial production of probiotic fermented products, there is often a problem that the number of probiotics in the product does not meet the standard

Method used

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  • A method for improving the activity and tolerance of gastrointestinal tract of Streptococcus thermophilus and its application
  • A method for improving the activity and tolerance of gastrointestinal tract of Streptococcus thermophilus and its application
  • A method for improving the activity and tolerance of gastrointestinal tract of Streptococcus thermophilus and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1: A method for preparing Gracilaria agar oligosaccharide grown by Streptococcus thermophilus

[0033] Step 1. Remove impurities from Gracilaria: wash and remove impurities from Gracilaria until there is no sediment and the water sample is clear, dry, add water 30 times the weight of Gracilaria to fully swell Gracilaria, and then heat in a water bath at 80°C 2h;

[0034] Step 2, autoclaving: Carry out autoclave sterilization to the Gracilaria after heating treatment, kill microorganism, autoclave temperature is 121 ℃, sterilization time is 20min;

[0035] Step 3, extruding glue: Squeeze Gracilaria while it is hot, wrap Gracilaria with filter cloth, and use a press to squeeze the filter cloth containing Gracilaria, collect the polysaccharide solution filtered out by extrusion; put the collected polysaccharide The solution is cooled, dried, crushed into granules and bagged for later use, and stored in a cool and dry place to obtain the Gracilaria crude polysaccha...

Embodiment 2

[0041] Example 2: The effect of agar oligosaccharides with different degrees of polymerization on improving the viability of Streptococcus thermophilus under low temperature storage

[0042] Agar oligosaccharides with different degrees of polymerization were prepared using the above method for preparing oligosaccharides, and added to the fermented oligosaccharides to reach OD 600 In the MRS medium of Streptococcus thermophilus with a value of 1.2, agar oligosaccharides (DP3, DP5, DP8) with different degrees of polymerization at the same concentration were added to the medium, and the culture conditions were anaerobic environment, 4°C, static Placed for culture, samples were taken every 3 days to observe the number of viable bacteria under a microscope, as shown in Table 1.

[0043] Table 1 Agar oligosaccharides with different degrees of polymerization improve the viability of Streptococcus thermophilus under low temperature storage

[0044]

[0045] The results showed that...

Embodiment 3

[0046] Example 3: Process flow for improving the viability of Streptococcus thermophilus under low temperature storage with different concentrations of Gracilaria agar oligosaccharides

[0047] Using the above method for preparing oligosaccharides, prepare different concentrations of agar oligosaccharides, and add them to the fermented oligosaccharides to reach OD 600 In the MRS medium of Streptococcus thermophilus with a value of 1.2, different concentrations of agar oligosaccharides (1g / L, 3g / L, 5g / L, 7g / L, 10g / L, 20g / L ) was added to the culture medium, and the culture conditions were anaerobic environment, 4°C, static culture, and samples were taken every 3 days to observe the number of viable bacteria under a microscope, as shown in Table 2.

[0048] Table 2 Different concentrations of Gracilaria agar oligosaccharides improve the activity of Streptococcus thermophilus under low temperature storage

[0049]

[0050]

[0051] The results showed that the protection ef...

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Abstract

The invention discloses a method for improving the activity of streptococcus thermophilus and the digestive tract tolerance. The method comprises the following steps that 1, agaro-oligosaccharide is prepared; 2, strain culture is conducted, wherein an MRS medium is prepared, the agaro-oligosaccharide serves as a carbon source, a streptococcus thermophilus liquid of which the OD<600> value is 0.8 is inoculated, and stationary culture is conducted for 2-3 days at 42 DEG C; 3, preservation of liquid bacteria is conducted, wherein in a streptococcus thermophilus liquid of which the OD<600> value is 1.2, agaro-oligosaccharide of which the concentration is 3 g / L and the degree of polymerization is 3 is added, and preservation is conducted at 4 DEG C; 4, preservation of a solid bacterial preparation is conducted, wherein in the streptococcus thermophilus liquid of which the OD<600> value is 1.2, the agaro-oligosaccharide of which the concentration is 3 g / L and the degree of polymerization is 3 is added, and the solid bacterial preparation is prepared through spray drying. According to the method for improving the activity of the streptococcus thermophilus and the digestive tract tolerance, the effect of improving the activity of the streptococcus thermophilus liquid and the solid bacterial preparation in the preservation process is achieved, the tolerance of the streptococcus thermophilus in gastrointestinal digestive tracts can also be improved, and the method can be used for lactobacillus fermentation enterprises and dairy product production enterprises to produce high-activity lactobacillus products and dairy products with high preservation resistance.

Description

technical field [0001] The invention relates to the technical field of food processing, in particular to a method for improving the activity and digestive tract tolerance of Streptococcus thermophilus and its application. Background technique [0002] Gracilaria agar oligosaccharide is a non-digestible oligosaccharide, which is prepared by hydrolyzing Gracilaria agar. It can inhibit the growth of pathogenic bacteria, spoilage bacteria and other harmful bacteria, improve human immunity, and improve human sub-health status. , has the effect of prolonging life. Therefore, Gracilaria agar oligosaccharide is a new type of health product and food additive or feed additive, which can significantly promote the growth of Streptococcus thermophilus, and its effect is explored when it is added to the medium of Streptococcus thermophilus as a prebiotic . [0003] At present, there is no report on the promoting effect of Gracilaria agar oligosaccharides on probiotics in the prior art. ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N1/04C12Q1/14C12R1/46
Inventor 肖安风吴昌正郑毅陈艳红杨秋明伍菱朱艳冰杨远帆李利君杜希萍肖琼
Owner JIMEI UNIV
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