A temperature-controlled capillary electrophoresis interface for chemiluminescent reactions

Abstract

The invention discloses a temperature-controlled capillary electrophoresis interface for a chemiluminescence reaction. The temperature-controlled capillary electrophoresis interface comprises a cannula module and an observation module, wherein the cannula module comprises a separating capillary tube, a high-voltage electrode, a luminescent reagent chamber, a reaction capillary tube and a water bath heating chamber; the separating capillary tube is used for injection and separation of to-be-detected articles and is inserted from one side of the cannula module, the reaction capillary tube is inserted from the other side of the cannula module, and the high-voltage electrode is adjacent to the inserting start end of the separating capillary tube; the separating capillary tube in the cannula module is inserted into the reaction capillary tube, and a gap is reserved between the inner wall of the separating capillary tube and the outer wall of the reaction capillary tube; a luminescent reagent inlet is formed in the luminescent reagent chamber; the start end of the reaction capillary tube is communicated with the luminescent reagent chamber to guide a luminescent reagent, the middle section of the reaction capillary tube penetrates through the water bath heating chamber, and a hot water inlet and a hot water outlet are formed in the water bath heating chamber; the part, behind an outlet in the tail end of the separating capillary tube, of the reaction capillary tube is a reaction area, and the reaction area is fixedly connected to the observation module. The temperature-controlled capillary electrophoresis interface is easy to assemble and manufacture, and temperature control of chemiluminescence detection is realized by means of a cannula structure.

Description

technical field [0001] The invention relates to a chemical reaction device, in particular to a temperature-controlled capillary electrophoresis interface for chemiluminescence reaction. Background technique [0002] Chemiluminescence (CL) detection does not require an external light source, has low background noise, high sensitivity (high sensitivity comparable to laser-induced fluorescence), wide linear range, fast analysis speed, and simple equipment. An efficient trace analysis technique that has been used in the analysis of biological samples. However, the complex composition of biological samples and the inherent poor selectivity of chemiluminescence limit the application of this method. Combining CL with high separation efficiency capillary electrophoresis (capillary electrophoresis, CE), it is directly used for the separation and determination of trace components in complex samples. Chance. CE-CL combined technology has been applied to the analysis of biological sa...

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Problems solved by technology

Dumke et al. immobilized luminol and horseradish peroxidase in the capillary, and modified the widely used commercial CE instrument for online detection of CL. Although the operability of CE-CL detection was improved, the sensitivity was lower than that of Most CE-CL detections are 2-3 orders of magnitude lower, and it is difficult to immobilize CL reagents in capillaries, and the reproducibility is not good enough

[0004] Although researchers have developed various CE-CL coupling interfaces, these interface devices are still in the stage of laboratory self-processing, have their own advantages and disadvantages, and cannot simultaneously solve the separation efficiency and sensitivity caused by the introduction of CL reagents. and stability reduction; the capillary is easily broken after hydrofluoric acid etching, and it is not easy to operate; the designed interface does not consider the influence of temperature on the CL reaction, and there is basically no temperature control device to control the temperature of the CL reaction, resulting in CL detection. The reproducibility is poor, and the detection sensitivity cannot be improved by increasing the CL reaction temperature

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