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Method for preparing smooth muscle cell carrier based on small intestine submucosa acellular matrix

A technology of small intestinal submucosa and smooth muscle cells, applied in the field of medical skin tissue engineering

Inactive Publication Date: 2016-06-15
苏州期佰生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is still a certain gap between polyglycolic acid materials and natural scaffold materials in terms of immunogenicity, antimicrobial properties, and ability to promote tissue regeneration.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] SIS preparation and histological examination:

[0026] Physical treatment: Small intestines are taken from domestic pigs with a body weight >200kg. The small intestine was frozen and refrigerated during transportation, and the cleaning process was completed within 4 hours. The serosa layer and muscular layer of the small intestine were removed with a knife handle wrapped in gauze, during which they were continuously rinsed with warm water at 40°C.

[0027] Chemical treatment: the small intestine treated by physical treatment was cut along the longitudinal axis, cut into 15cm-long sections, and prepared according to the method of Abraham et al. Each step of the chemical treatment process is carried out at room temperature, and the ratio of material to solution volume is kept at 1:100.

[0028]The specific method is: (1) Soak and stir in a solution containing 0.5mmol / L ethylenediaminetetraacetic acid (EDTA) and 0.4% trypsin at 37°C for 5.0~6.0h. (2) Continue to cross...

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Abstract

The invention discloses a method for preparing a smooth muscle cell carrier based on a small intestine submucosa acellular matrix. Vagina smooth muscle cells are successfully cultured in vitro, and the cultured vagina smooth muscle cells are seen to be in a long fusiform and gathered on a culture dish to form a typical peak and valley structure when invertedly placed under a microscope. The submucosa acellular matrix is white in appearance and translucent and has certain tenacity. Hematoxylin-eosin dying is conducted, and no cell component exists. After a vagina smooth muscle cell-intestine submucosa specimen section is dyed by hematoxylin-eosin, the number of visible cells under a light microscope is gradually increased, and the visible cells grow from the surface to the deep layer. After the vagina smooth muscle cell-intestine submucosa specimen section is dyed by an anti-rabbit smooth muscle alpha-actin monoclonal antibody in an immunohistochemical mode, positive cells of anti-rabbit alpha-Actin can be seen. The result preliminarily proves that the pig intestine submucosa acellular matrix can be taken as the smooth muscle cell carrier.

Description

technical field [0001] The invention relates to a method for preparing a smooth muscle cell carrier based on the acellular matrix of small intestinal submucosa, and belongs to the technical field of medical skin tissue engineering. Background technique [0002] Biologically derived materials have a structure similar to extracellular matrix and good biocompatibility, and are a very advantageous scaffold material for tissue engineering. Among many bio-derived materials, small intestinal submucosa (SIS) has the advantages of low immunogenicity, antibacterial properties and tissue growth promotion, so it is considered to be an ideal scaffold material for tissue engineering. [0003] SIS is rich in proteoglycan, collagen and fibronectin, which can promote the adhesion, proliferation and differentiation between cells and tissues. Proteoglycans often play a role in the early stages of cell adhesion, such as cell attachment and stretching stages; collagen passes through the α1 chai...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/38A61L27/36C12N5/071C12N5/077
CPCA61L27/3826A61L27/3629A61L27/3679A61L27/3683A61L27/3882A61L2430/22C12N5/0661C12N5/0682
Inventor 韩立军
Owner 苏州期佰生物技术有限公司
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