A double-stranded polynucleotide-ε-polylysine complex with immunoregulatory effect and its preparation and use method
A double-stranded polynucleotide and polylysine technology, applied in biochemical equipment and methods, medical preparations containing active ingredients, microorganisms, etc., can solve problems such as antibiotic residues and achieve strong resistance to biological enzymatic degradation , Improving immune regulation activity, and the effect of simple preparation process
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Embodiment 1
[0092] a, 0.1mol / L calcium chloride solution preparation:
[0093] Weigh 1.110 g of anhydrous calcium chloride and dissolve it in 100 ml of water for injection, and filter and sterilize through a filter membrane with a pore size of 0.2 μm to prepare a 0.1 mol / L calcium chloride solution.
[0094] b. Preparation of 0.01mol / L PBS solution (pH=7.6):
[0095] Weigh 0.124g of disodium hydrogen phosphate, 0.018g of sodium dihydrogen phosphate monohydrate, and 0.808g of sodium chloride respectively, dissolve them in 100ml of water for injection after mixing, and filter and sterilize through a filter membrane with a pore size of 0.2μm to make 0.01mol / L PBS solution (pH=7.6).
[0096] c. Preparation of 2mg / ml PolyIC intermediate solution:
[0097] Weigh 1.0088g of PolyI and 0.9912g of PolyC and add 400ml of PBS solution to dissolve, combine the two liquids, mix and shake well, add PBS solution to 1000ml, place in 45°C water bath for 60 minutes, and pass through a filter membrane with...
Embodiment 2
[0105] a, 0.1mol / L calcium chloride solution preparation:
[0106] Weigh 1.110 g of anhydrous calcium chloride and dissolve it in 100 ml of water for injection, and filter and sterilize through a filter membrane with a pore size of 0.2 μm to prepare a 0.1 mol / L calcium chloride solution.
[0107] b. Preparation of 0.01mol / L PBS solution (pH=7.6):
[0108] Weigh 0.124g of disodium hydrogen phosphate, 0.018g of sodium dihydrogen phosphate monohydrate, and 0.808g of sodium chloride respectively, dissolve them in 100ml of water for injection after mixing, and filter and sterilize through a filter membrane with a pore size of 0.2μm to make 0.01mol / L PBS solution (pH=7.6).
[0109] c, 2mg / ml preparation of bursal virus RNA solution:
[0110] The bursal virus RNA was dissolved in PBS buffer, and sterilized by filtration through a filter membrane with a pore size of 0.45 μm to prepare a 2 mg / ml solution for later use.
[0111] d. Preparation of 10mg / ml ε‐polylysine solution:
[01...
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