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Preparation method and application of evodia rutaecarpa serving as agricultural fungicide

A technology of Evodia and fungicides, applied in the field of Evodia fungicides and its preparation, can solve the problems of long research and development cycle and huge cost, and achieve the effects of low equipment requirements, long application history and simple processing.

Active Publication Date: 2016-06-15
INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Using traditional methods to develop a new pesticide requires screening tens of thousands of new compounds or more, which is costly and takes a long time to develop

Method used

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  • Preparation method and application of evodia rutaecarpa serving as agricultural fungicide
  • Preparation method and application of evodia rutaecarpa serving as agricultural fungicide
  • Preparation method and application of evodia rutaecarpa serving as agricultural fungicide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Evodia rutaecarpa fruit, stalk, and shell 5kg were extracted with 75% ethanol at 60°C for 3 times to obtain the total extract, which was adsorbed with A8 macroporous resin and eluted with water, 30%, 50%, and 75% ethanol respectively , collect 30%, 50% and 75% elution parts of the macroporous resin, concentrate the eluate to 1.5kg liquid extract, and obtain the total alkaloid extract of Evodia rutaecarpa. Put 0.5kg of surfactant (such as polysorbate-80) and 1.5kg of the total alkaloid extract of Evodia rutaecarpa into the homogenizer, heat to 30°C, and add 1kg of water into the homogenizer while stirring , stirred for 30 minutes, fully mixed, cooled to normal temperature, and formed water emulsion to obtain fungicide Evodia rutaecarpa.

Embodiment 2

[0032] 50kg of Evodia rutaecarpa leaves were extracted three times with 95% ethanol at 60°C to obtain the total extract, which was adsorbed with D101 macroporous resin, eluted with water, 30%, 50%, and 80% ethanol respectively, and the macroporous resin was collected for 30 %, 50% and 80% of the eluted parts, the eluate was concentrated to 1.5kg liquid extract to obtain the total alkaloid extract of Evodia rutaecarpa. Put 0.5kg of surfactant (such as polyethylene oxide non-ionic surfactant: OP-10) and 1.5kg of liquid extract of Evodia rutaecarpa total alkaloid extract into a homogenizer, heat to 50°C, and stir Add 1 kg of water into the homogenizer, stir for 30 minutes, fully mix, cool to normal temperature, form water emulsion, and obtain the bactericide of Evodia rutaecarpa.

Embodiment 3

[0034] Indoor toxicity measurement of the fungicide Evodia rutaecarpa prepared in Example 1:

[0035] Indoor virulence assay The tested plant pathogens include Sclerotinias clerotiorum, Fusarium graminearum, Botrytiscinerea, Colletotrichumgloeosporioides, Rhizoctoniasolani, corn Helminthosporium maydis, Botrytiscinerea, Fusarium moniliforme, Rhizoctoniacerealis, Alternariasolani, Verticilliumdahliae and rice blast 12 common plant pathogens including Magnaportheoryzae. All the tested pathogenic bacteria were isolated strains collected in the field.

[0036] The virulence of 12 kinds of plant pathogenic bacteria was determined by mycelial growth rate method. Each bacterial strain was activated and cultured on a PDA plate, and then a 5mm caliber punch was used to punch out a bacterial disk at the edge of the colony. The tested bactericide was made into a mother solution with sterile water, and then five drug-containing plates of PDA with concentration gradients were prepared b...

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Abstract

The invention relates to a plant source agricultural fungicide and a preparation method thereof. The fungicide is formed by extracting and processing fruit, carpopodium, shells and leaves of evodia rutaecarpa. The fungicide comprises the following main active ingredients in percentage by weight: 6 to 10 percent of hydroxyevodiamine, 5 to 10 percent of evodiamine, 5 to 10 percent of rutaecarpin, 6 to 10 percent of dehydroevodiamine, 1 to 5 percent of bievodiamine, 1 to 5 percent of hepta evodiamine, 6 to 40 percent of other alkaloid components. The main active ingredients are processed and manufactured into an emulsion in water, and can be used for controlling and preventing a plurality of plant diseases after being diluted. The plant source agricultural fungicide and the preparation method thereof have the advantages that raw materials are easily obtained; the process is simple; the cost is low; the fungicide has very good control and prevention effects on a plurality of plant diseases, such as sclerotinia rot of colza, wheat scab and grape anthracnose, is safe to plants, does not pollute the environment, and is a biological pesticide preparation which meets the production requirements on pollution-free agricultural products in China.

Description

1. Technical field [0001] The invention relates to a fungicide of evodia rutaecarpa and a preparation method thereof. 2. Background technology [0002] Relevant experts believe that China should vigorously develop botanical pesticides. At present, more than 80% of the pesticides used in China are organic synthetic pesticides. The large-scale use of highly toxic and high-residue organic synthetic pesticides has led to serious environmental pollution, ecological balance damage, and pathogen resistance. As people's awareness of advocating nature, protecting the environment, and paying attention to food safety is increasing, the pollution-free biological pesticide industry and the field of biological control research have obtained rare development opportunities. Therefore, the development of botanical pesticides has become a new way for the development of Chinese pesticides and Chinese herbal medicines. To develop a new pesticide using traditional methods, it is necessary to s...

Claims

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Application Information

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IPC IPC(8): A01N65/36A01N43/90A01P3/00
CPCA01N43/90A01N65/36
Inventor 徐曙王奇志冯煦王碧刘飞陈雨单宇印敏管福琴王鸣赵友谊张建华
Owner INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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