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Diluent enabling stability for acridinium ester antigen-antibody conjugate and preparation method of diluent

An antigen-antibody and conjugate technology, applied in the field of chemical analysis, can solve the problems of decreased stability of reagents, shortened shelf life of reagents, unstable acridine esters, etc., and achieves the effect of prolonging shelf life, maintaining use validity, and not easy to hydrolyze

Active Publication Date: 2016-06-01
GUANGZHOU KEFEN BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] From the current technical level, acridinium esters are unstable and easily hydrolyzed in general buffer solutions. When the conjugates are diluted to the working concentration, the stability of the reagents will decrease due to the decrease in the concentration of the conjugates in the diluent. resulting in shortened shelf life of reagents

Method used

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  • Diluent enabling stability for acridinium ester antigen-antibody conjugate and preparation method of diluent
  • Diluent enabling stability for acridinium ester antigen-antibody conjugate and preparation method of diluent
  • Diluent enabling stability for acridinium ester antigen-antibody conjugate and preparation method of diluent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] A diluent capable of stabilizing the acridinium ester antigen-antibody conjugate, which is prepared from the following components in mass percentage:

[0040]

[0041] The preparation method of the diluent is as follows:

[0042] 1) Add sucrose, trehalose, glycerin, glycine, BSA, DTPA, Tween-20, sodium azide, PEG6000 and sodium chloride into 0.05mol / L citrate buffer solution according to the formula, stir until completely dissolved, and get mixed solution;

[0043] 2) Stir the mixed solution obtained in step 1), and add NaOH to adjust the pH value to 6.4±0.1 to obtain a diluted solution.

[0044] Dilute the following conjugates with the diluent prepared in Example 1, the conjugates are respectively acridinium ester-FSH antibody and acridinium ester-E2 antigen, and dilute to the working concentration to obtain acridinium ester-FSH antibody dilution (1) and acridinium ester-E2 antigen diluent (1). 13 vials of each of the two diluted conjugates were dispensed, about ...

Embodiment 2

[0046] A diluent capable of stabilizing the acridinium ester antigen-antibody conjugate, which is prepared from the following components in mass percentage:

[0047]

[0048]

[0049] The preparation method of the diluent is as follows:

[0050] 1) Add sucrose, trehalose, glycerin, glycine, BSA, DTPA, Tween-20, sodium azide, PEG6000 and sodium chloride into 0.2mol / L citrate buffer solution according to the formula, stir until completely dissolved, and get mixed solution;

[0051] 2) Stir the mixed solution obtained in step 1), and add NaOH to adjust the pH value to 6.4±0.1 to obtain a diluted solution.

[0052]Dilute the following conjugates with the diluent prepared in Example 2, the conjugates are acridinium ester-FSH antibody and acridinium ester-E2 antigen respectively, and dilute to the working concentration to obtain acridinium ester-FSH antibody dilution (2) and acridinium ester-E2 antigen diluent (2). 13 vials of each of the two diluted conjugates were dispens...

Embodiment 3

[0054] A diluent capable of stabilizing the acridinium ester antigen-antibody conjugate, which is prepared from the following components in mass percentage:

[0055]

[0056]

[0057] The preparation method of the diluent is as follows:

[0058] 1) Add sucrose, trehalose, glycerin, glycine, BSA, DTPA, Tween-20, sodium azide, PEG6000 and sodium chloride into 0.1mol / L citrate buffer solution according to the formula, stir until completely dissolved, and get mixed solution;

[0059] 2) Stir the mixed solution obtained in step 1), and add NaOH to adjust the pH value to 6.4±0.1 to obtain a diluted solution.

[0060] Dilute the following conjugates with the diluent prepared in Example 3, the conjugates are respectively acridinium ester-FSH antibody and acridinium ester-E2 antigen, and dilute to the working concentration to obtain the acridinium ester-FSH antibody dilution (3) and acridinium ester-E2 antigen diluent (3). 13 vials of each of the two diluted conjugates were di...

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Abstract

The invention discloses a diluent enabling stability for acridinium ester antigen-antibody conjugate. The diluent is prepared by adding a carbohydrate, polyalcohol, amino acids, a protein stabilizer, a chelating agent, a surfactant, a preservative and an additive into a buffer solution; the diluent prepared by the invention overcomes the defect that acridinium ester is unstable and easily hydrolyzes in the buffer solution, can protect the acridinium ester antigen-antibody conjugate to improve its stability and prolong its service life, and also can be fully used in in-vivo diagnostic kits.

Description

technical field [0001] The invention belongs to the technical field of chemical analysis, and in particular relates to a diluent capable of stabilizing an acridinium ester antigen-antibody conjugate and a preparation method thereof. Background technique [0002] Chemiluminescent immunoassay is an immunoassay that uses chemiluminescent agents to directly label antigens or antibodies. Acridinium esters are ideal luminescent substrates. Under alkaline conditions, acridinium ester molecules are attacked by hydrogen peroxide to generate dioxygen Ethane, dioxyethane is unstable and decomposes into CO 2 and N-methylacridone in an electronically excited state, which emits light at a wavelength of 430 nm when it returns to the ground state. [0003] The acridinium ester marker has a special group that produces luminescence in its chemical structure, and directly participates in the luminescence reaction in the process of luminescence immunoassay. Usually, this kind of substance has...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/53
CPCG01N33/53
Inventor 王敏仪
Owner GUANGZHOU KEFEN BIOTECH CO LTD
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