Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A kind of peony seed hypoglycemic peptide and its purification method and application

A purification method and technology of peony seeds, which are applied in the field of peony seed hypoglycemic peptides and their purification, can solve the problems of low activity of peptides, achieve the effects of large adsorption capacity, low cost, and increased product yield

Active Publication Date: 2018-11-16
山东聚胜生物科技有限公司
View PDF4 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the activity of these peptides is not high. For example, the two tripeptides isolated from silk-derived hypoglycemic peptides have half-inhibitory doses of 2.7mg / mL and 1.5mg / mL respectively.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A kind of peony seed hypoglycemic peptide and its purification method and application
  • A kind of peony seed hypoglycemic peptide and its purification method and application
  • A kind of peony seed hypoglycemic peptide and its purification method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Proportion the raw materials and distilled water according to the material-to-liquid ratio of 1:10, stir for 15 minutes to fully mix, adjust the pH of the solution to 9.5 with 1mol / L NaOH, ultrasonicate in a water bath at 50°C for 2 hours, and centrifuge at 3800r / min for 15 to 20 minutes, and the obtained precipitate is again Extract twice, combine the obtained upper liquid to adjust the pH to 4.5 with 1mol / L HCl, centrifuge at 3800r / min for 15-20min, take the precipitate and freeze-dry to obtain the peony seed protein.

Embodiment 2～13

[0036] The enzymatic hydrolysis reaction conditions are as follows: prepare a peony seed protein solution with a concentration of 1-3%, add enzyme amount of 5000-12500U / g, pH 7.5-8.5, temperature 35-40°C, time 15-25min, ultrasonic frequency 28kHz, power 100W / L, boiling water bath for 10 minutes after the end of the enzymolysis reaction, the peony seed polypeptide enzymatic hydrolysis solution can be obtained.

[0037] In vitro hypoglycemic assay method:

[0038] (1) Preparation of mouse-derived α-glucosidase

[0039] The mice were sacrificed by dislocation, the impurities were washed away with 0°C normal saline, and ground with liquid nitrogen. Mix PBS with grinding powder, mix according to the ratio of 10:1 by volume and mass, extract by vortex for 60s, centrifuge at 8000r / min at 4°C for 15min, and take the supernatant.

[0040] (2) α-glucosidase inhibition rate detection method

[0041]

[0042] React in a water bath at 37°C for 15 minutes, and detect the absorbance a...

Embodiment 14

[0055] Centrifuge the polypeptide enzymatic solution obtained in Example 13 at 8000r / min for 15min, take the clear liquid in the middle layer, and filter it with a 0.46μm filter membrane. , and then use a 5000Da filter membrane to carry out secondary ultrafiltration on the primary filtration solution, and finally select the filtrate below 5000Da with the best inhibition rate and save it for later use;

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses peony seed blood sugar decreasing peptide. A preparation method of the peony seed blood sugar decreasing peptide includes the steps that 1, peony seed spent meal and water are mixed to be uniform, protein is extracted through ultrasonic stirring, centrifugation is conducted, obtained precipitate is repeatedly extracted twice, supernatant liquid is combined, 1 mol / L HCl is used for adjusting the pH value to be 4 to 4.5, centrifugation is conducted, precipitate is taken for freeze drying, and peony seed protein is obtained; 2, a 1-3% peony seed protein solution is prepared, trypsin is used for ultrasonic-assisted enzymolysis, a reaction is completed, a boiling water bath is conducted for 10-15 min for enzyme deactivation, and then the peony seed blood sugar decreasing peptide can be obtained. The invention further provides a purification method and application of the penny seed blood sugar decreasing peptide. The peony seed protein is degraded through an ultrasonic-assisted biological enzyme method and cut into multiple kinds of polypeptide with different molecular weights, separation and purification are conducted through ultra-filtration, ion exchange, sephadex and high performance liquid chromatography, an enzyme digestion reaction can be efficiently conducted, a large amount of peony seed blood sugar decreasing peptide is obtained, the activity of the polypeptide can be kept not lost in the purification process, and production and utilization are convenient.

Description

technical field [0001] The invention belongs to the field of food biotechnology, and in particular relates to a peony seed hypoglycemic peptide and its purification method and application. Background technique [0002] Diabetes is a disease that seriously threatens human health. With the rapid development of the economic level, people's diet is getting better and better, and the amount of exercise is decreasing day by day, leading to an increase in the incidence of diabetes year by year. Traditional hypoglycemic drugs include glucosidase inhibitors, insulin sensitizers, insulin secretagogues, Chinese patent medicines, insulin, biguanides, sulfonylureas, etc. Due to the side effects of drug treatment, long-term use is difficult for patients to accept. In recent years, the method of controlling blood sugar through dietary therapy has become more and more popular, among which the development of new α-glucosidase inhibitors is the direction of efforts of many scholars. [0003...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12P21/06C07K1/36C07K1/34C07K1/20C07K1/18C07K1/16A61K38/16A61P3/10A23L33/18A23L33/185
CPCA61K38/00C07K14/415C12P21/06
Inventor 颜辉蔡豪贾俊强吴琼英熊孟江明珠
Owner 山东聚胜生物科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com