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Fly maggot heat-resistant protein extract and preparation method and application thereof

A technology of protein extracts and fly maggots, which is applied in the field of medicine, can solve the problems of reducing medicinal activity, affecting drug absorption, and unfixed types of fly maggots, so as to reduce the loss of effective substances, simplify the preparation process, and improve the medicinal effect Effect

Inactive Publication Date: 2016-06-01
ZHONGSHAN UNICARE NATURAL MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most of the grain worms traditionally used as medicine are raised as toad meat, which requires high feeding conditions, which greatly increases the cost of raw materials for the grain worms
Simple drying and grinding into medicine can not give full play to the medicinal value of fly maggots
And fresh wild fly maggots are fed to poultry and livestock, and there are also problems such as unqualified hygienic conditions, unfixed fly maggot types, unstable anti-diarrhea effect, etc.
And some studies have shown that dried fly maggots contain about 15% to 25% oil, and the oil itself has the effect of laxative and smooth intestines. Therefore, the traditional usage of fly maggots directly ground into medicine cannot effectively exert its antidiarrheal effect
There are also studies that show that the fly maggot skin contains a large amount of chitin and chitin to protect itself (Qin Ronggui, Wu Jianwei, Guoguo, etc. Research on the preparation technology of fly maggot chitin [J]. Lishizhen Medicine and Materia Medica Research, 2010 (05) : 1189-1191.), the simple way of drying and grinding into powder for medicine cannot fully release the effective substances in the body, thereby affecting drug absorption and reducing medicinal activity

Method used

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  • Fly maggot heat-resistant protein extract and preparation method and application thereof
  • Fly maggot heat-resistant protein extract and preparation method and application thereof
  • Fly maggot heat-resistant protein extract and preparation method and application thereof

Examples

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Effect test

Embodiment 1

[0036] The preparation method of the fly maggot heat-resistant protein extract of the present embodiment comprises the following steps:

[0037] (1) Rinse and disinfect: put fresh fly maggots into a cloth bag, rinse repeatedly in running water, so that the contents of the worm body are completely removed, and the fly maggots are cleaned and then disinfected;

[0038] (2) Extraction of crude protein: add 5 times the weight of fly maggots in advance pre-cooled to 4°C extract solution I, fully homogenate at 4°C, and freeze and centrifuge the obtained homogenate at 15,000 rpm for 50 minutes at 4°C, discard the upper layer of oil and Insoluble precipitation, the intermediate clear solution is obtained for subsequent use; the extract I is: a buffer solution of sodium dihydrogen phosphate-disodium hydrogen phosphate (PH=8), which contains 1% glycerol by volume fraction, and 1% by mass fraction Sodium chloride, 10mmol / L dipotassium ethylenediaminetetraacetic acid (EDTA-K 2 ), a volum...

Embodiment 2

[0042] The preparation method of the fly maggot heat-resistant protein extract of the present embodiment comprises the following steps:

[0043] (1) Rinse and disinfect: put fresh fly maggots into a cloth bag, rinse repeatedly in running water, so that the contents of the worm body are completely removed, and the fly maggots are cleaned and then disinfected;

[0044] (2) Extraction of crude protein: add 10 times the weight of fly maggots to the extraction solution I pre-cooled to 4°C in advance, fully homogenate at 4°C, and freeze and centrifuge the obtained homogenate at 20,000 rpm for 10 minutes at 4°C, discard the upper layer of oil and Insoluble precipitation, the intermediate clear solution is obtained for subsequent use; the extract I is: sodium dihydrogen phosphate-disodium hydrogen phosphate buffer solution (PH=6), which contains 50% glycerin by volume fraction and 0.1% chlorine by mass fraction NaCl, 5mmol / LEDTA-K 2 , a volume fraction of 0.1% β-mercaptoethanol;

[...

Embodiment 3

[0048] The preparation method of the fly maggot heat-resistant protein extract of the present embodiment comprises the following steps:

[0049] (1) Rinse and disinfect: put fresh fly maggots into a cloth bag, rinse repeatedly in running water, so that the contents of the worm body are completely removed, and the fly maggots are cleaned and then disinfected;

[0050] (2) Extraction of crude protein: add 15 times the weight of fly maggots in advance and pre-cooled to 4°C extract Ⅰ to fully homogenate at 4°C, and the resulting homogenate is refrigerated and centrifuged at 10,000 rpm for 20 minutes at 4°C, and the upper layer of oil and insoluble precipitates are discarded , to get the intermediate clear liquid for subsequent use; the extract I is: sodium dihydrogen phosphate-disodium hydrogen phosphate buffer solution (PH=6), which contains 10% glycerin by volume fraction and 0.5% sodium chloride by mass fraction , 0.1mmol / LEDTA-K 2 , a volume fraction of 5% beta-mercaptoethano...

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Abstract

The invention relates to a fly maggot heat-resistant protein extract and a preparation method thereof. The fly maggot heat-resistant protein extract prepared by the steps of rinsing and sterilizing of fresh fly maggots, extraction of crude protein, removal of heat-labile protein, desalination, freeze-drying and the like has remarkable anti-diarrhea effect. According to the preparation method, loss of effective substance of fly maggots during conventional processes such as sun-drying is reduced greatly since fresh fly maggots are used as medicine directly; extracting solution contains essential substances such as buffer salt and antioxidant which can effectively prevent inactivation of the effective substances; fly maggots are homogenized completely by the extracting solution in rational proportion so that cells of fly maggots are ruptured, the effective substances in bodies of the fly maggots are released completely, and dissolution of the effective substances of the fly maggots is improved; and anti-diarrhea effect of the fly maggot heat-resistant protein extract is improved further by procedures of centrifugal degreasing, impurity removal, removal of heat-labile components by water bath and the like after homogenization.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to a fly maggot heat-resistant protein extract and its preparation method and application. Background technique [0002] Diarrhea is a gastrointestinal disease caused by multiple pathogens and multiple factors. Its main symptoms are that the frequency of defecation is significantly higher than the frequency of usual habits, the fecal matter is thin, the water content increases, the daily defecation volume exceeds 200g, or contains undigested food or pus and blood. , mucus, often accompanied by a sense of urgency to defecate, anal discomfort, incontinence, etc. Diarrhea has a high incidence rate and is extremely harmful to children's health. It is a worldwide public health problem. The Ministry of Health of my country also lists diarrhea as one of the key diseases. According to the data released by the World Health Organization, there are as many as 17 million cases of diarrhea wor...

Claims

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Application Information

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IPC IPC(8): C07K1/14C07K1/34A61K38/17A61K9/48A61K9/16A61P1/12
CPCC07K1/145A61K9/1652A61K9/4858A61K35/63A61K38/00C07K1/34
Inventor 杨文哲牛艺郑冬丛佩青牛惠然林丽杨得坡
Owner ZHONGSHAN UNICARE NATURAL MEDICINE
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