Method for preparing salt-resistant alkali-resistant special algal biological water-soluble fertilizer by enzymatic hydrolysis fermentation
The invention relates to a fermentation method and a salt-alkali resistance technology, which is applied in the field of the preparation of salt-alkali-resistant seaweed biological water-soluble fertilizers by an enzymatic hydrolysis and fermentation method. Effect
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[0020] The present invention provides a specific embodiment of a method for preparing seaweed biological water-soluble fertilizer special for saline-alkali resistance by enzymatic hydrolysis and fermentation, which specifically includes the following steps:
[0021] (1) Seaweed pretreatment: wash the seaweed with clean water, remove impurities, drain; dry at 80-90°C, finally grind and sieve;
[0022] (2) Ultrasonic-assisted enzymolysis: add water 8 to 10 times the mass of seaweed, and adjust the pH to 6.8 to 7.2; Ultrasonic-assisted enzymatic hydrolysis at 60°C for 2-4 hours;
[0023] (3) Fermentation broth preparation: add 3.0% to 5.0% glucose, 2.0% to 3.0% yeast extract, 0.3% to 0.5% potassium dihydrogen phosphate, and 0.02% MgSO to the enzymolysis solution 4 .7H 2 O, 0.01%CaCl 2 , adjust the pH to 7.0-7.2;
[0024] (4) Aerobic fermentation: use a liquid fermentation tank with a liquid volume of 60% to 70%, inoculate Bacillus pyrolyticum and Aspergillus niger, and inocul...
Embodiment 1
[0030] A method for preparing seaweed biological water-soluble fertilizer special for saline-alkali resistance by enzymolysis and fermentation, the specific preparation steps are as follows:
[0031] (1) Seaweed pretreatment: wash the seaweed with clean water, remove impurities, and drain; dry at 85°C, finally grind into powder, and pass through a 40-mesh sieve;
[0032] (2) Ultrasonic-assisted enzymolysis: add water 10 times the mass of seaweed, adjust the pH to 6.8-7.2; add 3% glucoamylase and 2% non-starch polysaccharase according to the mass ratio of seaweed, and ultrasonic-assisted enzymolysis at 40-60°C for 2 hours, Ultrasonic power 400w, frequency 25kHz;
[0033] (3) Fermentation broth preparation: Add 5.0% glucose, 3.0% yeast extract, 0.5% potassium dihydrogen phosphate, and 0.02% MgSO to the enzymolysis solution 4 .7H 2 O, 0.01%CaCl 2 , adjust the pH to 7.0-7.2;
[0034] (4) Aerobic fermentation: select a liquid fermentation tank with a liquid volume of 60% to 70%...
Embodiment 2
[0039] A method for preparing seaweed biological water-soluble fertilizer special for saline-alkali resistance by enzymolysis and fermentation, the specific preparation steps are as follows:
[0040] (1) Seaweed pretreatment: wash the seaweed with clean water, remove impurities, and drain; dry at 85°C, finally grind into powder, and pass through a 40-mesh sieve;
[0041] (2) Ultrasonic-assisted enzymolysis: add water 8 times the mass of seaweed, adjust the pH to 6.8-7.2; add 2% glucoamylase and 1% non-starch polysaccharase according to the mass ratio of seaweed, and ultrasonic-assisted enzymolysis at 40-60°C for 4 hours, Ultrasonic power 400w, frequency 25kHz;
[0042] (3) Fermentation broth preparation: Add 3.0% glucose, 2.0% yeast extract, 0.3% potassium dihydrogen phosphate, and 0.02% MgSO to the enzymolysis solution 4 .7H 2 O, 0.01%CaCl 2 , adjust the pH to 7.0-7.2;
[0043] (4) Aerobic fermentation: use a liquid fermentation tank with a liquid volume of 60% to 70%, in...
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