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Method for producing microbial oil through oil-producing microorganisms adopting jerusalem artichoke as raw materials

A technology for oil-producing microorganisms and microbial oils, which is applied in the direction of single-cell algae, bacteria, fungi, etc., can solve problems such as the inability to use nitrogen limitation regulation, difficulty in removing nitrogen elements from Jerusalem artichoke raw materials, and accumulation of oil, so as to improve oil production intensity and Substrate conversion rate, improving microbial oil fermentation technology economy, and improving the effect of utilization

Inactive Publication Date: 2016-05-18
DALIAN POLYTECHNIC UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The object of the present invention is to develop a method for cultivating oil-producing microorganisms using Jerusalem artichoke as a raw material, which can increase the accumulation rate of bacterial fats and realize efficient synthesis of oils; solve the difficulty of removing nitrogen elements from Jerusalem artichoke raw materials, and cannot use nitrogen limitation to regulate accumulation To solve the oil problem, further improve the oil production intensity and substrate conversion rate, realize the effective utilization of culture raw materials, reduce production costs, improve the economics of microbial oil fermentation technology, and realize large-scale industrial production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] (1) Preparation of seed medium: Take 100 mesh inulin, 50g inulin and mix water with a mass ratio of 1:10, add water to make the volume 500ml, use 3mol / L hydrochloric acid to adjust the pH to 3.6; hydrolyze under saturated steam for 30min, adjust the pH To neutrality, obtain the Jerusalem artichoke hydrolyzate, prepare another part of Jerusalem artichoke hydrolyzate according to the same method, in order to use in step (3); Get wherein a part of Jerusalem artichoke hydrolyzate, add yeast powder to 10g / L, potassium dihydrogen phosphate to 1.0g / L, MgSO 4 ·7H 2 0 to 0.5g / L, sterilized to obtain the seed culture medium of microorganisms;

[0048] (2) Bacteria proliferation culture: with 10% (V / V) (OD 600nm =7.8) inoculum size Trichosporonfermentans CICC1368 (purchased from China General Microorganism Culture Collection Management Center) was connected to the seed medium prepared in step (1), 28°C, 100rpm, cultivated for 24h, and centrifuged to obtain Oleaginous microbial ...

Embodiment 2

[0058] (1) Preparation of seed medium: Take 50 mesh inulin, 70g inulin, and mix with water at a mass ratio of 1:4.3, add water to make the volume 300ml, use 3mol / L hydrochloric acid to adjust the pH to 2.0; hydrolyze under saturated steam for 20min, adjust the pH To neutrality, the Jerusalem artichoke hydrolyzate is obtained, and another part of the Jerusalem artichoke hydrolyzate is prepared in the same way for use in step (3); take one part of the Jerusalem artichoke hydrolyzate, add peptone 5g / L, and potassium dihydrogen phosphate to 1.0g / L,MgSO 4 ·7H 2 0 to 0.5g / L, sterilized to obtain the seed culture medium of microorganisms;

[0059] (2) Bacteria proliferation culture: with 10% (V / V) (OD 600nm =6.5) Inoculum size Cryptococcus curvatusATCC20509 (purchased from the American Type Culture Collection Center) was connected to the seed medium prepared in step (1), 24 ° C, 150 rpm, cultured for 24 hours, and centrifuged to obtain oil-producing microbial cells;

[0060] (3)...

Embodiment 3

[0069] (1) Preparation of seed medium: Take 200 mesh inulin, 100g inulin and mix with water at a mass ratio of 1:5, add water to make the volume 500ml, use 3mol / L hydrochloric acid to adjust the pH to 2.5; hydrolyze under saturated steam for 10min, adjust the pH To neutrality, obtain the Jerusalem artichoke hydrolyzate, prepare another part of Jerusalem artichoke hydrolyzate according to the same method, in order to use in step (3); Get wherein a part of Jerusalem artichoke hydrolyzate, add NH 4 NO 3 5g / L, potassium dihydrogen phosphate to 1.0g / L, MgSO 4 ·7H 2 0 to 0.5g / L, sterilized to obtain the seed culture medium of microorganisms;

[0070] (2) Bacteria proliferation culture: with 10% (V / V) (OD 600nm=5.0) inoculum size Rhodococcus opacus PD630 (purchased from the German Microorganism Culture Collection Center) was connected to the seed medium prepared in step (1), 30°C, 200rpm, cultivated for 48h, and centrifuged to obtain oil-producing microorganisms bacteria;

[007...

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Abstract

The invention discloses a method for cultivating oil-producing microorganisms adopting jerusalem artichoke as raw materials. The method comprises the following steps of preparing a seed culture medium, proliferating and cultivating thallus, preparing an oil-producing culture medium, culturing thallus oil in an accumulated mode, extracting oil-producing microorganism oil and the like. Inulin is adopted as the raw materials for preparing the culture medium, the cost is low, and the method is applicable to large-scale industrial application; according to characteristics of a jerusalem artichoke hydrolysate and different processing modes, the content of nutrient elements is adjusted, the culture technology for phosphorus-limiting / sulfur-control combined two-stage culture of oil-producing microorganisms is developed, the thallus proliferation speed and the oil accumulation rate of the oil-producing microorganisms are remarkably improved, and the oil yield is remarkably improved. Due to the application, the biodiesel oil raw material sources are enlarged, the production period can be remarkably shortened, the production cost is lowered, and the production efficiency is improved.

Description

technical field [0001] The invention relates to the technical field of oil-containing microorganism cultivation and microbial oil production, and specifically relates to a method for cultivating oil-containing microorganisms using inulin as a raw material, phosphorus / sulfur limitation and regulation combined with a two-stage method, and using the microorganisms to produce microbial oil. Background technique [0002] Microbial oil, also known as single cell oil (SCO), is composed of oil-producing microorganisms such as yeast, mold, bacteria, and algae, which use carbohydrates, hydrocarbons, and ordinary oils as carbon sources, nitrogen sources, and supplemented by inorganic salts. Grease produced under certain conditions. Compared with animal and vegetable oils, microbial oils have a short production cycle, are not restricted by seasons and climates, have a wide source of raw materials, basically do not occupy additional arable land resources, and are easy to achieve large-sc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/64C12N1/20C12N1/14C12N1/12
CPCC12P7/6463C12N1/12C12N1/14C12N1/20
Inventor 林心萍谢同舟高宁孙黎明纪超凡刘洒洒朱容仟
Owner DALIAN POLYTECHNIC UNIVERSITY
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