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Quick deep-layer liquid state fermentation method for producing sparassis crispa bacterial powder

A technology of submerged liquid fermentation and Hydrangea, which is applied in the field of microorganisms, can solve the problems of heavy workload in the sewage treatment system, decline in product quality and yield, large consumption of manpower and material resources, etc., to improve equipment utilization, filtrate clarification, and installation The effect of easy maintenance

Inactive Publication Date: 2016-05-18
JIANGSU SHENHUA PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The traditional hydrangea liquid fermentation process is mostly an intermittent batch fermentation process, but the batch fermentation process consumes a lot of manpower and material resources. Each batch of fermentation needs to be loaded, sterilized, inoculated, discharged, and cleaned. The process is cumbersome, the non-fermentation cycle is long, and the production efficiency is low; while the subsequent separation process is traditionally separated by a centrifuge, this method is subject to the influence of the separation factor of the centrifuge, and the mycelium is damaged by shear force during the separation process. The loss of some active ingredients into the filtrate leads to a decrease in product quality and yield. The separated filtrate is turbid and contains more broken mycelium, resulting in a high COD of the discharged filtrate. If the filtrate meets the sewage discharge standard, it needs to go through complicated procedures. Sewage treatment, the workload of the sewage treatment system is too large, which brings a serious economic burden to the enterprise

Method used

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  • Quick deep-layer liquid state fermentation method for producing sparassis crispa bacterial powder
  • Quick deep-layer liquid state fermentation method for producing sparassis crispa bacterial powder
  • Quick deep-layer liquid state fermentation method for producing sparassis crispa bacterial powder

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Embodiment 1

[0029] 1) Hydrangea strain (Sparassiscrispa), preserved in China Agricultural Microorganism Strain Resource Bank, strain code ACCC51488. Seed the cultivated hydrangea strain seed liquid into the seed medium, the inoculation amount is 10%, and the seed medium is composed of starch 2.0%, glucose 0.5%, yeast extract 0.5%, peptone 0.3%, potassium dihydrogen phosphate 0.2%, Magnesium sulfate heptahydrate 0.2%, calcium sulfate 0.25%, soybean oil 0.02%; adjust the pH value to 6.0. The culture conditions are: the ventilation ratio is 1:0.5vvm, the tank pressure is 0.03Mpa, the culture temperature is 25-28°C, and the seed liquid can be obtained after culturing for about 12 days. Inoculate the cultivated seed liquid into 50L fermentation medium after sterilization, the inoculum size is 15%, and the fermentation medium is composed of starch 2.0%, glucose 0.5%, yeast extract 0.8%, peptone 0.5%, potassium dihydrogen phosphate 0.3%, seven Magnesium sulfate water 0.2%, calcium sulfate 0.25%...

Embodiment 2

[0037] 1) Hydrangea strain (Sparassiscrispa), preserved in China Agricultural Microorganism Strain Resource Bank, strain code ACCC51488. Seed the cultivated hydrangea strain seed liquid into the seed medium, the inoculation amount is 10%, and the seed medium is composed of starch 2.0%, glucose 0.6%, yeast extract 0.6%, peptone 0.3%, potassium dihydrogen phosphate 0.2%, Magnesium sulfate heptahydrate 0.2%, calcium sulfate 0.3%, soybean oil 0.02%; adjust the pH value to 6.0. The culture conditions are: the ventilation ratio is 1:0.5vvm, the tank pressure is 0.03Mpa, the culture temperature is 25-28°C, and the seed liquid can be obtained after culturing for about 12 days. Inoculate the cultivated seed solution into 50L fermentation medium after sterilization, the inoculation amount is 15%, and the fermentation medium is composed of starch 2.0%, glucose 0.8%, yeast extract 1.0%, peptone 0.5%, potassium dihydrogen phosphate 0.3%, seven Magnesium sulfate water 0.2%, calcium sulfate...

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Abstract

The invention discloses a quick deep-layer liquid state fermentation method for producing sparassis crispa bacterial powder. According to the method, the semi-continuous fermentation process is adopted to treat sparassis crispa strains to obtain fermentation liquor rich in abundant mycelia fast, and then the fermentation liquor is treated and separated through a micro-filtration membrane to obtain mycelia of sparassis crispa. The traditional intermittent sparassis crispa batch fermentation process is changed, and the mycelia are separated in the mode of efficient ceramic membrane micro-filtration instead of the separation mode of a centrifugal machine. The semi-continuous fermentation process overcomes the defects that the number of process links of traditional batch fermentation is large, middle clearance time is long, and equipment efficiency is low. The efficient fermentation liquor filtered through the ceramic membrane is adopted, the mycelia entering filter liquor can be reduced, the yield of the mycelia can be increased, COD of the filter liquor is reduced, the sewage handling capacity is reduced, damage to cells is avoided, and it is ensured that effective ingredients in the cells are recovered completely. Compared with the traditional technology, the method has remarkable economic benefits, social benefits and environment-friendly benefits.

Description

technical field [0001] The invention belongs to the technical field of microbes, and in particular relates to a method for rapidly producing fungal mycelium by deep liquid fermentation technology, more precisely, a method for rapidly producing hydrangea powder by deep liquid fermentation. Background technique [0002] Microbial fermentation can be divided into batch, fed-batch, semi-continuous, continuous and other modes. Batch fermentation consumes a lot of manpower and material resources. Each batch of fermentation needs to be loaded, sterilized, inoculated, discharged, and cleaned. The process is cumbersome, the fermentation cycle is long, and the production efficiency is low; Fed-batch fermentation Although the lack of nutrients or precursors can be supplemented by feeding, the product synthesis will inevitably be blocked due to the continuous accumulation of harmful metabolites; the production intensity of continuous fermentation is greatly improved compared with batch ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12R1/645
CPCC12N1/14
Inventor 王文风徐国华徐玲杨亚威张芙蓉孙妮娜董彩虹刘新利
Owner JIANGSU SHENHUA PHARMA
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