Quick deep-layer liquid state fermentation method for producing sparassis crispa bacterial powder
A technology of submerged liquid fermentation and Hydrangea, which is applied in the field of microorganisms, can solve the problems of heavy workload in the sewage treatment system, decline in product quality and yield, large consumption of manpower and material resources, etc., to improve equipment utilization, filtrate clarification, and installation The effect of easy maintenance
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Embodiment 1
[0029] 1) Hydrangea strain (Sparassiscrispa), preserved in China Agricultural Microorganism Strain Resource Bank, strain code ACCC51488. Seed the cultivated hydrangea strain seed liquid into the seed medium, the inoculation amount is 10%, and the seed medium is composed of starch 2.0%, glucose 0.5%, yeast extract 0.5%, peptone 0.3%, potassium dihydrogen phosphate 0.2%, Magnesium sulfate heptahydrate 0.2%, calcium sulfate 0.25%, soybean oil 0.02%; adjust the pH value to 6.0. The culture conditions are: the ventilation ratio is 1:0.5vvm, the tank pressure is 0.03Mpa, the culture temperature is 25-28°C, and the seed liquid can be obtained after culturing for about 12 days. Inoculate the cultivated seed liquid into 50L fermentation medium after sterilization, the inoculum size is 15%, and the fermentation medium is composed of starch 2.0%, glucose 0.5%, yeast extract 0.8%, peptone 0.5%, potassium dihydrogen phosphate 0.3%, seven Magnesium sulfate water 0.2%, calcium sulfate 0.25%...
Embodiment 2
[0037] 1) Hydrangea strain (Sparassiscrispa), preserved in China Agricultural Microorganism Strain Resource Bank, strain code ACCC51488. Seed the cultivated hydrangea strain seed liquid into the seed medium, the inoculation amount is 10%, and the seed medium is composed of starch 2.0%, glucose 0.6%, yeast extract 0.6%, peptone 0.3%, potassium dihydrogen phosphate 0.2%, Magnesium sulfate heptahydrate 0.2%, calcium sulfate 0.3%, soybean oil 0.02%; adjust the pH value to 6.0. The culture conditions are: the ventilation ratio is 1:0.5vvm, the tank pressure is 0.03Mpa, the culture temperature is 25-28°C, and the seed liquid can be obtained after culturing for about 12 days. Inoculate the cultivated seed solution into 50L fermentation medium after sterilization, the inoculation amount is 15%, and the fermentation medium is composed of starch 2.0%, glucose 0.8%, yeast extract 1.0%, peptone 0.5%, potassium dihydrogen phosphate 0.3%, seven Magnesium sulfate water 0.2%, calcium sulfate...
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