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Carbon nanotube photoacoustic contrast agent containing CLIC1 antibody and preparation method thereof

A technology of carbon nanotubes and photoacoustic imaging, applied in the biological field, can solve the problems of low dispersion and biological toxicity, and achieve the effect of good dispersion, strong stability and strong photoacoustic signal

Inactive Publication Date: 2016-05-18
XIN HUA HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved in the present invention is to provide a carbon nanotube photoacoustic contrast agent containing CLIC1 antibody and its preparation method in order to overcome the shortcomings of existing carbon nanotube materials with low dispersion and certain toxicity to organisms

Method used

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  • Carbon nanotube photoacoustic contrast agent containing CLIC1 antibody and preparation method thereof
  • Carbon nanotube photoacoustic contrast agent containing CLIC1 antibody and preparation method thereof
  • Carbon nanotube photoacoustic contrast agent containing CLIC1 antibody and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1 Preparation of carbon nanotube photoacoustic contrast agent

[0027] 1. Preparation of gold nanoparticles

[0028] (1) Preparation of branched polyethyleneimine (Mn=10000), chloroauric acid solution: 1.0754g of branched polyethyleneimine (PEI) was dissolved in 100mL of deionized water, sonicated and stirred to dissolve it; 1g Chlorauric acid was dissolved in 100mL deionized water and stored in cold storage as a mother liquor for later use.

[0029] (2) Take 1 mL of the above-mentioned mother liquor of chloroauric acid, add 50 mL of deionized water to dilute, and prepare a chloroauric acid solution with a concentration of 0.2 mg / mL.

[0030] (3) get 15mL chloroauric acid solution and add dropwise in the PEI solution of (1) preparation ( Mass ratio PEI:HAuCl 4 ＝300:1 ), avoid light and stir for 45 minutes to make it fully mixed.

[0031] (4) Preparation of nano-gold-branched polyethyleneimine solution (Au-PEI solution): the mixed solution of chloroauric ac...

Embodiment 2

[0045] Example 2 Preparation of carbon nanotube photoacoustic contrast agent

[0046] 1. Preparation of gold nanoparticles

[0047] (1) The specific method is the same as in Example 1.

[0048] (2) The specific method is the same as in Example 1.

[0049] (3) get 25mL of chloroauric acid and add in the prepared PEI solution ( Mass ratio PEI:HAuCl 4 ＝200:1 ), avoid light and stir for 45 minutes to make it fully mixed.

[0050] (4) Preparation of nano-gold solution: put the mixed solution of chloroauric acid and polyethyleneimine obtained in (3) in an oil bath at 90° C. for 1 hour. It was observed that the color of the solution was pink, and the average diameter of the obtained nano-gold particles detected by a transmission electron microscope was 25 nm, and the solution was allowed to stand to room temperature.

[0051] 2. Preparation of nano-gold-carbon nanotube composites

[0052] The specific method is the same as that in Example 1, and finally a carbon nanotube / nano...

Embodiment 3

[0053] Example 3 Preparation of carbon nanotube photoacoustic contrast agent

[0054] 1. Preparation of gold nanoparticles

[0055] (1) The specific method is the same as in Example 1.

[0056] (2) The specific method is the same as in Example 1.

[0057] (3) get 45mL of chloroauric acid and add in the prepared PEI solution ( Mass ratio PEI:HAuCl 4 ＝100:1 ), avoid light and stir for 45 minutes to make it fully mixed.

[0058] (4) Preparation of nano-gold solution: put the mixed solution of chloroauric acid and polyethyleneimine obtained in (3) in an oil bath at 90° C. for 1 hour. It was observed that the color of the solution was orange-red, and the average diameter of the obtained nano-gold particles detected by a transmission electron microscope was 20 nm. Let stand to room temperature.

[0059] 2. Preparation of nano-gold-carbon nanotube composites

[0060] The specific method is the same as that in Example 1, and finally a carbon nanotube / nano-gold composite photo...

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Abstract

The invention discloses a carbon nanotube photoacoustic contrast agent containing a CLIC1 antibody and a preparation method thereof. The photoacoustic contrast agent containing the CLIC1 antibody prepared by the method provided by the invention is a nano-composite material, is formed by covalent binding of carbon nanotubes (CNTs), a multi-amido compound, nanogold, thiolated polyethylene glycol (mPEG-SH) and an CLIC1 antibody. The preparation method provided by the invention has the advantages of convenient operation, green and environmental protection, etc. The carbon nanotube photoacoustic contrast agent containing the CLIC1 antibody provided by the invention has good dispersibility in water, strong stability, and good biological compatibility, can generate strong photoacoustic signasl, and has tumor cell targeting properties.

Description

technical field [0001] The invention relates to the biological field, in particular to a carbon nanotube photoacoustic contrast agent containing CLIC1 antibody and a preparation method thereof. Background technique [0002] Photoacoustic imaging is a new biomedical imaging method developed in recent years. When the pulsed laser is irradiated into the biological tissue, the light absorption domain of the tissue will generate a photoacoustic signal, and the photoacoustic signal of the biological tissue carries the light absorption characteristic information of the tissue, and the light absorption distribution in the tissue can be reconstructed by measuring the photoacoustic signal image. Photoacoustic imaging combines the advantages of pure optical imaging with high contrast and pure ultrasonic imaging with high penetration depth, avoids the influence of light scattering in principle, and breaks through the "soft limit" of high-resolution optical imaging depth (~1mm) , which...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K49/00
Inventor 刘颖斌谭庆刚陆巍李茂岚翁昊王宁束翌俊相闪闪谈竹君
Owner XIN HUA HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
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