An accelerant for inducing taxus chinensis to secrete paclitaxel

An accelerator, paclitaxel technology, applied in the field of cell engineering, can solve the problem of low secretion of paclitaxel, achieve the effect of improving the current situation of large-scale production and broad application prospects

Inactive Publication Date: 2016-05-04
天津市博爱生物药业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The present invention aims at the technical defects of the prior art, and provides an accelerator for inducing taxus to secrete paclitaxel, so as to solve the technical problem of low paclitaxel secretion in the process of taxus cell culture

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] An accelerator for inducing taxus to secrete paclitaxel, which consists of the following ingredients in parts by weight: 8 parts of potassium acetate, 4 parts of superphosphate, 2 parts of ferric ammonium citrate, 1 part of calcium nitrate, 1 part of potassium sodium tartrate, chloride 1 part copper, 1 part chrysin, 1 part ferric citrate pentahydrate, 1 part cobalt nitrate, 1 part glycine, 50 parts water.

Embodiment 2

[0019] An accelerator for inducing taxus to secrete paclitaxel, which consists of the following ingredients in parts by weight: 10 parts of potassium acetate, 6 parts of superphosphate, 3 parts of ferric ammonium citrate, 2 parts of calcium nitrate, 2 parts of potassium sodium tartrate, chloride 2 parts of copper, 2 parts of chrysin, 2 parts of ferric citrate pentahydrate, 2 parts of cobalt nitrate, 2 parts of glycine, 60 parts of water.

Embodiment 3

[0021] An accelerator for inducing taxus to secrete paclitaxel, which consists of the following ingredients in parts by weight: 9 parts of potassium acetate, 5 parts of superphosphate, 2.3 parts of ferric ammonium citrate, 1.8 parts of calcium nitrate, 1.5 parts of potassium sodium tartrate, chloride 1.6 parts of copper, 1.7 parts of chrysin, 1.8 parts of ferric citrate pentahydrate, 1.2 parts of cobalt nitrate, 1.8 parts of glycine, 55 parts of water.

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PUM

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Abstract

An accelerant for inducing taxus chinensis to secrete paclitaxel is provided. In preference, the accelerant comprises potassium acetate, calcium superphosphate, ammonium ferric citrate, calcium nitrate, potassium sodium tartrate tetrahydrate, copper chloride, chrysin, iron(III) citrate pentahydrate, cobalt nitrate, glycine, and other components. A weight ratio of the components is designed reasonably. A using method includes directly adding the accelerant into a cell culture liquid, wherein the accelerant can be added in an initial stage of culture and can be added when cells are in a stable culture stage. Experiments show that addition of the accelerant does not directly increase the cell culture density, but a paclitaxel yield in the cell culture liquid with same biomass is greatly increased, and therefore the accelerant has certain influences on cell metabolism characteristics of the taxus chinensis. Significant technical effects are achieved by innovative technical means. The accelerant is hoped to comprehensively improve large-scale production status of the paclitaxel at present and brings a wide application prospect.

Description

technical field [0001] The invention relates to the technical field of cell engineering, in particular to an accelerator for inducing taxus to secrete paclitaxel. Background technique [0002] Paclitaxel is a diterpenoid extracted from the bark of the Taxus genus Taxus. It is a new type of microtubule stabilizer with unique anticancer activity. It is considered by the National Cancer Institute of the United States to be the most important progress in tumor chemotherapy in the past 15 to 20 years. As a second-line treatment drug for advanced ovarian cancer, it has been approved for marketing in more than 40 countries so far, and has shown encouraging curative effects in the treatment of breast cancer, lung cancer, leukemia, gastrointestinal cancer and vascular restenosis after interventional therapy. . Paclitaxel has limited its clinical application due to the lack of resources and low water solubility. [0003] Paclitaxel and its analogues only exist in the genus Taxus an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P17/02
CPCC12P17/02
Inventor 夏雪城裘先临
Owner 天津市博爱生物药业有限公司
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