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Composition influencing plant cell metabolic regulation

A metabolic regulation and plant cell technology, applied in the field of compositions that affect plant cell metabolic regulation, can solve the problems affecting plant cell metabolic regulation and low paclitaxel secretion, and achieve the effect of improving the current situation of large-scale production and broad application prospects

Inactive Publication Date: 2016-03-23
天津市博爱生物药业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The present invention aims at the technical defects of the prior art, and provides a composition that affects the regulation of plant cell metabolism, so as to solve the technical problem of low paclitaxel secretion in the yew cell culture process

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] A composition affecting the regulation of plant cell metabolism, consisting of the following ingredients in parts by weight: 8 parts of arachidonic acid, 4 parts of sodium chloride, 2 parts of sodium nitrite, 1 part of indolebutyric acid, 6-benzyl gland 1 part of purine, 1 part of kinetin, 1 part of ethylenediaminetetraacetic acid, 1 part of zeatin, 1 part of calcium chloride, 1 part of ethyl acetate, 50 parts of water.

Embodiment 2

[0019] A composition affecting the regulation of plant cell metabolism, consisting of the following ingredients in parts by weight: 10 parts of arachidonic acid, 6 parts of sodium chloride, 3 parts of sodium nitrite, 2 parts of indolebutyric acid, 6-benzyl gland 2 parts of purine, 2 parts of kinetin, 2 parts of ethylenediaminetetraacetic acid, 2 parts of zeatin, 2 parts of calcium chloride, 2 parts of ethyl acetate, 60 parts of water.

Embodiment 3

[0021] A composition affecting the regulation of plant cell metabolism, consisting of the following ingredients in parts by weight: 9 parts of arachidonic acid, 5 parts of sodium chloride, 2.3 parts of sodium nitrite, 1.8 parts of indolebutyric acid, 6-benzyl gland 1.5 parts of purine, 1.6 parts of kinetin, 1.7 parts of ethylenediaminetetraacetic acid, 1.8 parts of zeatin, 1.2 parts of calcium chloride, 1.8 parts of ethyl acetate, 55 parts of water.

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Abstract

The invention provides a composition influencing plant cell metabolic regulation. According to the composition, such constituents as kinetin, sodium chloride, sodium nitrite, indolebutyric acid, 6-benzyladenine, ethylenediamine tetraacetic acid, zeatin, calcium chloride and ethyl acetate are selected preferentially, reasonable design is conducted on the using amount and the compounding ratio of the constituents, an application method of the composition is that the composition is directly added to cell culture fluid, and the composition can be added at the beginning culture stage and also can be added after cells enter the culture stable phase. It is found through experiments that after the composition is added, the cell culture density cannot be directly raised, but the paclitaxel productive rate in cell culture fluid of the same biomass is remarkably raised, and it is shown that the composition produces a certain influence on taxus chinensis cell metabolism characteristics. According to the composition influencing plant cell metabolic regulation, the prominent technical effect is achieved through the innovative technical means, by means of the composition, the large-scale production status of paclitaxel is expected to be upgraded comprehensively, and wide application prospect is brought about.

Description

technical field [0001] The invention relates to the technical field of cell engineering, in particular to a composition affecting the regulation and control of plant cell metabolism. Background technique [0002] Paclitaxel is a diterpenoid extracted from the bark of the Taxus genus Taxus. It is a new type of microtubule stabilizer with unique anticancer activity. It is considered by the National Cancer Institute of the United States to be the most important progress in tumor chemotherapy in the past 15 to 20 years. As a second-line treatment drug for advanced ovarian cancer, it has been approved for marketing in more than 40 countries so far, and has shown encouraging curative effects in the treatment of breast cancer, lung cancer, leukemia, gastrointestinal cancer and vascular restenosis after interventional therapy. . Paclitaxel has limited its clinical application due to the lack of resources and low water solubility. [0003] Paclitaxel and its analogues only exist i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/04C12P17/02
CPCC12N5/0025C12N2501/02C12N2501/16C12N2501/999C12P17/02
Inventor 夏雪城裘先临
Owner 天津市博爱生物药业有限公司
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