Low-cost extraction method for yeast lipid
An extraction method and low-cost technology, applied in the biological field, can solve the problems of cumbersome process, low oil extraction rate, and high cost, and achieve the effects of large sample processing volume, improved extraction efficiency, and low cost
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Examples
Embodiment 1
[0018] Select Lipozyme Starrii, after fermenting and cultivating to a stable stage, concentrate to obtain a yeast suspension containing 30wt% of yeast sludge, add 0.1% sodium dodecylbenzenesulfonate, 0.2% shell Polysaccharide-acetic acid solution, 0.1% 5'-phosphodiesterase crude enzyme solution, pH value adjusted to 3.0, stirred at 30° C. for 10 h to obtain a yeast dissolution solution. Add the extraction solvent hexane to the yeast enzymolysis solution at a volume ratio of 1:2, control the ultrasonic power to 100W, and the frequency to 20KHz. After ultrasonic oscillation for 5 minutes, centrifuge at 3000rpm for 5 minutes to collect the upper mixed oil. After the mixed oil is distilled, the yeast oil is obtained, and the extraction rate is 90.6%.
Embodiment 2
[0020] Select Lipozyme Starrii, after fermenting and cultivating to a stable stage, concentrate to obtain a yeast suspension containing 50wt% of yeast sludge, add 1% sodium dodecylbenzenesulfonate, 2% shell Polysaccharide-acetic acid solution, 0.5% 5'-phosphodiesterase crude enzyme solution, pH value adjusted to 7.5, stirred at 50° C. for 20 hours to obtain a yeast dissolution solution. Add the extraction solvent to the yeast enzymatic hydrolysis solution at a volume ratio of 1:5, mix equal volumes of hexane and ethanol, control the ultrasonic power to 300W, and the frequency to 40KHz. After ultrasonic oscillation for 15 minutes, centrifuge at 5000rpm for 10 minutes to collect the mixed oil in the upper layer. After the mixed oil is distilled, the yeast oil is obtained, and the extraction rate is 96.3%.
Embodiment 3
[0022] Select Lipozyme Starrii, after fermenting and cultivating to a stable stage, concentrate to obtain a yeast suspension containing 40wt% of yeast sludge, add 0.5% sodium dodecylbenzenesulfonate, 1% shell Polysaccharide-acetic acid solution, 0.3% 5'-phosphodiesterase crude enzyme solution, pH value adjusted to 5.0, stirred at 40° C. for 10 h to obtain a yeast dissolution solution. Add the extraction solvent to the yeast enzymolysis solution according to the volume ratio of 1:3, mix the hexane and petroleum ether in equal volumes, control the ultrasonic power to 200W, and the frequency to 30KHz. After the ultrasonic oscillation is completed for 10 minutes, centrifuge at 4000rpm for 8 minutes to collect the upper layer of mixed oil. . After the mixed oil is distilled, the yeast oil is obtained, and the extraction rate is 94.1%.
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com