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A kind of method for making permanent glass slide specimen of bryophytes

A technology for making bryophytes and specimens, which is applied in botany equipment and methods, plant preservation, application, etc. It can solve the problems of many chemical reagents, affecting observation and specimen protection, and long storage time, so as to achieve clear and transparent cell tissue, Good water-locking and moisturizing performance, long-term storage effect

Inactive Publication Date: 2018-02-09
SHANGHAI NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] In natural science research, bryophyte species identification, biological experiment teaching and extracurricular scientific and technological activities, especially in the bryophyte species identification and morphological research, it is necessary to make a large number of moss microscopic slide specimens; moss slides can be divided into temporary glass slides. At present, the production of temporary slides generally uses water directly as a floating agent, and then directly observes after covering with a cover glass. However, the temporary slides made in this way can only be stored for a short period of time, and the water will volatilize after a long time , affect further observation, and cannot be further sealed for storage; permanent glass slides require repeated observation and long-term storage, so the mounting medium used is required to be stored for a long time, not deteriorated, has strong adhesion, and the refractive index is the same as that used for slides, The refractive index of the cover glass etc. is similar
At present, paraffin-wax technology is usually used for sealing, but the process of paraffin-wax technology is cumbersome, the production time is long, and it is easy to be restricted by conditions. Many chemical reagents are used in the production process, and some reagents are poisonous to humans. It becomes brittle, hard or deformed, which affects the accuracy of observation, and because the water content in the cells of bryophytes is high, the tissue is easy to shrink and deform during the dehydration process, so it is not suitable for bryophytes
[0003] Chinese patent CN 101571626A discloses a method for transparent sealing of biological microscopic slide specimens, using novolac as a mounting agent to seal the specimens, and the method uses varnish for sealing with high light transmittance, high quality and low price, and easy However, the phenolic varnish is brittle after drying, and it is easy to turn yellow under the action of ultraviolet light, so it will affect the follow-up observation and specimen protection

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] (1) 30g of gum arabic, 50ml of double distilled water, 20ml of glycerin, and 180g of chloral hydrate were mixed and stirred at room temperature to form a liquid mounting medium;

[0021] (2) Soak the leaf or its plant in double-distilled water, place it with tweezers in the center of a glass slide dripped with a drop of double-distilled water, observe and adjust its shape under a 10-fold microscope;

[0022] (3) Use filter paper to absorb the double-distilled water beyond 1 mm around the specimen, so as to keep the moss specimen moist around and not have too much double-distilled water;

[0023] (4) Use a glass rod to dip 1-2 drops of mounting agent onto the moss specimen, cover with a cover glass without air bubbles, and place it at room temperature for a week until the mounting agent is dry and the specimen cannot be moved. As soon as the slide is affixed with a label, indicating the name of the specimen, the place where the specimen was taken, and the time of product...

Embodiment 2

[0026] (1), 30g of gum arabic, 50ml of double distilled water, 20ml of glycerin, and 180g of chloral hydrate were mixed and stirred at room temperature to form a liquid mounting medium;

[0027] (2) Put the moss moss leaf or its plant in double-distilled water for infiltration, place it with tweezers in the center of a glass slide dripped with a drop of double-distilled water, observe and adjust its shape under a 10-fold microscope;

[0028] (3) Use filter paper to absorb the double-distilled water beyond 1 mm around the specimen, so as to keep the moss specimen moist and not have too much double-distilled water;

[0029] (4) Use a glass rod to dip 1-2 drops of mounting agent onto the moss specimen, cover with a cover glass without air bubbles, and place it at room temperature for one week until the mounting agent dries and the specimen cannot After moving, affix a label on the slide, indicating the name of the specimen, the place where the specimen was taken, and the time of ...

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PUM

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Abstract

The invention relates to a method for making a permanent glass slide specimen of bryophyte. Gum Arabic, double distilled water, glycerin and chloral hydrate are mixed with stirring at normal temperature, and a liquid mounting medium is prepared; moss leaves or plants are placed in the double distilled water, after infiltration, the moss leaves or plants are placed in the center of a slide glass with a droplet of double distilled water, and the moss leaves or plants are observed under a 10x microscope for adjusting shapes; a piece of filter paper is used for absorbing double distilled water which is 1 millimeter or more far away from the specimen, till the circumference of the moss specimen is moistened without excessive double distilled water; a glass rod is used for dipping 1-2 droplets of the mounting medium, and the droplets are dropped on the moss specimen, a piece of cover glass is covered without bubbles contained, the specimen is placed at normal temperature till the mounting medium is dried and can fix the specimen without movement, a label is plastered on the slide glass, and the permanent glass slide specimen of bryophyte is prepared. Compared with prior art, the produced specimen has the advantages of good transparency, simple method, long preservation time limit, and good preservation effect of original state of the specimen.

Description

technical field [0001] The invention relates to a method for making a biological permanent glass slide, in particular to a method for making a bryophyte permanent glass slide specimen. Background technique [0002] In natural science research, bryophyte species identification, biological experiment teaching and extracurricular scientific and technological activities, especially in the bryophyte species identification and morphological research, it is necessary to make a large number of moss microscopic slide specimens; moss slides can be divided into temporary glass slides. At present, the production of temporary slides generally uses water directly as a floating agent, and then directly observes after covering with a cover glass. However, the temporary slides made in this way can only be stored for a short period of time, and the water will volatilize after a long time , affect further observation, and cannot be further sealed for storage; permanent glass slides require rep...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N3/00
CPCA01N3/00
Inventor 吴倩倩郭水良于晶
Owner SHANGHAI NORMAL UNIVERSITY
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