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Laser pump time resolution up-conversion luminescence living imaging system

A time-resolved, laser-pumped technology, applied in the field of fluorescence imaging, can solve the problems that restrict fluorescence imaging technology, achieve the effect of improving accuracy and sensitivity, and avoiding interference

Active Publication Date: 2016-04-27
FUDAN UNIV
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Problems solved by technology

At present, time-resolved fluorescence technology has been applied in fluorescence spectrum detection and fluorescence confocal microscopy, but there are few applications in small animal in vivo fluorescence imaging systems, so if time-resolved technology can be combined with up-conversion fluorescence in vivo imaging technology, The development of a new type of time-resolved up-conversion luminescence in vivo imaging system is expected to solve the bottleneck problem that restricts the clinical application of fluorescence imaging technology, and provide a powerful tool for life science and medical research

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  • Laser pump time resolution up-conversion luminescence living imaging system
  • Laser pump time resolution up-conversion luminescence living imaging system
  • Laser pump time resolution up-conversion luminescence living imaging system

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Embodiment 1

[0020] Example 1: In vivo imaging of mice labeled with upconversion fluorescent nanomaterials using a laser-pumped time-resolved upconversion luminescence in vivo imaging system.

[0021] The structure of the imaging system is as follows figure 1 As shown, it consists of a semiconductor laser 1, a pulse controller 2, an optical chopper 3, a CCD detector 4, an optical lens 5, a sample stage 6 and a computer 7; wherein, the semiconductor laser 1 has a TTL modulation function as a time-resolved Imaging fluorescent excitation source, its outgoing light is aimed at the sample stage 6; the sample stage 6 is used to place the up-conversion fluorescent probe material and the cells, living animals and tissues labeled by the up-conversion fluorescent probe material, and the sample stage 6 is aligned with the optical lens 5 The optical lens 5 is aimed at the optical chopper 3, and the optical focus of the optical lens 5 is in the same plane with the optical chopper 3; the optical chopper...

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Abstract

The invention belongs to the technical field of fluorescence imaging, in particular to a laser pump time resolution up-conversion luminescence living imaging system, consisting of a semiconductor laser, a pulse emitter, an optical chopper, an optical camera, a CCD detector and a computer; according to the system, by using the feature of a long luminescence service life of an up-conversion luminescence material, the up-conversion luminescence material is excited by short pulse light, delay fluorescence time periods without existence of excited light are collected, thus realizing real-time, non-invasive, high signal to noise ratio and high sensitivity imaging on a material, a cell or biological tissue marked with an up-conversion luminescence probe. In addition, by using pulse exciting light, the total amount of laser irradiation can be greatly reduced to further reduce the influence on the biological tissue. Signal interference of exciting light in fluorescence imaging is effectively removed, imaging sensitivity and accuracy of fluorescence detection are greatly improved, and a powerful tool is provided for the living optical detection in material science, bioscience and medical research.

Description

technical field [0001] The invention belongs to the technical field of fluorescence imaging, and in particular relates to a laser-pumped time-resolved up-conversion luminescent living body imaging system. Background technique [0002] Fluorescence imaging, as an emerging biological imaging technology, has the advantages of relatively low cost, fast imaging speed, high sensitivity, and low invasiveness. In addition, unlike clinical imaging methods such as X-ray computed tomography, magnetic resonance imaging, single photon computed tomography and other imaging methods, fluorescence imaging can protect the detected organisms from radiation damage. Therefore, fluorescence imaging technology has broad application prospects in the field of biomedicine. The realization of fluorescence imaging is based on the fact that materials with photoluminescent properties produce fluorescence emission under the excitation of external light sources, and the specific physiological process of t...

Claims

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Application Information

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IPC IPC(8): G01N21/64
CPCG01N21/6402Y02A40/70
Inventor 李富友冯玮朱幸俊
Owner FUDAN UNIV
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