Extraction method of bovine tendon collagen

Abstract

The invention provides an extraction method of bovine tendon collagen. The extraction method has the advantages of high yield, short production period and high product purity, and comprises the following steps of: preprocessing bovine tendon, and freezing and crushing the bovine tendon; putting the crushed bovine tendon into a Tris-HCL buffer solution; then, adding cold-adapted protease MCP-01 accounting for 0.1 percent of the weight of the bovine tendon into the buffer solution; performing enzymolysis at 40 DEG C; then, obtaining an enzymolysis solution; centrifuging the enzymolysis solution; taking precipitates; dissolving the precipitates by 0.1-percent acetic acid; performing filtration to obtain filter liquid; salting out the filter liquid to obtain a salting-out solution; performing centrifugation twice; adding water for dissolution; then, adding 0.1-percent acetic acid for continuous dissolution; and performing freeze drying to obtain the bovine tendon collagen. The extraction method has the beneficial effects that the cold-adapted protease MCP-01 is used for hydrolyzing the bovine tendon in a buffer liquid system, so that collagen helix molecules of collagen micro fiber are exposed; collagen filaments expand and loose, so that the hydrolysis is fast and sufficient; and the yield and the product purity are further improved.

Description

technical field [0001] The invention relates to the field of medical bioengineering, in particular to a method for extracting bovine tendon collagen. Background technique [0002] Collagen is a biological polymer substance, a white, opaque, unbranched fibrous protein, mainly distributed in the connective tissue of mammals, and it is harmful to animal and human skin, blood vessels, bones, tendons, etc. Tendons, teeth, and cartilage are all important in the formation of these connective tissues. [0003] Most collagen is insoluble in water in its natural state and exists in the form of collagen fibrils or collagen fibers in the body. The basic structural unit of collagen fibrils is the procollagen molecule, and the peptide chains (subunits) are intertwined into a unique three-strand right-handed helical structure. Because the above structure is very stable, collagen is difficult to be biologically degraded, and only a few proteases can hydrolyze collagen fibers under specifi...

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Problems solved by technology

[0006] However, the above-mentioned method does not disclose specific process parameters, such as what kind of enzyme treatment and treatment time, and the existing preparation method has the following defects: trypsin, papain and pepsin are used in the enzymolysis process, and the reaction time is long. The yield is low, the purity is low, and the produced collagen material has defects in mechanical properties, biological stability, etc., which limit its practical application

In order to overcome the above shortcomings, the high voltage pulse method is also used to extract, but still can not achieve satisfactory results

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