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Fermentation technology for industrially producing L-hydroxyproline through fermentation method

A technology of hydroxyproline and fermentation technology, applied in the field of microbial fermentation technology, can solve the problems of L-hydroxyproline fermentation level, production cost three waste discharge and the like, and achieves the advantages of reducing production cost, avoiding three waste pollution and improving fermentation level. Effect

Inactive Publication Date: 2016-04-20
TIANJIN JINGYE FINE CHEM
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] At present, the research on the production of L-hydroxyproline by microbial fermentation is still in the laboratory stage, and a mature industrial production process has not yet been formed. However, the advanced industrial production process, especially the fermentation process, has a great impact on the fermentation level of L-hydroxyproline, Production cost and three waste emissions have a crucial impact

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0016] The fermentation process of industrial production of L-hydroxyproline by fermentation method, firstly, the strains are cultivated in liquid medium to obtain mature seeds, and then transferred to the fermentation medium to ferment and metabolize to produce L-hydroxyproline;

[0017] Among them, the liquid seed medium aqueous solution formula (g / L) is: glucose 40, yeast powder 7.5, ammonium sulfate 2.0, potassium dihydrogen phosphate 7.5, potassium chloride 1.9, FeSO 4 ·7H 2 O0.28, MgSO 4 ·7H 2 O0.32, trace elements (ppm): MnSO 4 ·H 2 O0.5, CoCl 2 ·6H 2 O1.4, NiSO 4 ·6H 2 O1.2, CuSO 4 ·7H 2 O0.5, ZnSO 4 ·7H 2 O0.6, VB120, defoamer 100, AMP50.

[0018] The fermentation medium formula (g / L) is as follows: glucose 20, yeast powder 5.0, ammonium sulfate 10, potassium dihydrogen phosphate 5.0, potassium chloride 1.9, MgSO 4 ·7H 2 O0.32, FeSO 4 ·7H 2 O0.75, trace elements (ppm): MnSO 4 ·H 2 O1.0, CoCl 2 ·6H 2 O1.4, NiSO 4 ·6H 2 O1.4, CuSO 4 ·7H 2 O1.0, Z...

example 2

[0023] The fermentation process of industrial production of L-hydroxyproline by fermentation method, firstly, the strains are cultivated in a liquid medium to obtain mature seeds, and then transferred to the fermentation medium to ferment and metabolize to produce L-hydroxyproline;

[0024] Among them, the liquid seed medium aqueous solution formula (g / L) is: glucose 40, yeast powder 7.5, ammonium sulfate 2.0, potassium dihydrogen phosphate 7.5, potassium chloride 1.9, FeSO 4 ·7H 2 O0.28, MgSO 4 ·7H 2 O0.32, trace elements (ppm): MnSO 4 ·H 2 O0.5, CoCl 2 ·6H 2 O1.4, NiSO 4 ·6H 2 O1.2, CuSO 4 ·7H 2 O0.5, ZnSO 4 ·7H 2 O0.6, VB120, defoamer 100, AMP50.

[0025] The fermentation medium formula (g / L) is as follows: glucose 20, yeast powder 5.0, ammonium sulfate 10, potassium dihydrogen phosphate 5.0, potassium chloride 1.9, MgSO 4 ·7H 2 O0.32, FeSO 4 ·7H 2 O0.75, trace elements (ppm): MnSO 4 ·H 2 O1.0, CoCl 2 ·6H 2 O1.4, NiSO 4 ·6H 2 O1.4, CuSO 4 ·7H 2 O1.0,...

example 3

[0030] The fermentation process of industrial production of L-hydroxyproline by fermentation method, firstly, the strains are cultivated in a liquid medium to obtain mature seeds, and then transferred to the fermentation medium to ferment and metabolize to produce L-hydroxyproline;

[0031] Among them, the liquid seed medium aqueous solution formula (g / L) is: glucose 40, yeast powder 7.5, ammonium sulfate 2.0, potassium dihydrogen phosphate 7.5, potassium chloride 1.9, FeSO 4 ·7H 2 O0.28, MgSO 4 ·7H 2 O0.32, trace elements (ppm): MnSO 4 ·H 2 O0.5, CoCl 2 ·6H 2 O1.4, NiSO 4 ·6H 2 O1.2, CuSO 4 ·7H 2 O0.5, ZnSO 4 ·7H 2 O0.6, VB120, defoamer 100, AMP50.

[0032] The fermentation medium formula (g / L) is as follows: glucose 20, yeast powder 5.0, ammonium sulfate 10, potassium dihydrogen phosphate 5.0, potassium chloride 1.9, MgSO 4 ·7H 2 O0.32, FeSO 4 ·7H 2 O0.75, trace elements (ppm): MnSO 4 ·H 2 O1.0, CoCl 2 ·6H 2 O1.4, NiSO 4 ·6H 2 O1.4, CuSO 4 ·7H 2 O1.0,...

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Abstract

Provided is a fermentation technology for industrially producing L-hydroxyproline through a fermentation method. The fermentation technology comprises the following steps that 1, a strain is cultured in a liquid culture medium, and a mature seed is obtained; 2, the mature seed is moved into the fermentation culture medium in a fermentation tank for fermentation and metabolism, and the L-hydroxyproline is produced. The fermentation technology is applied to production of the L-hydroxyproline through microbial fermentation, the fermentation level is improved, the production cost is reduced, and three-waste pollution is avoided.

Description

technical field [0001] The invention relates to a microbial fermentation process, in particular to a fermentation process for the industrial production of L-hydroxyproline by a fermentation method. Background technique [0002] Hydroxyproline (Hyp) is an imino acid, which is the product of L-proline hydroxylation, and its molecular formula is C 5 h 9 NO 3 . Hydroxyproline is white flaky crystal or crystalline powder, slightly sweet, with a melting point of 274°C, easily soluble in water and slightly soluble in ethanol. Trans-4-hydroxyproline is more common in nature, and trans-4-hydroxyproline was first found in animal collagen. Trans-4-hydroxyproline is widely used in medicine, chemical industry, animal feed, nutrition and beauty industry, etc. [0003] At present, the methods for producing hydroxyproline reported at home and abroad mainly include proteolytic extraction, chemical synthesis, microbial fermentation and enzymatic conversion. Among them, proteolytic extrac...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P13/24
CPCC12P13/24
Inventor 林俊辉赵体金彭久合
Owner TIANJIN JINGYE FINE CHEM
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