A method for dissolving medium and low-grade phosphate rocks by using microbial flora in phosphate rock pit water
A technology of microbial flora and mine water, applied in the field of phosphate rock mining and processing, can solve the problems of rare utilization of low-grade phosphate rock and single, and achieve the effects of low requirements for cultivation conditions, simple process and simplified process
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Embodiment 1
[0021] A method for dissolving medium and low-grade phosphate rocks by utilizing microbial flora in phosphate rock pit water, comprising the steps of:
[0022] 1) Enrichment culture of microbial flora in phosphate rock pit water: put phosphorus-containing medium and phosphate rock pit water in a volume ratio of 1:0.8 into a container (such as a triangular flask), and place the container in a constant temperature shaker at 22°C Vibrating at a rotational speed of 140 rpm for 4 days, the phosphate rock mine water microbial flora enrichment culture solution A of the first enrichment culture was obtained.
[0023] Put the phosphorus-containing medium and the phosphate mine water microbial flora enrichment culture solution A of the first enrichment culture into a container at a volume ratio of 1:0.7, and place the container in a constant temperature shaker at 30°C at 100 rpm Shake culture at a rotation speed of 5 minutes for 5 days to obtain the phosphate mine pit water microbial fl...
Embodiment 2
[0029] A method for dissolving medium and low-grade phosphate rocks by utilizing microbial flora in phosphate rock pit water, comprising the steps of:
[0030] 1) Enrichment culture of microbial flora in phosphate rock pit water: put phosphorus-containing medium and phosphate rock pit water in a volume ratio of 1:0.7 into a container (such as a triangular flask), and place the container in a constant temperature shaker at 25°C Vibrating culture at a rotational speed of 120 rpm for 4 days to obtain the phosphate mine pit water microbial flora enrichment culture solution A for the first enrichment culture.
[0031] Put the phosphorus-containing medium and the phosphate mine water microbial flora enrichment culture solution A of the first enrichment culture into a container at a volume ratio of 1:0.7, and place the container in a constant temperature shaker at 25°C at 120 rpm. Shaking culture at a rotational speed of 10 minutes for 4 days to obtain the phosphate mine pit water mi...
Embodiment 3
[0037] A method for dissolving medium and low-grade phosphate rocks by utilizing microbial flora in phosphate rock pit water, comprising the steps of:
[0038] 1) Enrichment culture of microbial flora in phosphate rock pit water: put phosphorus-containing medium and phosphate rock pit water in a volume ratio of 1:0.5 into a container (such as a triangular flask), and place the container in a constant temperature shaker at 25°C Vibrate culture at a rotational speed of 160 rpm for 3 days to obtain the phosphate mine pit water microbial flora enrichment culture solution A for the first enrichment culture.
[0039] Put the phosphorus-containing medium and the phosphate mine water microbial flora enrichment culture solution A of the first enrichment culture into a container at a volume ratio of 1:0.6, and place the container in a constant temperature shaker at 30°C at 120 rpm. Shake culture at a rotation speed of 5 minutes for 5 days to obtain the phosphate mine pit water microbial...
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