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Preparation used for restraining enterovirus infection

An enterovirus and preparation technology, applied in the field of preparations for inhibiting enterovirus infection, can solve the problems of high morbidity, heavy burden on families and society, and achieve the effect of inhibiting infection

Inactive Publication Date: 2016-04-20
INST OF PLA FOR DISEASE CONTROL & PREVENTION
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

EV71 virus has the highest incidence rate in preschool children, especially in the age group under 5 years old. In addition to causing the general clinical symptoms of hand, foot and mouth disease, it can also cause aseptic meningitis, brainstem encephalitis and polio-like paralysis. A neurological disease that places a heavy burden on families and society

Method used

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  • Preparation used for restraining enterovirus infection
  • Preparation used for restraining enterovirus infection
  • Preparation used for restraining enterovirus infection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Embodiment 1, the preparation of plant extract compound formula virus infection inhibitor

[0038] Epigallocatechin gallate (EGCG), tannic acid and astragalus polysaccharide are uniformly mixed according to a mass ratio of 1:1:1.5 to obtain a plant extract compound formulation.

[0039]Before the experiment, it was dissolved in PBS buffer, made into 2560 μg / ml (the total concentration of EGCG, tannic acid and astragalus polysaccharide in the solution), filtered, and after sterilization, it was stored at -20°C for later use.

Embodiment 2

[0040] Embodiment 2, cytotoxicity test

[0041] In this example, the neutral red phagocytosis method was used to measure the cytotoxicity of the compound formulation prepared in Example 1 to mammalian cells. The specific operation is as follows:

[0042] The compound formulation preparation solution (2560 μg / ml) prepared in Example 1 was diluted step by step to obtain a total of 6 concentration. Then the dilutions of different dilutions were added to the 96-well cell culture plate with Vero cells and the cell density was about 80%, 100 μl in each well, and 4 duplicate holes were made for each dilution. Add compound formula preparation) as a control, after 2 hours of action, discard the test solution, add cell maintenance culture medium, add 200 μ l to each well, place it in a cell incubator and cultivate, after 48 hours, add 0.1% (0.1g / 100mL) to each well Neutral red 25μl, after reacting at 37°C for 1.5h, suck out the liquid in each well and rinse twice with PBS. Add 100 μ...

Embodiment 3

[0048] Embodiment 3, enterovirus infection inhibition test

[0049] In this example, the CPE method was used to measure the inhibitory effect of the compound formulation prepared in Example 1 on enterovirus infection. The enterovirus tested was enterovirus EV71-Hn2 strain.

[0050] Take a 96-well culture plate cultured with Vero cells that has grown into a single layer of cells with a growth density of about 80%, pour off the culture medium, rinse the cells with PBS for 3 times, and add them under the three conditions of A, B, and C respectively. The compound formula preparation that example 1 prepares:

[0051] A. Simultaneously with virus adsorption: put an equal volume of 2×100TCID 50 After mixing the enterovirus virus solution with 2 times the concentration of the test substance solution, add 100 μl / well of the mixed solution to the cell culture plate, place it in the cell culture incubator, and discard it after the virus is adsorbed for 1 hour. After washing the cell s...

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Abstract

The invention discloses a preparation used for restraining enterovirus infection. The preparation is used for preparing products used for restraining enterovirus infection, and used for restraining enterovirus infection. The preparation is mainly prepared by mixing epigallocatechin gallate, tannin and astragalus polysaccharides. The mass ratio of epigallocatechin gallate to tannin to astragalus polysaccharides is (0.5-1.0):(0.5-1.0):(0.5-1.5). Cytotoxicity of the complex formula preparation is not higher than that of a control sample ribavirin obtaining security permission, and the preparation is safe. With the safe use concentration, the preparation is used preventively to restrain enterovirus infection effectively, and has commercial value of being further developed into an enterovirus infection inhibitor.

Description

technical field [0001] The invention belongs to the field of biotechnology and relates to a preparation for inhibiting enterovirus infection. Background technique [0002] Since the discovery of EV71 in the 1970s, there have been 3 large-scale HFMD epidemics caused by EV71, and there have been fatal cases each time. EV71 virus has the highest incidence rate in preschool children, especially in the age group under 5 years old. In addition to causing the general clinical symptoms of hand, foot and mouth disease, it can also cause aseptic meningitis, brainstem encephalitis and polio-like paralysis. It is a neurological disease that imposes a heavy burden on families and society. [0003] Plant compounds are called "the seventh type of nutrients", which widely exist in daily food and are a class of non-nutritive chemical substances that have special effects on human health. Relevant studies have found that some plant compounds have the biological activity of inhibiting virus i...

Claims

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Application Information

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IPC IPC(8): A61K31/715A61P1/00A61P31/14A61K31/7024A61K31/353
CPCA61K31/353A61K31/7024A61K31/715A61K2300/00
Inventor 常国辉刘京梅杨益黄留玉罗丽晓李瑾惠罗彦军孙走南唐玥苏文莉张洁刘璇
Owner INST OF PLA FOR DISEASE CONTROL & PREVENTION
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