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Application of sophora tonkinensis endophytic fungus GRPH-0 in prevention and treatment of Candida albicans

A technology for endophytic fungi and lacquer spot bacteria, applied in the field of microorganisms, can solve problems such as difficulties, and achieve the effects of low cost and simple and easy method.

Active Publication Date: 2016-04-13
GUANGXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the drugs commonly used in clinical treatment of Candida albicans infection mainly include azoles such as fluconazole, polyenes such as amphotericin B and pyrimidines such as 5-fluorocytosine, but with the wide application of these antifungal drugs, leading to The emergence of a large number of drug-resistant strains, therefore, Candida albicans infection has become an increasingly serious problem, bringing great difficulties and challenges to clinical treatment, and there is an urgent need to develop new antifungal drugs

Method used

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  • Application of sophora tonkinensis endophytic fungus GRPH-0 in prevention and treatment of Candida albicans
  • Application of sophora tonkinensis endophytic fungus GRPH-0 in prevention and treatment of Candida albicans
  • Application of sophora tonkinensis endophytic fungus GRPH-0 in prevention and treatment of Candida albicans

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Experimental program
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Effect test

Embodiment 1

[0024] Isolation, screening and identification of strains

[0025] 1. Isolation of strains

[0026] Materials for testing: cultivated Sophora japonica collected from the herbarium of Guangxi University.

[0027] Medium: PDA medium: 1000ml medium contains 300g of potato, 20g of glucose, 20g of agar, 0.1g of chloramphenicol, pH of the medium is 6.0±0.2, and sterilized by 121°C damp heat for 25min.

[0028] Surface disinfection: Rinse the fresh and healthy Vietnamese Sophorae root with a length of 6-8cm and a width of 1-2cm with running water for 30 minutes to wash away the sediment, then rinse the washed Vietnamese Sophorae root with sterile water twice, and air-dry the surface moisture , moved to the ultra-clean workbench, and under aseptic conditions, the Sophora japonica root was soaked in 75% ethanol for 1 min, rinsed with sterile water twice, soaked in sodium hypochlorite (available chlorine 1%) for 2 min, washed with sterile water for 3 times , dry the surface with steri...

Embodiment 2

[0081] Inhibitory effect of metabolites of strains on Candida albicans

[0082] 1. Fermentation of strains and extraction of metabolites

[0083] (1) Inoculate the endophytic fungus GRPH-0 of Sophora japonica in PDA medium, and place it at a temperature of 28°C for 20 days. The resulting culture material is cut into small pieces and transferred to a sterile solid medium (400g potato, 20g Dextrose and 20g sucrose) in a 2-liter Erlenmeyer flask, placed at 28°C for fermentation for 30 days;

[0084] (2) After the fermentation is completed, soak the fermented product with 2 times the methanol of the fermented product and ultrasonically for 40 minutes, then filter it with gauze;

[0085] (3) Repeat step (2) twice, combine the two filtrates and concentrate under reduced pressure to form an extract, and obtain the methanol crude extract of the endophytic fungus GRPH-0, which is the metabolite of the endophytic fungus GRPH-0 .

[0086] 2. Minimum inhibitory concentration of GRPH-0 ...

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Abstract

The invention discloses sophora tonkinensis endophytic fungus GRPH-0. The sophora tonkinensis endophytic fungus GRPH-0 has the type name of Myrothecium sp. GRPH-0, an ITS sequence of the Myrothecium sp. GRPH-0 is as shown in SEQ ID NO. 1, and the Myrothecium sp. GRPH-0 is collected by the General Microorganisms Center of China Committee for Culture Collection of Microorganisms, which is located at Institute of Microbiology, Chinese Academy of Sciences, 3#, 1# Courtyard, Beichen West Road, Chaoyang District, Beijing, on September 29, 2015 and has the collection number of CGMCC No. 11391. According to the sophora tonkinensis endophytic fungus GRPH-0, the Myrothecium sp. GRPH-0 has a very remarkable inhibiting action on the Candida albicans; a method for preparing metabolites of the endophytic fungus Myrothecium sp. GRPH-0 is easy and feasible and is low in cost.

Description

technical field [0001] The invention belongs to the technical field of microbes, and in particular relates to the application of an endophytic fungus GRPH-0 in the prevention and treatment of Candida albicans. Background technique [0002] Candida albicans is a common opportunistic pathogenic fungus in the human body. When the body's immunity is weakened or the immune system is damaged, it will reproduce in large numbers and cause disease. In the past 20 years, due to the clinical use of anti-tumor drugs, antibacterial agents and immunosuppressants for tumor patients and organ transplant patients, as well as the prevalence of chronic wasting diseases and AIDS, the incidence of Candida albicans infection is higher than that of immunocompromised patients. sharply increased in the population. Candida albicans infection has increasingly become a common disease, frequently-occurring disease, and the mortality rate is increasing year by year. At present, the drugs commonly used ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12P1/02A61K36/06A61P31/10C12R1/645
CPCA61K36/06C12P1/02C12N1/145C12R2001/645
Inventor 姚裕群吴炫柯黄荣韶李良波
Owner GUANGXI UNIV
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