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A composite nano-antibacterial material for the treatment of vancomycin-resistant pathogens

An antibacterial material, vancomycin technology, applied in antibacterial drugs, preparations for in vivo experiments, wave energy or particle radiation treatment materials, etc., can solve the problem of low excitation efficiency of near-infrared light sources and poor fluorescence performance of porphyrin molecules. In order to improve water solubility and biocompatibility, it is not easy to interfere with sample autofluorescence, and the spectral bandwidth is narrow.

Inactive Publication Date: 2018-10-12
LUDONG UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are still some problems with photodynamic therapy as a new solution for the antibacterial treatment of VRE pathogenic bacteria. For example, how to ensure that the photosensitizer is accurately targeted to the pathogenic bacteria? How to accurately locate the phototherapy area? In order to solve these problems, the researchers designed and synthesized a porphyrin-linked vancomycin dimer, and used the enhanced specific affinity of the dimer to the surface of VRE bacteria to target and immobilize the photosensitive molecule (porphyrin) on the bacterial surface. , localization and photodynamic therapy with the help of porphyrin's own fluorescent signal
However, the porphyrin-linked vancomycin dimer as a targeted photosensitive drug still has the following defects, which need further improvement: (1) The dimer synthesis reaction is time-consuming, low yield, and the purification process is complicated and costly; (2) The biocompatibility of the dimer is poor, and it is easy to combine with serum protein to form a precipitate, which cannot be used for the treatment of infection in deep tissues in the body; (3) The fluorescence performance of the porphyrin molecule is not good, and the excitation efficiency of its near-infrared light source is low. Not suitable for in vivo visualization therapy through skin garments and tissues

Method used

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  • A composite nano-antibacterial material for the treatment of vancomycin-resistant pathogens
  • A composite nano-antibacterial material for the treatment of vancomycin-resistant pathogens
  • A composite nano-antibacterial material for the treatment of vancomycin-resistant pathogens

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] A kind of preparation process of composite nano antibacterial material is as follows:

[0033] (1) Synthesis of vancomycin-porphyrin monomer:

[0034] Dissolve 52.8 mg of vancomycin and 20.8 mg of porphyrin in 2 mL of dry dimethyl sulfoxide, stir in an ice bath for 10 min, then add 38.0 mg of O-benzotriazepam dissolved in 1 mL of N,N-dimethylformamide Azole-tetramethyluronium hexafluorophosphate and 0.06 mL of N,N-diisopropylethylamine. The ice bath was removed, and the reaction was stirred overnight. At the end of the reaction, 20 mL of acetone was added dropwise to obtain a dark purple solid precipitate, which was then washed once with 5 mL of acetone and separated by high performance liquid chromatography to obtain the vancomycin-porphyrin monomer.

[0035] (2) Preparation of triangular silver nanosheets (AgNPL):

[0036]Add 200μL of 50mM silver nitrate solution and 2mL of 75mM sodium citrate solution into 96mL of ultrapure water in turn, and after rapid stirring,...

Embodiment 2

[0042] Example 2: The composite nano antibacterial material is used for in vitro photodynamic antibacterial treatment of vancomycin-resistant Enterococcus Faecalis (ATCC 51299).

[0043] Pick the monoclonal strain from the LB plate into 5mL LB medium and culture overnight at 37°C. Bacteria were collected by centrifugation at 4000rpm, washed 3 times with sterile PBS buffer, and the OD was adjusted by resuspending the bacterial pellet with PBS buffer 600 is 0.5. Then take 500 μL of bacterial liquid and mix it with different concentrations of composite nano-antibacterial materials and its components, and place it in a 37°C incubator for 15 minutes before performing light or non-light treatment. All illumination experiments were carried out with xenon lamp (400nm-800nm) and the illumination time was 0-2min. Then take 100 μL of the treated bacterial solution and dilute it with sterile PBS buffer to a certain number of times, add it to an LB culture plate, and culture it overnight...

Embodiment 3

[0045] Example 3: The composite nano antibacterial material is used for in vitro photodynamic antibacterial treatment of vancomycin-resistant Enterococcus faecalis (ATCC 51299).

[0046] Pick the monoclonal strain from the LB plate into 5mL LB medium and culture overnight at 37°C. Bacteria were collected by centrifugation at 4000rpm, washed 3 times with sterile PBS buffer, and the OD was adjusted by resuspending the bacterial pellet with PBS buffer 600 is 0.5. Then take 500 μL of bacterial liquid and mix it with different concentrations of composite nano-antibacterial materials and its components, and place it in a 37°C incubator for 15 minutes before performing light or non-light treatment. All illumination experiments were carried out with xenon lamp (400nm-800nm) and the illumination time was 0-2min. Then the treated bacterial solution was diluted 10 with sterile PBS buffer 6 Multiple times, pipette 100 μL of the diluted bacterial solution and add it to the LB culture pl...

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Abstract

The invention discloses a composite nanometer antibacterial material and a preparing method thereof. The composite nanometer antibacterial material is used for solving the problems that conventional target photosensitive drugs are complex to synthesize and poor in biocompatibility. The nanometer antibacterial material is formed by a triangle silver nanometer sheet of a core-shell structure, silicon dioxide particles and vancomycin-porphyrin molecules on the surface. A core of the triangle silver nanometer sheet serves as a host material to form a surface enhanced Raman scattering (SERS) signal, a silicon dioxide shell serves as a rigid platform to couple the vancomycin-porphyrin molecules to form a poly mode. The synthesis cost of the nanometer antibacterial material is low, the preparing method is simple, and the material has good biocompatibility and tissue permeability; in addition, the invention further discloses application to Raman images and photodynamics treatment of vancomycin drug resistant pathogenic bacteria.

Description

Technical field: [0001] The invention relates to the preparation of a composite nano antibacterial material based on triangular silver nanosheets and its application in the treatment of vancomycin-resistant pathogenic bacteria, which belongs to the technical application of triangular silver nanosheets and photodynamic antibacterial treatment field. Background technique: [0002] Vancomycin (Van for short) is a class of glycopeptide antibiotics used to prevent and treat Gram-positive pathogenic bacteria infections. Because vancomycin has strong sterilizing activity and is not easy to make bacteria resistant, it is only allowed to be used when other antibiotics cannot treat bacterial infections, so it is called "the last line of defense of antibiotics". However, with the overuse of vancomycin in clinical practice, reports of "super bacteria" such as Vancomycin-resistant Enterococci (VRE) have appeared in the world. Due to the lack of effective preventive measures and regulat...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K47/52A61K47/54A61K41/00A61K38/14A61P31/04A61K49/00
CPCA61K38/14A61K41/0071A61K49/0013
Inventor 江婷婷王远芳刘佩龙
Owner LUDONG UNIVERSITY
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