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Application of sodium butyrate in preventing and curing high permeability of blood vessels and relevant diseases

A vascular permeability and sodium butyrate technology, applied in the medical field, can solve the problems of related protein expression and function changes, and achieve the effects of repairing mucosal epithelial cells, reducing the pH of the gastrointestinal tract, and promoting proliferation.

Inactive Publication Date: 2016-03-16
ARMY MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, under hypoxic conditions, the expression and function changes of endothelial cell adhesion links and related proteins have not been reported so far.

Method used

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  • Application of sodium butyrate in preventing and curing high permeability of blood vessels and relevant diseases
  • Application of sodium butyrate in preventing and curing high permeability of blood vessels and relevant diseases
  • Application of sodium butyrate in preventing and curing high permeability of blood vessels and relevant diseases

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Effects of sodium butyrate on the permeability of vascular skin cells under hypoxic conditions:

[0020] 1. Materials and reagents: human umbilical vein endothelial cells (HUVEC), cell culture medium DMEM, fetal bovine serum, bovine serum albumin, albumin detection kit

[0021] 2. Test equipment: cell incubator, microplate reader

[0022] 3. Test method: the permeability of monolayer endothelium was detected by transwell chamber. HUVEC to 10×10 4 The density was inoculated on the pre-balanced transwell membrane, and when the entire transwell membrane was covered, the upper chamber was replaced with a medium containing 2% bovine serum albumin (BSA), and corresponding experimental treatments were carried out. At each time point, recover the upper and lower chamber liquids of the transwell chamber, use the QuantichromBcGAlbuminAssayKit kit to detect the BSA concentration in each chamber liquid, and calculate and represent the monolayer endothelial permeability according ...

Embodiment 2

[0026] The effect of hypoxia on the expression of p120 protein in vascular endothelial cells:

[0027] 1. Materials and reagents: human umbilical vein endothelial cells (HUVEC), cell culture medium DMEM, fetal bovine serum, cell protein extract, anti-p120 antibody, anti-β-Catenin antibody, ECL development kit

[0028] 2. Test equipment: gel electrophoresis tank; protein transfer tank; electrophoresis instrument

[0029] 3. Test method:

[0030] Hypoxia (1% O 2 ) condition, the expression level of p120 protein in human umbilical vein endothelial cells (HUVEC). Collect the HUVEC cells treated in each experimental group, lyse the cells with an appropriate amount of cell lysate containing urea, scrape the cells and recover the lysed cells into the corresponding EP tubes, sonicate on ice for 60s, centrifuge at 12000g for 30min, and recover the supernatant as the total cell volume. protein. Protein concentration was determined by BCA method. Take a 20ug protein sample, add 1 / 4 ...

Embodiment 3

[0033] 1. Materials and reagents: human umbilical vein endothelial cells (HUVEC), cell culture medium DMEM, fetal bovine serum, cell protein extract, anti-p120 antibody, ECL development kit

[0034]2. Test equipment: gel electrophoresis tank; protein transfer tank; electrophoresis instrument

[0035] 3. Test method:

[0036] Hypoxia (1% O 2 ) condition, the expression level of p120 protein in human umbilical vein endothelial cells (HUVEC). Collect the HUVEC cells treated by each experimental group, lyse the cells with an appropriate amount of cell lysate containing urea, scrape the cells and recover the lysed cells into the corresponding EP tubes, sonicate on ice for 60s, centrifuge at 12000g for 30min, and recover the supernatant as the total cell volume. protein. Protein concentration was determined by BCA method. Take a 20ug protein sample, add 1 / 4 volume of SDS-PAGE protein sample buffer (5×), boil the protein for 10 min at 100°C, load the sample, and perform SDS polya...

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Abstract

The invention discloses application of sodium butyrate in preparing medicine used for treating and restraining diseases or symptoms relevant to blood vessel permeability enhancement or postponing outbreak of the diseases or symptoms or abating the caused diseases or symptoms. The sodium butyrate can increase the expression of vascular endothelial cell adherent junction gap-associated protein p120 on the oxygen deficit condition, endothelial cell adherent junction stability is kept, and blood vessel endothelium permeability is restrained from being increased on the oxygen deficit condition; the sodium butyrate is soluble in an aqueous solution and stable in property, and not matter dry powder or the aqueous solution can be preserved at normal temperature. The sodium butyrate can be completely and directly absorbed by the intestinal tract, secondary degradation is avoided, and the price is low.

Description

technical field [0001] The invention relates to a medical field, in particular to an application of sodium butyrate in preventing and treating vascular hyperpermeability and related diseases. Background technique [0002] Increased vascular endothelial permeability refers to a series of pathological changes in the morphology of vascular endothelial cells or the destruction of intercellular junction complexes caused by various combined factors, resulting in increased intercellular gaps, impaired barrier function, and rapidly increased permeability. Change. Increased permeability of vascular endothelial cells allows small molecules (ions, water, nutritional molecules), macromolecules, plasma proteins, and cell components to pass through the vascular endothelium abnormally or excessively, which is an important link that causes tissue edema and inflammatory invasion. therefore. How to maintain the integrity of vascular endothelium and inhibit the increase of endothelial permea...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/19A61P7/10A61P9/14A61P25/00A61P11/00
CPCA61K31/19
Inventor 谭小玲向仕权黄小勇杨诚忠陈婷高钰琪黄缄
Owner ARMY MEDICAL UNIV
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