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A strain of Yarrowia lipolytica with high glucose oxidase production

A technology of Yarrowia lipolytica and glucose oxidase, applied in the field of microorganisms

Active Publication Date: 2018-08-07
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the GOD gene has been expressed in Pichia pastoris GS115, Escherichia coli, Trichoderma reesei, etc., but it has not been studied in the unconventional yeast Yarrowia lipolytica, and Yarrowia lipolytica is considered to be Safe, therefore, can be used in food and drug production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Embodiment 1 bacterial strain fermentation produces glucose oxidase

[0028] The Yarrowia lipolytica of the present invention is activated and cultivated on a YPD plate for a certain stage, inoculated into the seed liquid medium YPD, transferred to the fermentation medium PPB with a 10% inoculation amount after 20 hours, and cultivated on a Biotron 3L tank , liquid volume 1L, inoculum volume 10%, cultured at 28°C, stirring speed 600rpm, ventilation volume 2.0vvm, pH control 5.0. When the dissolved oxygen rebounds for the first time and is greater than 60%, add 30g / L glycerol for a short time, the glycerol flow rate is 7.5g / h, and cultivate until the end of fermentation. Its enzyme activity can reach 134U / ml after 96 hours of cultivation.

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Abstract

The invention discloses a strain of Yarrowia lipolytica with high glucose oxidase production, which belongs to the technical field of microorganisms. The Yarrowia lipolytica of the present invention was deposited in the China Center for Type Culture Collection on December 10, 2015, the deposit address is Wuhan University, Wuhan, China, and the deposit number is CCTCC M 2015736. The bacterial strain of the present invention can use cheap sucrose as a carbon source, is suitable for high-density fermentation, and Yarrowia lipolytica is considered safe, and can be used for industrial production of food, SCP and the like. After the bacterial strain of the present invention uses glycerol as the sole carbon source and ferments for 96 hours in a 3L fermenter, the enzyme activity of glucose oxidase reaches 134U / ml.

Description

technical field [0001] The invention relates to a strain of Yarrowia lipolytica with high glucose oxidase production, belonging to the technical field of microorganisms. Background technique [0002] Glucose Oxidase (Glucose Oxidase, referred to as GOD, E.C.1.1.3.4) is a flavin glycoprotein that can specifically catalyze β-D-glucose to generate gluconic acid and hydrogen peroxide under aerobic conditions. GOD is widely distributed in animals, plants and microorganisms. Due to the rapid growth and reproduction of microorganisms and wide sources, they are the main source of GOD production. The main production strains are Aspergillus niger and Penicillium. At present, the application fields of GOD are continuously expanding, and the demand in domestic and foreign markets is increasing sharply. At present, the application fields of GOD are continuously expanding, and the demand in domestic and foreign markets is increasing sharply. At present, the GOD gene has been expressed ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/16C12N9/04C12R1/645
CPCC12N9/0006C12Y101/03004C12N1/145C12R2001/645
Inventor 张娟陈坚刘晓筱堵国成王彬晨
Owner JIANGNAN UNIV
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