A kind of hydrogel that can be used for cell adhesion and preparation method thereof

A hydrogel and cell technology, applied in medical science, prosthesis, etc., can solve the problems of poor biocompatibility, damage to cells and tissues, and weak mechanical properties of hydrogels, and achieve easy control of synthesis conditions and biophase Good capacitance and low synthesis cost

Inactive Publication Date: 2016-11-23
CHINA UNIV OF PETROLEUM (EAST CHINA)
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the hydrogel formed by physical cross-linking generally has weak mechanical properties, and the cross-linked network is easily destroyed by changes in the external environment such as ionic strength, pH value, and temperature, which generally cannot meet the actual application needs of tissue engineering; Gels usually use traditional methods of synthesizing polymers or techniques such as photopolymerization and radiation polymerization to initiate copolymerization or polycondensation reactions to generate covalent bonds to form a covalently crosslinked network.
In the preparation process, it is often necessary to use a large amount of cytotoxic additives such as photoinitiators, crosslinking agents, and organic solvents, resulting in poor biocompatibility of the hydrogel. At the same time, the photoinitiation method without an initiator is not only relatively efficient Low, and local overheating can damage surrounding cells and tissues

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A kind of hydrogel that can be used for cell adhesion and preparation method thereof
  • A kind of hydrogel that can be used for cell adhesion and preparation method thereof
  • A kind of hydrogel that can be used for cell adhesion and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0073] The preparation method of the hydrogel of the present invention specifically includes the following steps:

[0074] The Ac-IIISLGK-NH prepared above 2 It is mixed with L-glutamine in a molar ratio of 1:1 and synthesized under enzymatic reaction conditions. In enzymatic reaction, Ac-IIISLGK-NH 2 The final concentration of the solution is 7.27 mM, the concentration of glutaminase TG is 0.9 U / mL, and the temperature is controlled at 37° C. for 24 hours to obtain a hydrogel.

[0075] Test: Pipette 10μL of hydrogel sample dropwise on the surface of new mica sheet, after static adsorption for 10s, dry the sample with high-purity nitrogen, scan by AFM, take a complete hydrogel and place it on the surface of the sealing film, which will have a carbon film The 400-mesh copper mesh is covered on the gel and adsorbed for 1 min. After removing the copper mesh, use filter paper to absorb the residual liquid around the copper mesh, stain with 2% uranyl acetate for 1 min, and absorb the res...

Embodiment 2

[0077] The preparation method of the hydrogel of the present invention specifically includes the following steps:

[0078] Put 500μLAc-IIISLGK-NH 2 (16mM) and L-Q (16mM) mixed solution left at room temperature for 24 hours, add 25μL TG solution and the same amount of Ca 2+ After mixing the dependent solution thoroughly, place it in a 37°C water bath for 24 hours to obtain a hydrogel.

[0079] The mechanical strength test of the hydrogel: The Hacker rheometer was used to characterize the mechanical properties (viscoelasticity) of the gel formed by the transglutaminase-catalyzed hydrogel. The measurement module used was a cone with a diameter of 35mm and a taper of 2°. Plate and corresponding sample table, each measurement sample volume is 500μL, rheological experiment temperature is 25℃, after 24h of transglutaminase catalyzed sample reaction, stress scanning is performed at a frequency of 1Hz, and the scanning range is 0.01%-100%. Measure the linear viscoelastic zone of the gel, se...

Embodiment 3

[0088] The preparation method of the hydrogel of the present invention specifically includes the following steps:

[0089] The Ac-IIISLGK-NH prepared above 2 It is mixed with L-glutamine in a molar ratio of 1:1 and synthesized under enzymatic reaction conditions. In enzymatic reaction, Ac-IIISLGK-NH 2 The final concentration of the solution was 16 mM, the concentration of TG was 0.9 U / mL, and the temperature was controlled at 38°C for 36 hours to obtain a hydrogel.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses hydrogel applicable to cell adhesion and a preparation method of the hydrogel. The hydrogel is formed by Ac-IIISLGK-NH2 and L-Glutamine (L-Gln) under the action of an enzymatic reaction, wherein the N-terminal of the Ac-IIISLGK-NH2 is acetylated, the C-terminal of the Ac-IIISLGK-NH2 is aminated, the mole ratio of the Ac-IIISLGK-NH2 to the L-Gln is 1:1, reaction temperature is 37-40 DEG C, and reaction time is 24-48 hours. The hydrogel has the advantages that the prepared hydrogel can allow cells to be well adhered to cell surfaces, is low in toxicity and good in biocompatibility, does not cause the non-specific immunity reaction of lymphocyte, is ideal nano tissue engineering material and is significant to human life health.

Description

Technical field [0001] The invention relates to the technical field of preparation of hydrogels, in particular to a hydrogel that can be used for cell adhesion and a preparation method thereof. Background technique [0002] The ideal tissue engineering material not only needs to have a network structure that supports and connects tissues, but also needs to have advantages in regulating tissue generation and cell physiological activities. At present, most scaffold materials mostly use biological macromolecules (chitosan, alginate, collagen) or synthetic polymers (polyethylene glycol) to provide a microenvironment that supports cell growth. However, problems such as chemical residues, disease source transmission and synthesis cost limit its in-depth application. [0003] Transglutaminase in nature is widely present in the human body, advanced animals, plants and microorganisms. It can catalyze the acyl transfer reaction. It can catalyze the relationship between the γ-carboxamide of ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/22A61L27/52
Inventor 陈翠霞徐海王景新张宇白景琨
Owner CHINA UNIV OF PETROLEUM (EAST CHINA)
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap