A kind of hydrogel that can be used for cell adhesion and preparation method thereof

Abstract

The invention discloses hydrogel applicable to cell adhesion and a preparation method of the hydrogel. The hydrogel is formed by Ac-IIISLGK-NH2 and L-Glutamine (L-Gln) under the action of an enzymatic reaction, wherein the N-terminal of the Ac-IIISLGK-NH2 is acetylated, the C-terminal of the Ac-IIISLGK-NH2 is aminated, the mole ratio of the Ac-IIISLGK-NH2 to the L-Gln is 1:1, reaction temperature is 37-40 DEG C, and reaction time is 24-48 hours. The hydrogel has the advantages that the prepared hydrogel can allow cells to be well adhered to cell surfaces, is low in toxicity and good in biocompatibility, does not cause the non-specific immunity reaction of lymphocyte, is ideal nano tissue engineering material and is significant to human life health.

Description

Technical field [0001] The invention relates to the technical field of preparation of hydrogels, in particular to a hydrogel that can be used for cell adhesion and a preparation method thereof. Background technique [0002] The ideal tissue engineering material not only needs to have a network structure that supports and connects tissues, but also needs to have advantages in regulating tissue generation and cell physiological activities. At present, most scaffold materials mostly use biological macromolecules (chitosan, alginate, collagen) or synthetic polymers (polyethylene glycol) to provide a microenvironment that supports cell growth. However, problems such as chemical residues, disease source transmission and synthesis cost limit its in-depth application. [0003] Transglutaminase in nature is widely present in the human body, advanced animals, plants and microorganisms. It can catalyze the acyl transfer reaction. It can catalyze the relationship between the γ-carboxamide of ...

AI Technical Summary

Problems solved by technology

However, the hydrogel formed by physical cross-linking generally has weak mechanical properties, and the cross-linked network is easily destroyed by changes in the external environment such as ionic strength, pH value, and temperature, which generally cannot meet the actual application needs of tissue engineering; Gels usually use traditional methods of synthesizing polymers or techniques such as photopolymerization and radiation polymerization to initiate copolymerization or polycondensation reactions to generate covalent bonds to form a covalently crosslinked network.

In the preparation process, it is often necessary to use a large amount of cytotoxic additives such as photoinitiators, crosslinking agents, and organic solvents, resulting in poor biocompatibility of the hydrogel. At the same time, the photoinitiation method without an initiator is not only relatively efficient Low, and local overheating can damage surrounding cells and tissues

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