Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Bisphenol A fluorescence polarization immunity analysis and detection method based on adopting AMF as fluorescein marker

A fluorescein labeling and fluorescent marker technology, which is used in fluorescence/phosphorescence, analytical materials, biological testing, etc., to achieve the effect of effective reaction, strong fluorescent signal, and rapid reaction.

Inactive Publication Date: 2016-02-24
深圳市绿诗源生物技术有限公司
View PDF5 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The fluorescence polarization immunoassay method has the advantages of high sensitivity, wide application range, no need for sample pretreatment, and rapid analysis results, etc. Related reports on fluorescence polarization immunoassay with fluorescein-based (AMF) as fluorescein label

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Bisphenol A fluorescence polarization immunity analysis and detection method based on adopting AMF as fluorescein marker
  • Bisphenol A fluorescence polarization immunity analysis and detection method based on adopting AMF as fluorescein marker
  • Bisphenol A fluorescence polarization immunity analysis and detection method based on adopting AMF as fluorescein marker

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] 1. Preparation of AMF fluorescein marker

[0031] Weigh 10.9 mg of bisphenolic acid, 10.4 mg of N-hydroxysuccinimide NHS, and 18 mg of dicyclohexylcarbodiimide DCC, dissolve in 1 mL of N-N dimethylformamide DMF, and stir overnight at room temperature in the dark;

[0032] Next, 1 mg of AMF fluorescein was added to the above reaction solution, and stirred at room temperature for 3 hours to prepare a BVA-AMF fluorescent marker.

[0033] 2. Purification of AMF fluorescent markers:

[0034] with CHCl 3 :CH 3 OH:CH 3 COOH=4:1:0.1 was used as developing solution, separated by thin-layer chromatography at room temperature, separated and purified to obtain AMF fluorescent marker, observed under ultraviolet transilluminator, selected orange-yellow band with Rf=0.99, and eluted with 200 μL methanol extraction.

[0035] 3. Determination of the working concentration of AMF fluorescent markers:

[0036] Dilute different concentrations of AMF markers with 0.025M, pH8.0 borate b...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a bisphenol A fluorescence polarization immunity analysis and detection method based on adopting AMF as a fluorescein marker, and aims to provides a detection method with better specificity and sensitivity. According to the method, the fluorescein marker is synthesized through adopting diphenolic acid BVA as the hapten to couple with AMF fluorescein, bisphenol A is adopted as a standard substance, a bisphenol A monoclonal antibody is adopted as an antibody, and the bisphenol A fluorescence polarization immunity analysis and detection method is established; the invention belongs to the technical field of fluorescence polarization immunity detection technology.

Description

technical field [0001] The invention discloses a fluorescence polarization immunoassay detection method, in particular to a bisphenol A fluorescence polarization immunoassay detection method based on AMF as a fluorescein marker; it belongs to the technical field of fluorescence polarization immunoassay. Background technique [0002] Bisphenol A (Bisphenol A, BPA), whose scientific name is 2,2-bis(4-hydroxyphenyl)propane, is one of the important organic chemical raw materials, mainly used in the production of polycarbonate, unsaturated polyester resin, cyclo Oxygen resin polyphenylene ether resin and other polymer materials, adding bisphenol A in an appropriate amount can make plastic products light and durable. Therefore, bisphenol A is widely used in beverage bottles, baby bottles, toys, dental fillings, etc. During production and life, bisphenol A is easily released into the aquatic environment under strong acid, strong alkali or high temperature environment. Studies have...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64G01N33/58G01N33/577
Inventor 赵肃清宁波刘晶
Owner 深圳市绿诗源生物技术有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com