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Method for extracting purified glycoprotein from internal organs of squid

A technology of squid viscera and glycoprotein, which is applied in the field of extracting edible protein compositions, can solve the problems of pollution, waste of protein resources and the environment, and achieve the effects of reasonable process, simple and feasible operation, and pollution reduction

Inactive Publication Date: 2016-02-24
RONGCHENG GUANGRUN SEAFOOD CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to overcome the disadvantages of waste of protein resources and environmental pollution caused by squid viscera being used as feed raw materials or being discarded, the purpose of the present invention is to provide a method for extracting and purifying glycoprotein using squid viscera with reasonable process, feasible operation and high purity of extracted glycoprotein

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] A method for extracting and purifying glycoproteins using squid viscera, which undergoes the following process steps:

[0020] (1) Raw material selection and processing: select fresh squid viscera as raw material, wash and remove dirt and impurities, and set aside;

[0021] (2) Degreasing with acetone: Quantitatively weigh the cleaned squid viscera obtained in step (1) and put them into a distillation flask, add acetone, wherein the ratio of squid viscera to acetone is 1:8 (m / v), and connect to reflux condensation device; heat the distillation flask in a water bath, and control the temperature at 60°C until the acetone solution in the flask becomes clear again, stop heating, filter the supernatant, collect the filter residue, and set aside;

[0022] (3) Crushing: the filter residue obtained in step (2) is mashed to muddy state with a tissue masher, wherein, the masher is set at a rotating speed of 2000rpm, circulates 8 times, grinds for 60 seconds each time and stops gr...

Embodiment 2

[0029] A method for extracting and purifying glycoproteins using squid viscera, which undergoes the following process steps:

[0030] (1) Raw material selection and processing: choose frozen and thawed fresh squid viscera as raw material, clean and remove dirt and impurities, and set aside;

[0031] (2) Degreasing with acetone: Quantitatively weigh the cleaned squid viscera obtained in step (1) and put them into a distillation flask, add acetone, wherein the ratio of squid viscera to acetone is 1:10 (m / v), and connect to reflux condensation device; heat the distillation flask in a water bath, and control the temperature at 50°C until the acetone solution in the flask becomes clear again, stop heating, filter the supernatant, collect the filter residue, and set aside;

[0032] (3) crushing: the filter residue obtained in step (2) is crushed to muddy state with a tissue masher, subject to the absence of flocculent or massive tissues;

[0033] (4) Leaching: Add sodium chloride s...

Embodiment 3

[0039] A method for extracting and purifying glycoproteins using squid viscera, which undergoes the following process steps:

[0040] (1) Raw material selection and processing: select fresh squid viscera as raw material, wash and remove dirt and impurities, and set aside;

[0041] (2) Degreasing with acetone: Quantitatively weigh the cleaned squid viscera obtained in step (1) and put them into a distillation flask, add acetone, wherein the ratio of squid viscera to acetone is 1:5 (m / v), and connect to reflux condensation device; heat the distillation flask in a water bath, and control the temperature at 65°C until the acetone solution in the flask becomes clear again, stop heating, filter the supernatant, collect the filter residue, and set aside;

[0042] (3) crushing: the filter residue obtained in step (2) is crushed to a muddy state with a tissue masher, and set aside;

[0043] (4) Leaching: Add sodium chloride solution to the squid viscera obtained in step (3) to extract...

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PUM

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Abstract

The invention discloses a method for extracting purified glycoprotein from internal organs of a squid. The method mainly overcomes the problems of waste of protein resources and environmental pollution due to usage of the internal organs of the squid as a feed raw material or discarding of the internal organs of the squid in the prior art. The method comprises the following steps: with fresh or thawed frozen squid internal organs as a raw material, successively carrying out degreasing through acetone hot bath, crushing and then extraction with a sodium chloride solution so as to obtain crude glycoprotein extract; subjecting the crude glycoprotein extract to dialysis, desalination and fractional precipitation via ammonium sulfate so as to obtain a crude glycoprotein product; and subjecting the crude glycoprotein product to Sephadex G-100 gel column chromatography, then carrying out double distilled water equilibrium and elution, separating and purifying crude glycoprotein of the internal organs of the squid and subjecting eluate to freeze drying so as to obtain a purified dry squid internal organ glycoprotein product. The method provided by the invention realizes value-added utilization of the internal organs of the squid, reduces pollution of squid waste to the environment, and is reasonable in process and simple and feasible in operation; and the extracted glycoprotein has high purity and can be produced in large scale.

Description

technical field [0001] The invention relates to extracting edible protein compositions from other marine animals, in particular to a method for extracting and purifying glycoproteins from squid viscera. Background technique [0002] my country's squid processing output is large, in the process of being processed into dried squid, squid flowers, salted squid, canned food and condiments, generally 15-20% of wastes such as viscera, skin, eyes and eye sockets are produced. These wastes are rich in protein and other nutrients, are easy to rot and deteriorate, and contain some heavy metals. The traditional treatment method is to use them as feed materials or discard them, which causes a great waste of protein resources and environmental pollution. [0003] At present, the scope of research on the comprehensive utilization of squid viscera is relatively narrow, mainly because of the fish oil and protein contained in it. Extraction research. "China Oils and Fats" 2011 Volume 36 No....

Claims

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Application Information

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IPC IPC(8): C07K1/36C07K1/34C07K1/30C07K1/16
Inventor 杨劲峰吴文惠张静怡周喻
Owner RONGCHENG GUANGRUN SEAFOOD CO LTD
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