Method for in vitro culture of hemerocallis middendorfii Trautv. et Mey. tissue

A technology for in vitro cultivation of Hemerocallis grandiflorum, applied in horticultural methods, botany equipment and methods, horticulture, etc., can solve the problems of large market demand, small number of introduced species, slow division, etc. The uniformity, cost reduction, and the effect of maintaining the traits of the female parent

Inactive Publication Date: 2016-02-24
上海上房园艺有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

As a variety imported from abroad, the number of introductions is small, the ramets are slow, the market demand is large, and the supply of seedlings is limited

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] A method for in vitro tissue culture of Hemerocallis grandiflora, comprising the following steps:

[0026] (1) The preparation of the culture medium in each culture stage, including the components and contents of the culture medium:

[0027] Bud induction medium: MS+KT0.3mg / L+6-BA2.0mg / L+NAA1mg / L;

[0028] Adventitious bud proliferation medium: MS+6-BA2.0mg / L+NAA0.1mg / L;

[0029] Strong seedling medium: MS+6-BA0.5mg / L+NAA0.05mg / L;

[0030] Rooting medium: MS+NAA0.05mg / ;

[0031] Each of the above media also includes the following components: 30 g / L sucrose, 6 g / L agar; the pH value in the media is 5.8.

[0032] (1) Obtaining sterile materials: Pick the small buds on the flower stems of Hemerocallis grandiflora during the flowering season, wash them with tap water for 1 hour, place them on a clean bench, soak them in 75wt% ethanol for 30s, and use 1wt‰ Soak in mercuric chloride for 15 minutes, rinse with sterile water for 5-6 times, blot the surface with sterile filt...

Embodiment 2

[0039] A method for in vitro tissue culture of Hemerocallis grandiflora, comprising the following steps:

[0040] (1) The preparation of the culture medium in each culture stage, including the components and contents of the culture medium:

[0041] Bud induction medium: MS+KT0.5mg / L+6-BA3.0mg / L+NAA2mg / L;

[0042] Adventitious bud proliferation medium: MS+6-BA3.0mg / L+NAA0.3mg / L;

[0043] Strong seedling medium: MS+6-BA2.0mg / L+NAA0.2mg / L;

[0044] Rooting medium: MS+NAA0.2mg / ;

[0045] Each of the above media also includes the following components: 30 g / L sucrose, 6 g / L agar; the pH value in the media is 5.8.

[0046] (1) Obtaining sterile materials: Pick the small buds on the flower stems of Hemerocallis grandiflora during the flowering season, wash them with tap water for 1 hour, place them on a clean bench, soak them in 75wt% ethanol for 30s, and use 1wt‰ Soak in mercuric chloride for 15 minutes, rinse with sterile water for 5-6 times, blot the surface with sterile filter...

Embodiment 3

[0053] A method for in vitro tissue culture of Hemerocallis grandiflora, comprising the following steps:

[0054] (1) The preparation of the culture medium in each culture stage, including the components and contents of the culture medium:

[0055] Bud induction medium: MS+KT0.4mg / L+6-BA2.5mg / L+NAA1.5mg / L;

[0056] Adventitious bud proliferation medium: MS+6-BA2.5mg / L+NAA0.2mg / L;

[0057] Strong seedling medium: MS+6-BA1mg / L+NAA0.1mg / L;

[0058] Rooting medium: MS+NAA0.1mg / ;

[0059] Each of the above media also includes the following components: 30 g / L sucrose, 6 g / L agar; the pH value in the media is 5.8.

[0060] (1) Obtaining sterile materials: Pick the small buds on the flower stems of Hemerocallis grandiflora during the flowering season, wash them with tap water for 1 hour, place them on a clean bench, soak them in 75wt% ethanol for 30s, and use 1wt‰ Soak in mercuric chloride for 15 minutes, rinse with sterile water for 5-6 times, blot the surface with sterile filter...

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Abstract

The invention relates to a method for in vitro culture of hemerocallis middendorfii Trautv. et Mey. tissue. The method comprises the following steps: picking a small bud on a flower stalk when hemerocallis middendorfii Trautv. et Mey. is in the blooming season, performing sterile processing on the small bud, and sequentially performing bud differentiation and proliferation, strong adventitious bud seedling culture, rooting culture, seedling exercising and transplanting to obtain the hemerocallis middendorfii Trautv. et Mey. Compared with the prior art, the year propagation coefficient of the hemerocallis middendorfii Trautv. et Mey. can be increased to 1: 100,000 or even higher, and the cost can be reduced to 0.5-0.8 yuan / strain, so that the reproduction rate and the uniformity of seedlings are greatly improved, and original female parent characters are better maintained.

Description

technical field [0001] The invention relates to a tissue in vitro culture method, in particular to a tissue in vitro culture method of Hemerocallis grandiflorum. Background technique [0002] Hemerocallis grandiflora is a perennial evergreen herb of the genus Hemerocallis in the family Liliaceae. The leaves are tender green. It blooms from May to June. The flowers are large, funnel-shaped, about 10cm in diameter, rose red, semi-double, bright in color, easy to cultivate, and evergreen, with beautiful green leaves in clusters. In the garden, it is often planted in clusters or planted in flower borders and roadsides. Hemerocallis grandiflora is tolerant to half shade and can be used as a ground cover in sparse forests. It is a good flower mirror and garden material. As the species introduced from abroad, the number of introductions is small, the ramets are slow, the market demand is large, and the supply of seedlings is limited. [0003] Chinese patent 201010190810.X discl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 陈建华黄建荣李霖
Owner 上海上房园艺有限公司
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