Rapid acanthus spinosus propagation method based on tissue culture

A technology of tissue culture and rat bougainvillea, applied in the field of plant breeding, can solve the problems of large differences in breeding environments, achieve the effects of increasing the speed of reproduction, improving the uniformity of seedlings, and reducing costs

Inactive Publication Date: 2018-04-03
上海上房园艺有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the large differences in the habits and the required breeding environment between New Zealand hemp and spiny rat bougainvillea, the above tissue culture method cannot be directly used in the breeding process of spiny rat bouquet

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] A method for rapid breeding of spiny mouse bougainvillea based on tissue culture, comprising the following steps:

[0033] (1) The preparation of the culture medium, including the components and contents of the culture medium at each stage of tissue culture:

[0034] 1.1. Bud induction medium: MS+BA 3mg / L+NAA 0.3mg / L;

[0035] 1.2. Adventitious bud proliferation medium: MS+6-BA 1mg / L+NAA 0.1mg / L;

[0036] 1.3. Strong seedling medium: MS+6-BA 0.5mg / L+NAA 0.1mg / L;

[0037] 1.4. Rooting medium: MS+NAA 0.2mg / L;

[0038] The pH of the above various media is 5.8, add 30g / L sucrose, 6g / L agar, culture temperature is 25°C, light is 1800Lx;

[0039] (2) Tissue culture of spiny mouse bougainvillea:

[0040] 2.1. Obtaining sterile materials

[0041] The small shoots of the spiny mouse bougainvillea were dug from the ground in March-April in spring, rinsed with tap water for 1 hour, and then placed on an ultra-clean workbench, soaked in 75% ethanol for 30 seconds, soaked in 1‰...

Embodiment 2

[0051] A method for rapid breeding of spiny mouse bougainvillea based on tissue culture, comprising the following steps:

[0052] (1) The preparation of the culture medium, including the components and contents of the culture medium at each stage of tissue culture:

[0053] 1.1. Bud induction medium: MS+BA 1mg / L+NAA 0.1mg / L;

[0054] 1.2. Adventitious bud proliferation medium: MS+6-BA 0.5mg / L+NAA 0.05mg / L;

[0055] 1.3. Strong seedling medium: MS+6-BA 0.1mg / L+NAA 0.05mg / L;

[0056] 1.4. Rooting medium: MS+NAA 0.1mg / L;

[0057] The pH of the above various media is 5.8, add 30g / L sucrose, 6g / L agar, culture temperature is 24°C, light is 1700Lx;

[0058] (2) Tissue culture of spiny mouse bougainvillea:

[0059] 2.1. Obtaining sterile materials

[0060] The small shoots of the spiny mouse bougainvillea were dug from the ground in March-April in spring, rinsed with tap water for 1 hour, and then placed on an ultra-clean workbench, soaked in 75% ethanol for 30 seconds, soaked i...

Embodiment 3

[0070] A method for rapid breeding of spiny mouse bougainvillea based on tissue culture, comprising the following steps:

[0071] (1) The preparation of the culture medium, including the components and contents of the culture medium at each stage of tissue culture:

[0072] 1.1. Bud induction medium: MS+BA 3mg / L+NAA 0.3mg / L;

[0073] 1.2. Adventitious bud proliferation medium: MS+6-BA 1.5mg / L+NAA 0.15mg / L;

[0074] 1.3. Strong seedling medium: MS+6-BA 1mg / L+NAA 0.15mg / L;

[0075] 1.4. Rooting medium: MS+NAA 0.3mg / L;

[0076] The pH of the above-mentioned various media is 5.8, 30g / L sucrose, 6g / L agar are added, the culture temperature is 26°C, and the light is 1900Lx;

[0077] (2) Tissue culture of spiny mouse bougainvillea:

[0078] 2.1. Obtaining sterile materials

[0079] The small shoots of the spiny mouse bougainvillea were dug from the ground in March-April in spring, rinsed with tap water for 1 hour, and then placed on an ultra-clean workbench, soaked in 75% ethano...

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PUM

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Abstract

The invention relates to a rapid acanthus spinosus propagation method based on tissue culture. The method comprises the steps as follows: (1) preparation of a bud induction medium, an adventitious budproliferation medium, a seedling strengthening medium and a rooting medium; (2) tissue culture of acanthus spinosus. Compared with the prior art, the method has the advantages that by means of the tissue culture technology, the annual propagation coefficient is increased to 1:40,000, the cost is decreased by 0.6-0.8 yuan / plant, the propagation speed and uniformity of acanthus spinosus tissue cultured seedlings are greatly increased, and original female parent characters are better kept.

Description

technical field [0001] The invention relates to a plant breeding method, in particular to a tissue culture-based rapid breeding method of spiny rat bougainvillea. Background technique [0002] Prickly mouse bougainvillea is a plant of the genus Acanthaceae, a perennial herb, with a plant height of 70-100 cm, leaves broadly oval or oblong, and 1-2 times pinnately deeply lobed. Spikes, small flowers are dense, purple, flowering period is June-July. Because of its unique flower shape and strong shade tolerance, it can be planted in a large area, and it is an excellent material for ground cover plants in forests. As an excellent garden plant, it is gradually recognized by everyone because of its ornamental, stress resistance and novelty. Prickly rat bougainvillea can be propagated by sowing and division, but the seeds are less fruitful, and the division is slow, the market demand is large, and the supply of seedlings is limited. [0003] Chinese patent CN103734019A discloses ...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/008A01H4/001
Inventor 陈建华黄建荣金梦怡李霖庞玉华
Owner 上海上房园艺有限公司
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