Application of raltitrexed or pharmaceutically acceptable salt thereof in preparation of medicine for treating or preventing herpes virus infection

A technology of herpes virus infection and raltitrexed, which is applied in the field of biomedicine to achieve the effect of good anti-herpes virus activity

Inactive Publication Date: 2018-01-09
WUHAN INST OF VIROLOGY CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, drugs to treat or prevent herpes virus infection still need to be further developed

Method used

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  • Application of raltitrexed or pharmaceutically acceptable salt thereof in preparation of medicine for treating or preventing herpes virus infection
  • Application of raltitrexed or pharmaceutically acceptable salt thereof in preparation of medicine for treating or preventing herpes virus infection
  • Application of raltitrexed or pharmaceutically acceptable salt thereof in preparation of medicine for treating or preventing herpes virus infection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Detection of the activity of raltitrexed in inhibiting KSHV lytic phase replication

[0034] 1. Experimental materials

[0035] 1.1 Cells and viruses

[0036]iSLK.219 cells were donated by Professor Dr. Don Ganem. The cells are SLK cells containing recombinant virus rKSHV.219 and doxycycline (Doxycycline) regulated RTA expression system. In this cell, the RTA expression sequence was constructed into the pRetro-X Tet-ON inducible expression system (Clontech, Mountainview, CA), and the expression of this system was regulated by doxycycline. In the presence of doxycycline, RTA expression can occur; Vero cells were preserved in our laboratory and purchased from American Type Culture Collection (ATCC).

[0037] Raltitrexed was purchased from Biochempartner.

[0038] 1.2 Reagents

[0039] DMEM medium, 1640 medium and FBS were purchased from GIBCO; Alamarblue activity detection kit was purchased from Promega; SYBR mixture (iTaq TM Universal Green Supermix) was purchased...

Embodiment 2

[0065] Embodiment 2: the detection of raltitrexed anti-HSV-1 and HSV-2 activity

[0066] 1. Experimental materials

[0067] 1.1 Cells and viruses

[0068] Vero cells were preserved in our laboratory and purchased from the American Type Culture Collection (ATCC). HSV-1 is an F strain, which is preserved by the Wuhan Institute of Virology, Chinese Academy of Sciences. HSV-2 is a G strain, which is preserved by the Wuhan Institute of Virology, Chinese Academy of Sciences.

[0069] 1.2 Reagents

[0070] DMEM medium and FBS were purchased from GIBCO; Alamarblue activity detection kit was purchased from Promega.

[0071] 1.3 Experimental Instruments

[0072] The multi-label microplate reader and the high-content cell analyzer were purchased from PerkinElmer; the cell incubator was a product of Thermo.

[0073] 2. Experimental methods and results

[0074] 2.1 Cytotoxicity detection of raltitrexed on Vero cells

[0075] (1) Divide Vero cells into 8×10 3 Each cell / well was see...

Embodiment 3

[0094] Embodiment 3: Raltitrexed inhibits the detection of EBV lytic phase replication

[0095] 1. Experimental materials

[0096] 1.1 Cells and viruses

[0097] The B95-8 cell was donated by Professor Sun Xiaoping of the School of Basic Medicine of Wuhan University. The cell contains the complete sequence of the EBV virus genome. Combined induction with 20ng / mL TPA can make the virus enter the lytic phase and replicate.

[0098] 1.2 Reagents

[0099] 1640 medium and FBS were purchased from GIBCO; Alamarblue activity detection kit was purchased from Promega; SYBR mixture (iTaq TM Universal Green Supermix) was purchased from Bio-Rad Company.

[0100] 1.3 Experimental Instruments

[0101] The multi-marker microplate reader was purchased from PerkinElmer; the fluorescent quantitative PCR instrument (Bio-RadCFX96Touch TM Real-Time PCR detection system) was purchased from Bio-Rad Company; Type refrigerated centrifuge and cell culture box were purchased from Thermo Company...

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Abstract

The invention discloses the application of raltitrexed or a pharmaceutically acceptable salt thereof in the preparation of a drug for treating or preventing herpes virus infection. The invention selects raltitrexed with a completely non-toxic concentration to conduct an anti-herpes virus experiment, and the results show that The compound has significant anti-herpes virus activity in a dose-dependent manner, and raltitrexed shows its anti-herpes virus effect by inhibiting virus replication. The detection of different subtypes of herpes virus shows that raltitrexed has broad-spectrum anti-herpes virus activity and high selection index, and can be developed as a drug for treating herpes virus infection diseases, and has broad application prospects.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular, the invention relates to the application of raltitrexed or a pharmaceutically acceptable salt thereof in the preparation of medicines for treating or preventing herpes virus infection. Background technique [0002] Herpesviridae is a family of viruses with envelopes and double-stranded DNA genomes. Members of this family can widely infect animals and humans and induce corresponding diseases. There are currently eight herpes viruses that can infect humans, mainly including: Herpes simplex virus-1 (HSV-1), Herpes simplex virus-2 (HSV-2), Varicella zoster virus (VZV), Epstein-Barr virus (EBV), cytomegalovirus (CMV), human herpes virus type VI (HHV-6), human herpes virus type VII (HHV-7) and Kaposi's sarcoma-associated herpesvirus (KSHV). According to the differences in genome sequence, structure and physical and chemical properties, Herpesviridae can be divided into three subfamilies: α-...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/4709A61P31/22
Inventor 陈绪林陈军刚
Owner WUHAN INST OF VIROLOGY CHINESE ACADEMY OF SCI
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