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LMNB2 gene and expression product thereof as diagnosing and treating target for nasopharyngeal carcinoma

A technology for gene expression and nasopharyngeal carcinoma, which can be used in gene therapy, microbial detection/testing, disease diagnosis, etc., and can solve the problems of lack of sensitivity and specificity

Active Publication Date: 2016-02-03
QINGDAO MEDINTELL BIOMEDICAL CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although these indicators alone or in combination provide useful information for the diagnosis of NPC, they lack sufficient sensitivity and specificity

Method used

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  • LMNB2 gene and expression product thereof as diagnosing and treating target for nasopharyngeal carcinoma
  • LMNB2 gene and expression product thereof as diagnosing and treating target for nasopharyngeal carcinoma
  • LMNB2 gene and expression product thereof as diagnosing and treating target for nasopharyngeal carcinoma

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] Differential expression of embodiment 1 LMNB2 gene

[0063] 1. Research object:

[0064] 40 cases of patients diagnosed with nasopharyngeal carcinoma and 40 cases of patients diagnosed with chronic inflammation of nasopharyngeal carcinoma (serving as normal nasopharyngeal epithelial tissue control), respectively obtained nasopharyngeal carcinoma tissue and normal nasopharyngeal epithelial tissue by microdissection technology .

[0065] 2. Acquisition of RNA from nasopharyngeal carcinoma tissue and normal nasopharyngeal epithelial tissue

[0066] Total RNA was extracted from nasopharyngeal carcinoma tissue and normal nasopharyngeal epithelial tissue using Trizol one-step method, and the purity of the RNA solution was determined by reading the absorbance values ​​at 260nm and 280nm by NanodropND-1000 (A). The integrity of RNA was detected by electrophoresis on 1% formaldehyde denatured agarose gel and observed under ultraviolet transmission light.

[0067] 3. Gene chip...

Embodiment 2

[0075] Example 2 Differential expression of LMNB2 protein

[0076] 1. The research object is the same as in Example 1.

[0077] 2. Extract tissue total protein

[0078] Follow the instructions of the EpiQuik Tissue / Cell Total Protein Extraction Kit for protein extraction.

[0079] 3. Western blot detection

[0080] The extracted protein was quantitatively subjected to SDS-PAGE electrophoresis, followed by membrane transfer, blocking, primary antibody incubation, secondary antibody incubation, and color development.

[0081] 4. Statistical processing

[0082] The gray value of the protein band was analyzed using ImageJ software, and β-actin was used as an internal reference to normalize the gray value of the LMNB2 protein band. The results and data are expressed in the form of mean ± standard deviation, and SPSS13.0 statistical software is used for statistical analysis. The difference between the two is tested by t test, and it is considered statistically significant when P...

Embodiment 3

[0085] Embodiment 3 suppresses LMNB2 gene expression

[0086] 1. siRNA design and synthesis

[0087] siRNA sequences targeting LMNB2:

[0088] siRNA1-LMNB2:

[0089] The sense strand is 5'-UGUAAACGGUGAGCUAUUGCC-3' (SEQ ID NO.7);

[0090] The antisense strand is 5'-CAAUAGCUCACCGUUUACACC-3' (SEQ ID NO.8),

[0091] siRNA2-LMNB2:

[0092] The sense strand is 5'-AAUUCUCUAGAAAUGUAUCAA-3' (SEQ ID NO.9);

[0093] The antisense strand is 5'-GAUACAUUUCUAGAGAAUUUC-3' (SEQ ID NO.10),

[0094] siRNA3-LMNB2:

[0095] The sense strand is 5'-UUAGUGUUUCCAAAGACUCAA-3' (SEQ ID NO.11);

[0096] The antisense strand is 5'-GAGUCUUUGGAAACACUAAGA-3' (SEQ ID NO.12)

[0097] Negative control siRNA sequence (siRNA-NC):

[0098] The sense strand is 5'-CGUACGCGGAAUACUUCGA-3' (SEQ ID NO.13);

[0099] The antisense strand is 5'-UCGAAGUAUUCCGCGUACG-3' (SEQ ID NO.14).

[0100] 2. Culture and transfection of nasopharyngeal carcinoma cells

[0101] The human nasopharyngeal carcinoma cell line HONE-1...

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Abstract

The invention discloses a LMNB2 gene and an expression product thereof as a diagnosing and treating target for nasopharyngeal carcinoma. If a tested person is suffered from nasopharyngeal carcinoma or has the risk in suffering from nasopharyngeal carcinoma can be judged by detecting the content of LMNB2 gene and the expression product thereof in the bone tissue. According to the invention, the research on the multiplication condition of the nasopharyngeal carcinoma cells cultured in vitro proves that the expression of the LMNB2 gene can restrain the multiplication of the nasopharyngeal carcinoma cells, so that the multiplication of the nasopharyngeal carcinoma cells can be restrained by restraining the function of the LMNB2 protein. The result of the research proves that the LMNB2 gene and the expression product thereof are served as the potential drug targets for treating the nasopharyngeal carcinoma.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to the use of LMNB2 gene in the diagnosis and treatment of nasopharyngeal carcinoma. Background technique [0002] Nasopharyngeal carcinoma is a malignant tumor that originates from the lining epithelium of the nasopharyngeal mucosa. It is one of the common malignant tumors in my country, with high frequency of malignancy, and the average natural survival time is 18.7 months. China's Guangdong, Guangxi, Fujian, Hunan and other places are high-incidence areas, with more men than women. The age of onset is mostly middle-aged, and there are also adolescent patients. The etiology is related to racial susceptibility (yellow race is more affected than white race), genetic factors, and Epstein-Barr virus infection. Nasopharyngeal carcinoma has a high degree of malignancy, and cervical lymph node metastasis can occur in the early stage. [0003] The location of nasopharyngeal carcinoma is hi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68G01N33/68A61K45/00A61K48/00A61K31/7088A61K39/395A61P35/00
CPCA61K31/7088A61K39/395A61K45/00C12Q1/6886C12Q2600/158G01N33/57484G01N33/6854G01N2800/14
Inventor 杨承刚孙锦云
Owner QINGDAO MEDINTELL BIOMEDICAL CO LTD
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