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A biofilm for guided periodontal tissue regeneration and its preparation method and application

A periodontal tissue regeneration and biofilm technology, applied in medical science, prosthesis, etc., can solve the problems of insufficient oxygen supply in the early stage, and achieve the effects of improving survival rate, inhibiting breeding, and improving efficiency

Inactive Publication Date: 2019-05-14
FOURTH MILITARY MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to provide a biofilm for guiding periodontal tissue regeneration and its preparation method and application. The biofilm can improve the efficiency of periodontal tissue regeneration and solve the early problems that occur when the existing biofilm guides periodontal tissue regeneration. Insufficient oxygen supply

Method used

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  • A biofilm for guided periodontal tissue regeneration and its preparation method and application
  • A biofilm for guided periodontal tissue regeneration and its preparation method and application

Examples

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Effect test

example 1

[0046] Egg yolk lecithin was weighed and added to Tyrode's salt buffer to prepare a mixed solution of egg yolk lecithin and Tyrode's salt buffer with a mass volume percentage concentration of 15%. Under 280W power, ultrasonic emulsification was performed 3 times for 10 seconds each time. Interval 1 minute between every two ultrasonic emulsifications, prepare base emulsion; Get perfluorotriethylamine stock solution (concentration: 98%) in addition, base emulsion and perfluorotriethylamine stock solution are mixed by volume ratio 8:1, and Under the power of 280W, ultrasonic emulsification was carried out 8 times, each time for 10 seconds, and an interval of 1 minute between each two ultrasonic emulsifications was used to prepare a water-soluble perfluorotriethylamine emulsion; the prepared perfluorotriethylamine emulsion was placed in into the hyperbaric oxygen chamber for 8 minutes to make the perfluorotriethylamine emulsion fully dissolve oxygen and make it reach a saturated st...

example 2

[0048] Egg yolk lecithin was weighed and added to Tyrode's salt buffer to prepare a mixed solution of egg yolk lecithin and Tyrode's salt buffer with a mass volume percentage concentration of 16%. Under 300W power, ultrasonic emulsification was performed 3 times for 20 seconds each time. The base emulsion was prepared at intervals of 1 minute between every two ultrasonic emulsifications; another perfluorotriethylamine stock solution (concentration greater than 98%) was taken, the base emulsion and the perfluorotriethylamine stock solution were mixed at a volume ratio of 4:1, and Under the power of 300W, ultrasonic emulsification was carried out 10 times, each time for 20 seconds, with an interval of 1 minute between each two ultrasonic emulsifications, and a water-soluble perfluorotriethylamine emulsion was prepared; the prepared perfluorotriethylamine emulsion was placed in into the hyperbaric oxygen chamber for 10 minutes to make the perfluorotriethylamine emulsion fully diss...

example 3

[0050] Egg yolk lecithin was weighed and added to Tyrode's salt buffer to prepare a mixed solution of egg yolk lecithin and Tyrode's salt buffer with a mass volume percentage concentration of 16.6%. Under 320W power, ultrasonic emulsification was performed 3 times for 20 seconds each time. The base emulsion was prepared at an interval of 1 minute between every two ultrasonic emulsifications; another perfluorotriethylamine stock solution (concentration greater than 98%) was taken, and the base emulsion and the perfluorotriethylamine stock solution were mixed in a volume ratio of 3:2, and Under the power of 320W, ultrasonic emulsification was performed 12 times, each time for 20 seconds, and the interval between each two ultrasonic emulsification was 1 minute, and a water-soluble perfluorotriethylamine emulsion was prepared; the prepared perfluorotriethylamine emulsion was placed in the into the hyperbaric oxygen chamber for 12 minutes to make the perfluorotriethylamine emulsion ...

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Abstract

The invention discloses a biomembrane for guided tissue regeneration of periodontium, and a preparation method and application thereof. The method comprises the following steps: culturing periodontal ligament stem cells and preparing aseptic oxygen-rich perfluorotriethylamine emulsion; mixing the periodontal ligament stem cells and the perfluorotriethylamine emulsion to prepare cell-perfluorotriethylamine emulsion which includes 104 to 108 cells per ml of the perfluorotriethylamine emulsion; and inoculating the prepared cell-perfluorotriethylamine emulsion on a biomembrane for culture, wherein the biomembrane for guided tissue regeneration of periodontium is obtained after termination of the culture. The preparation method for the biomembrane inhibits propagation of local anacerobes while improving the early-stage survival rate of the periodontal ligament stem cells after implantation by improving a hypoxia environment in the biomembrane, so the failure rate of guided tissue regeneration is greatly reduced, the success rate of guided tissue regeneration is improved, and good economic and social benefits are obtained.

Description

【Technical field】 [0001] The invention belongs to the technical field of biofilm preparation, in particular to a biofilm for guided periodontal tissue regeneration and its preparation method and application. 【Background technique】 [0002] Periodontitis is the leading cause of tooth loss in humans. Periodontal tissue regeneration is the ultimate goal of periodontal disease treatment, but traditional basic treatments such as periodontal scaling and scaling can only eliminate the cause, but cannot repair the lost periodontal tissue. The common method of guided tissue regeneration in periodontal treatment is to use membrane material as a barrier in periodontal surgery to prevent the gingival epithelium from growing along the root surface during the healing process, prevent the gingival connective tissue from contacting the root surface, and provide a certain space, guiding periodontal ligament cells with the ability to form new attachments to preferentially occupy the root sur...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/38A61L27/52A61L27/24
Inventor 鹿蕾张旭黄景辉
Owner FOURTH MILITARY MEDICAL UNIVERSITY
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