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Application of protein tyrosine kinase fyn oncogene in prevention and treatment of enterovirus 71 infection

A technology of tyrosine kinase and enterovirus, which is applied to the new target and application field of anti-enterovirus 71 infection

Active Publication Date: 2018-11-30
SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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Problems solved by technology

[0008] At present, there is no research report on the role of FYN and EFS molecules in EV71 infection of HBMEC. In-depth research on FYN and EFS molecules can not only improve the understanding of EV71 infection and pathogenic mechanism, but also provide new information for the prevention and treatment of EV71 infection. ideas and goals

Method used

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  • Application of protein tyrosine kinase fyn oncogene in prevention and treatment of enterovirus 71 infection
  • Application of protein tyrosine kinase fyn oncogene in prevention and treatment of enterovirus 71 infection
  • Application of protein tyrosine kinase fyn oncogene in prevention and treatment of enterovirus 71 infection

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Embodiment 1

[0057] 1 Design and synthesis of specific siRNA sequences for each host cell molecule.

[0058] 1.1 For each target gene, search NCBI GeneBank to obtain the full sequence and mRNA sequence, use existing network resources and commonly used software to conduct biological analysis of each target gene, and select the coding region as the target sequence for siRNA design. Refer to the siRNA design principle, and compare it with the human genome sequence through the blast function of the GeneBank database to ensure that there is no homology; exclude potential siRNAs that have 8 consecutive bases at the 5' end of the aitisense chain paired with other genes; exclude any consecutive 14 bases base-pairing potential siRNAs with other genes. The design software was used for pre-evaluation and determination, and three targets with the best kinetic parameters were selected to enter the subsequent experimental process. A total of three interference sequences were synthesized for each gene, a...

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Abstract

The invention relates to the technical field of biomedicine, in particular to a novel target resistant to enterovirus 71 infections and application thereof. Brain microvascular endothelial cells (HBMEC) are used as target cells, the expression of host proteins of the target cells are down-regulated by using the RNA interference technology, and thus host factors effective in inhibiting EV71 infections for brain microvascular endothelial cells (HBMEC) are thus found, the blood brain barrier function is protected, and the virus is prevented from penetrating the blood brain barrier to infect the central nervous system. Experiments show that protein tyrosine kinase FYN proto-oncogene (FYN proto-oncogene, Src family tyrosine kinase) molecules play an important role in the HBMEC infection by EV71, and EV71 infections can be significantly inhibited by down-regulating the expression of FYN. The invention provides application of the FYN in the preparation of drugs for preventing or treating enterovirus 71 infections.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to a new target and application of anti-enterovirus 71 infection. Background technique [0002] Enterovirus 71 (EV71) belongs to the Picornaviridae Enterovirus genus Human Enterovirus A, and is one of the main pathogens that cause Hand-foot-mouth disease (HFMD). Currently, hand, foot and mouth disease is breaking out and spreading in many parts of the world, especially in the Asia-Pacific region. In my country, since the outbreak of hand, foot and mouth disease in several major provinces and cities in 2008, the number of people infected and the death rate of the disease have remained high, with more than 1 million cases and nearly 1,000 deaths reported every year. The main patients with HFMD are infants and young children under 5 years old. The clinical manifestations are fever, herpes and other symptoms on the hands, feet, buttocks and oral mucosa; a small number of children c...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K48/00A61K31/7088A61P31/14C12Q1/6886
Inventor 朱勇喆戚中田徐庆强赵平陈生林
Owner SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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