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Method for ex-situ preparation of methane and combined production of humic acid, and composite microbial agent used in same

A compound bacterial agent and in-situ preparation technology is applied in the field of lignite resource utilization to achieve the effect of improving methane production, good effect and gas production.

Inactive Publication Date: 2016-01-27
创盛量子科技(温州)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above-mentioned patents all use physical and chemical methods to process lignite to produce humic acid, which involves acid-base and high-temperature treatment. While obtaining the product, it also creates a certain pressure on the environment. Microbiological methods are rarely used to process lignite to produce methane and humic acid at the same time.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] The preparation of embodiment 1 fermented liquid

[0028] This embodiment only takes Escherichia coli (ATCC11775), Pseudomonas putida (CICC21906), Penicillium decumbens (CGMCC3.7962) and Clostridium acetobutylicum (ATCC824) as examples to describe the specific content of the invention in detail , does not limit the selection of four sources of microorganisms in the composite microbial inoculum of the present invention.

[0029] (1) Activate the above Escherichia coli (ATCC11775), Pseudomonas putida (CICC21906), Penicillium decumbens (CGMCC3.7962) and Clostridium acetobutylicum (ATCC824) into their respective slant medium for preservation , Escherichia coli was cultured at 37°C for 16 hours, Pseudomonas putida was cultured at 30°C for 18 hours, Penicillium decumbens was cultured at 28°C for 72 hours, and Clostridium acetobutylicum was cultured at 36°C for 48 hours.

[0030] Preservation of four kinds of microorganisms uses the slant medium formula as follows:

[0031] ...

Embodiment 2

[0040] Example 2 Using a compound microbial agent to treat lignite in an open-pit coal mine in Inner Mongolia to produce methane and humic acid

[0041] 1. The four kinds of microbial fermented liquids prepared in Example 1 are mixed uniformly by the following parts by weight to become a composite microbial bacterial agent: 11 parts by weight of Escherichia coli fermented liquid, 8 parts by weight of Pseudomonas putida fermented liquid, 13 parts by weight of Penicillium decumbens fermented liquid Parts by weight, 7 parts by weight of Clostridium acetobutylicum fermented liquid;

[0042] 2. Select lignite from an open-pit coal mine in Inner Mongolia (sealed quickly after on-site sampling), crush 50kg of pulverized coal through a 30-mesh sieve, divide it into 5 parts on average, add them to 5 15L reactors, and then add cow dung with a mass fraction of 10% For anaerobic fermentation, add corn steep liquor and ammonium sulfate to adjust the carbon-to-nitrogen ratio to 20:1, and ad...

Embodiment 3

[0048] Embodiment 3 Utilizes composite microbial agent to process Gansu lignite to produce methane and humic acid

[0049] 1. The 4 kinds of microorganisms in Example 1 are replaced by the following sources: Escherichia coli (ATCC39075), Penicillium chrysogenum (CICC41988), Pseudomonas putida (ATCC39119), Clostridium acetobutylicum (CICC8018); According to the method of Example 1, four kinds of microbial fermentation liquids were prepared, and mixed uniformly according to the following parts by weight to form a composite microbial bacterial agent I: 17 parts by weight of Escherichia coli fermentation liquid, 11 parts by weight of Pseudomonas putida fermentation liquid, and fermented by Penicillium decumbens 16 parts by weight of liquid, 18 parts by weight of Clostridium acetobutylicum fermentation broth; mix uniformly by the following parts by weight to become composite microbial bacterial agent II: 26 parts by weight of Escherichia coli fermentation broth, 20 parts by weight o...

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Abstract

The invention discloses a method for ex-situ preparation of methane and combined production of humic acid, and a composite microbial agent used in the same, belonging to the field of recycling of brown coal. The composite microbial agent is composed of fermentation broth of four bacteria, i.e., Escherichia coli, Pseudomonas putida, Penicillium decumbens and Clostridium acetobutylicum. The composite microbial agent has strong degradation capability on a variety of celluloses, hydrocarbons, asphaltene and a variety of carbonaceous macro-molecules. As coal is treated by the composite microbial agent, anaerobic digestion time can be shortened, output of methane gas is increased and extraction efficiency of humic acid is improved; moreover, the synergistic effect of the above-mentioned four microbial strains is superior to the effect of any one microbial strain or any two microbial strains.

Description

technical field [0001] The invention belongs to the field of resource utilization of lignite, and relates to a method for non-situ preparation of methane and co-production of humic acid from lignite and a composite bacterial agent used therein. Background technique [0002] my country has extremely rich lignite resources, with proven reserves exceeding 130 billion tons, mainly concentrated in Inner Mongolia, Yunnan, Heilongjiang and other provinces. As a low calorific value coal, lignite generally has a calorific value of only 2,300 to 2,500 calories, a high water content, generally between 32% and 45%, and high volatile matter and ash. The market price of lignite is about 100 yuan per ton. It is neither safe nor economical to transport it abroad. It is generally consumed by local power generation. However, it is currently in the cold winter of the coal market, and the supply of coal production capacity exceeds demand, making its way out even more difficult. [0003] Part o...

Claims

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Application Information

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IPC IPC(8): C12P39/00C12P5/02C08H99/00C07G99/00C12R1/19C12R1/40C12R1/80C12R1/145
CPCY02E50/30
Inventor 李登奎程苏宁支银芳周楚新
Owner 创盛量子科技(温州)有限公司
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