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A kind of rapid propagation method of onion

A kind of onion, fast technology, applied in the field of agriculture, can solve the problems of long growth cycle of onion, low efficiency of tissue culture and rapid multiplication, and difficulty in meeting large-scale production, and achieve rapid breeding, short multiplication time and large multiplication coefficient Effect

Active Publication Date: 2017-10-31
吉林省蔬菜花卉科学研究院
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to solve the problem that the growth period of the shallots is long, and the efficiency of tissue culture and rapid propagation is not high, and it is difficult to meet the large-scale production, and to provide a method for rapid propagation of the shallots

Method used

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  • A kind of rapid propagation method of onion
  • A kind of rapid propagation method of onion
  • A kind of rapid propagation method of onion

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Embodiment 1 Tiller onion stem tip multiplication

[0035] The shoot tip of tillering onion was inoculated in different concentrations of 6-BA, NAA combination and 6-BA, IAA combination medium (Table 1). And NAA in the induction of proliferation, there is a big difference. Among them, the proliferating seedlings induced by the combination of 6-BA and NAA hormones grow better, and have a higher differentiation coefficient. After 4 weeks of culture, the average proliferation coefficient can reach 3.87; although the combination of 6-BA and IAA hormones can also achieve shoot tip Proliferation, but the proliferated seedlings obtained are thin and weak, with low differentiation coefficient, and albino seedlings appear (such as figure 1 ). It can be seen that the effect of IAA is not as good as that of NAA. Therefore, MS+6-BA0.3mg / L+NAA0.1mg / L medium was determined to be the best medium for shoot tip proliferation.

[0036]

[0037] Note: MS is the basic medium.

Embodiment 2

[0038] Embodiment 2 Tiller onion bulb disc multiplication

[0039] Take a tillering onion, remove the corky part of the tillering onion bulb disc, keep the bulb disc with a diameter of 0.4-1 cm centered on the central growth point and the bulb wrapped on the upper part, and obtain the center part of the tillering onion. Rinse the center of the tillering onion with running water for 0.5 h, soak and disinfect with 75% alcohol for 1 min, then soak and disinfect with 1% NaClO solution for 15-20 min, rinse with sterile water for 3-5 times to remove residual NaClO, absorb with filter paper Dry water backup (such as figure 2 ).

[0040] Then remove the upper bulb, keep the bulb disk including the main growth point, cut the bulb disk into 4 parts, remove the main growth point and the main growth point bulb disk, and inoculate them on MS as the basal medium respectively, add 6-BA ( 0.2-0.8 mg / L), NAA (0.1-2. 0mg / L), and cultured in the value-proliferating medium whose pH was adjuste...

Embodiment 3

[0045] Example 3 Test tube balling of tillering onion virus-free seedlings

[0046]Take the tip of the tiller onion, wash it with running water for half an hour, soak it in 75% alcohol for 1 minute, then soak it in 1% NaClO solution for 15-20 minutes, rinse it with sterile water for 3-5 times to remove the residual NaClO, and absorb it with filter paper. Dry the water for backup, peel off the part of the growth point of the tiller onion shoot tip <0.3mm under the microscope, and induce seedlings in the starting medium with MS as the base medium and supplemented with 6-BA and IAA.

[0047] After the stem tips became seedlings, sucrose was used as the basic carbon source to set 5 carbon source sugar concentrations of 30mg / L, 45mg / L, 60mg / L, 75mg / L, and 90mg / L, to explore the effects of different sugar concentrations on the tillering onion test-tube plantlets. For the effect of bulbing, the sugar concentration in the best bulbing medium was screened, and it was found through cult...

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Abstract

The invention discloses a rapid propagation method of onion, which comprises cleaning and sterilizing the central part of the onion. After sterilization of the explants, the upper scales were cut transversely at a distance of 3-5 mm from the bottom end of the central part, and the upper scales were removed along the vertical tangent direction of the main growth point. Through the optimization of the above-mentioned culture process, a rapid propagation system of onion bulb plate was established, and a large number of test-tube seedlings of onion were obtained. The system has a short proliferation time, a large proliferation coefficient and high genetic stability. , through this system, the rapid breeding of onion can be realized, and the planting of the onion industry can be realized.

Description

technical field [0001] The invention belongs to the technical field of agriculture, and in particular relates to a method for rapid propagation of shallots. Background technique [0002] Plant tissue culture technology is a new technology developed on the basis of plant physiology. It refers to the cultivation of isolated plant organs, tissues, cells, and protoplasts on artificially prepared medium under sterile conditions. Conditions for cultivation, induction of callus or adventitious buds, etc., or the process of growing into a complete plant. Since the establishment of this technology in the early 20th century, it has been continuously developed in theoretical research and applied technology. The huge economic and social benefits have had a profound impact on the fields of modern agriculture and medicine. [0003] Allium scallion, also known as cold onion, is a plant of the genus Allium in the family Liliaceae, a perennial herb. In recent years, many scholars at home ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 张悦程英魁王健鹂王秀峰马艺荞刘井莉娄琦冯博
Owner 吉林省蔬菜花卉科学研究院
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