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Tissue culture and rapid propagation method for ajuga reptans

A technology of tissue culture and herba sinensis, which is applied in horticultural methods, botany equipment and methods, horticulture, etc., can solve the problems of restricted application and low cutting propagation coefficient, and achieve the goal of reducing time cost, reducing preparation difficulty, and shortening multiplication time Effect

Inactive Publication Date: 2017-11-28
ZHEJIANG XIAOSHAN COTTON & FLAX RES INST
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  • Description
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  • Application Information

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Problems solved by technology

However, Creeping Sclerosus can not be propagated by seeds, and the propagation coefficient of cuttings is extremely low, which seriously restricts its application in the garden market.

Method used

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  • Tissue culture and rapid propagation method for ajuga reptans
  • Tissue culture and rapid propagation method for ajuga reptans
  • Tissue culture and rapid propagation method for ajuga reptans

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Embodiment 1

[0022] A. Selection and pretreatment of explants: choose the vigorous growth and no pests and diseases of Creeping Grass 'Burgundy Light' as the mother plant for drawing materials, such as figure 1 . Two days before sampling, use 80% mancozeb or 10% thiophanate-methyl 1000-1500 times solution to spray the sampled mother plant in all directions, so that the mother plant will carry as little endophytic bacteria as possible in subsequent operations , to ensure the disinfection effect of explants. When sampling, cut off the young tender stems at the top, remove the leaves, and keep only the stems, and wash them in tap water for half an hour. This pretreatment can reduce the pollution rate by about 50%, and the effect is very good.

[0023] B. Disinfection of explants: put the above-mentioned cleaned explants in 70-75% alcohol, shake for 30 seconds, pour off the alcohol immediately, and wash it once with sterilized water, then place the explants in 0.1 % mercuric acid solution,...

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Abstract

The invention discloses a tissue culture and rapid propagation method for ajuga reptans. The method comprises the steps of (A) selecting explants, namely adopting a plant which healthy grows and is free of diseases and pests as a stock plant, chipping a top-end young stem as an explant; (B) carrying out explant treatment, namely carrying out disinfection treatment on the explant by adopting alcohol and a mercury chloride solution successively; (C) carrying out proliferation, namely selecting two relatively economical hormones, namely 6-benzyladenine (6-BA) and naphthylacetic acid (NAA) for inducible proliferation on the explant; and (D) rooting and transplanting, namely stripping proliferated buds to a rooting medium, decapping and hardening after the roots develop well, cleaning the medium and planting in a mixed medium of peat and perlite. According to the method, the operation is simple and raw materials are economical and practical, and a lot of tissue culture seedlings can be obtained within a short period of time for landscaping application.

Description

technical field [0001] The invention belongs to the technical field of tissue culture and rapid propagation of ornamental perennial root cover plants. Background technique [0002] Creeping sinensis is a new type of perennial ground cover flower native to Europe and America and introduced into my country from Europe and America. Creeping Muscaria sinensis and Muscaria sinensis native to China and long-term used as Chinese herbal medicines ( Ajuga ciliata Bunge.) are fundamentally different. Creeping Musculus is short in plant shape, evergreen in four seasons, with three seasons of colorful leaves, and a scape of about 30 cm. It is suitable for flower mirrors, ground covers, flower beds, and potted flowers. The flowers and leaves are beautiful and the viewing effect is excellent. However, S. creeper cannot be propagated by seeds, and the propagation coefficient of cuttings is extremely low, which seriously restricts its application in the garden market. There is an urgen...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A61L2/18
CPCA01H4/001A01H4/008A61L2/18
Inventor 马广莹邹清成史小华金亮安霞朱开元
Owner ZHEJIANG XIAOSHAN COTTON & FLAX RES INST
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