Method for quickly and efficiently germinating garlic aerial bulbs
A technology for aerial bulbs and garlic is applied in the field of rapid and efficient germination of aerial bulbs of garlic, which can solve the problems of limiting the large-scale application of the aerial bulb reproduction technology of garlic, loss of scientific research and production, and low reproduction coefficient, and achieves shortening germination time, The effect of shortened germination time and high germination rate
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Embodiment 1
[0046] The garlic varieties harvested in the current year are 'Cangshan Dahongpi' (early maturity), 'Sichuan Ershuizao' (medium early maturity), 'Sichuan Soft Leaf Garlic' (medium maturity), 'Majiang Red Garlic' (late maturity) Aerial bulbs were used as test materials. Choose healthy and plump aerial bulbs with typical characteristics of varieties, and divide them into three grades: large, medium and small according to the diameter of the aerial bulbs. Soak in 0.3% carbendazim and 0.3% potassium dihydrogen phosphate mixed solution for 12 hours, then soak in sterile distilled water for 12 hours, sow in vermiculite with water content of 60%, 20 grains per plate, repeat 5 times. The standard for germination is the length of the germ to the length of the aerial bulb. Count the number of germinated seeds every day, and calculate the five germination indicators of germination potential, germination rate, germination index, vitality index and average germination time. The calculatio...
Embodiment 2
[0058] Aerial bulbs of garlic varieties 'Cangshan Dahongpi', 'Sichuan Ershuizao', 'Sichuan Soft Leaf Garlic' and 'Majiang Red Garlic' harvested in the year, healthy and plump, of the same size and with typical traits were selected as test materials. , each kind of aerial bulb diameter size is the optimal diameter range that embodiment 1 screens. Aerial bulbs were soaked in a mixed solution of 0.3% carbendazim and 0.3% potassium dihydrogen phosphate for 12 hours, then soaked in sterile distilled water for 12 hours, and sowed in vermiculite with a water content of 60%, 20 grains per plate, 5 Repeatedly, randomly placed in the light incubator. Culture under 20 dark conditions, 20 light (12h, 10000Lux) culture as control. Take the length of the germ to the length of the aerial bulb as the germination standard, and the measurement index is the same as in Example 1.
[0059] Table 2 Effects of different light conditions on germination indicators of garlic aerial bulbs
[0060] ...
Embodiment 3
[0063] The aerial bulb material (same as Example 2) was stored in 4 refrigerators, and the storage time was respectively 0, 7, 14, 21, and 28 days, and the aerial bulb germination test was carried out after storage. Soak in 0.3% carbendazim and 0.3% potassium dihydrogen phosphate mixed solution for 12 hours, then soak in sterile distilled water for 12 hours, sow in vermiculite with water content of 60%, 20 grains per plate, repeat 5 times, Randomly placed in the light incubator. Cultured at 20 dark conditions, 25 dark culture as a control. Take the length of the germ to the length of the aerial bulb as the germination standard, and the measurement index is the same as in Example 1.
[0064] Table 3 Effects of combination of storage time and germination temperature on germination index of aerial bulbs of ‘Cangshan Dahongpi’
[0065]
[0066] The results are shown in Table 3. The aerial bulbs of 'Cangshan Dahongpi' were stored for 28 days and then cultured at 25°C. The germ...
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