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A method for obtaining wild explants of eosinopsis

An acquisition method and explant technology, which is applied in the field of acquisition of wild explants of Echinopsis euphratica, can solve problems such as patents and literatures that have not been reported, and achieve the effects of increasing tolerance, reducing the rate of bacteria transmission, and reducing damage

Inactive Publication Date: 2016-04-27
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The present invention greatly improves the effective acquisition rate of wild eelgrass wild explants by pre-cultivating wild eelgrass cloned seedlings and applying an effective surface disinfection method. At present, no relevant patents and documents using this technical solution have been reported.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Example 1: A method for obtaining explants of shoot tip explants of Z. echinophyllum, the implementation steps comprising:

[0015] (1) Field collection of cloned fragments: Collect eelgrass plants in sea areas during high tide and low tide, dig out eelgrass and rhizomes from the bottom mud with a shovel, and clean the bottom mud on the rhizomes in situ, and the The cloned fragments of the cloned seedlings were packed into plastic ziplocks and brought back to the laboratory under refrigeration;

[0016] (2) Indoor temporary cultivation of cloned seedlings: break off the seedlings on the cloned fragments from the connection with the main branch, and clean up the leaves and leaf sheaths and some old roots that have begun to fall off and rot at the ends of the seedlings, and put them in groups of 5. Put it into a 1000ml beaker filled with natural seawater and cultivate it in a light incubator. The minimum temperature of the incubator can only be set to 10°C), and it wil...

Embodiment 2

[0021] Example 2: A method for obtaining eel leaf stem explants, the steps of which are:

[0022] (1) Field collection of cloned fragments: Collect eelgrass plants in sea areas during high tide and low tide, dig out eelgrass and rhizomes from the bottom mud with a shovel, and clean the bottom mud on the rhizomes in situ, and the The cloned fragments of the cloned seedlings were packed into plastic ziplocks and brought back to the laboratory under refrigeration;

[0023] (2) Indoor temporary cultivation of cloned seedlings: break off the seedlings on the cloned fragments from the connection with the main branch, and clean up the leaves and leaf sheaths and some old roots that have begun to fall off and rot at the ends of the seedlings, and put them in groups of 5. Put it into a 1000ml beaker filled with natural seawater and cultivate it in a light incubator. The minimum temperature of the incubator can only be set to 10°C), and it will rise or fall by 1°C every day in the fu...

Embodiment 3

[0028] Example 3: A method for obtaining eosinophila root explants, the steps of which are:

[0029] (1) Field collection of cloned fragments: Collect eelgrass plants in sea areas during high tide and low tide, dig out eelgrass and rhizomes from the bottom mud with a shovel, and clean the bottom mud on the rhizomes in situ, and the The cloned fragments of the cloned seedlings were packed into plastic ziplocks and brought back to the laboratory under refrigeration;

[0030] (2) Indoor temporary cultivation of cloned seedlings: break off the seedlings on the cloned fragments from the connection with the main branch, and clean up the leaves and leaf sheaths and some old roots that have begun to fall off and rot at the ends of the seedlings, and put them in groups of 5. Put it into a 1000ml beaker filled with natural seawater and cultivate it in a light incubator. The minimum temperature of the incubator can only be set to 10°C), and it will rise or fall by 1°C every day in the...

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Abstract

The present invention relates to a method for obtaining wild explants of eelgrass, which comprises the following steps: digging out the plants in sea areas where eelweed grows vigorously; Fold off the branches, put them into natural seawater, and temporarily raise them in the light incubator. After 3-5 days, replace the natural seawater with sterilized natural seawater to continue the cultivation; after 5 days, select the rhizomes containing more than 5 seedlings, Trim on an ultra-clean workbench; wash the pruned seedlings with sterilized seawater 3-5 times, then soak them in 10% hydrogen peroxide solution for 15 minutes, and wash them with sterilized seawater for 5 times; The overgrown seedlings are then pruned again to obtain explants of interest, such as root tips, shoot tips or rhizomes, for inoculation. The invention guarantees the low pollution rate and low lethality rate in primary culture when wild plants are used as explant donors in the tissue culture of the zinnia, and provides the most basic technical support for the success of the tissue culture of the zinnia.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and relates to a method for obtaining wild explants of Echinopsis. Background technique [0002] Eelweed ( Zostera marina L.) is a representative species of 72 species of seagrass in the world, widely distributed in the temperate seas of the northern hemisphere, and also distributed in the coastal waters of Shandong, Hebei and Liaoning in my country. Although the tissue culture technology of many terrestrial plants is mature, the tissue culture technology of seagrass, which is a monocotyledonous plant, has not made a major breakthrough after more than 40 years of research. One of the important reasons is that there is no suitable wild explant method of obtaining . [0003] Although sterile seedlings are considered to be the best explant choice for tissue culture of Z. Time is limited and the germination rate of seeds is also uncertain. Therefore, it is necessary to seek methods fo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 郑凤英李乐乐陈玉金艳梅韩晓弟刘雪芹窦国栋
Owner SHANDONG UNIV
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