Key technology of organic additive for roxburgh anoectochilus terminal bud industrialization intermediate propagation

Abstract

The invention provides a key technology of an organic additive for roxburgh anoectochilus terminal bud industrialization intermediate propagation, belonging to a massive propagation and cultivation technology for the roxburgh anoectochilus terminal bud. According to the key technology, a terminal bud and a stem of the roxburgh anoectochilus terminal bud are taken as the material, in industrialization, the organic additive is adjusted and added timely, and the entire plant grows up after induction cultivation, enrichment cultivation and root induction, and the rooting percentage can reach 99%; and after hardening-seedling, the plant can be transplanted into humus, and the rate of survival can reach above 99%. According to the technology, the time for enrichment cultivation is short, the expanding propagation coefficient is high, the seedling can grow up once and is healthy, the whole roxburgh anoectochilus terminal bud plant is thick, the stem is thick and the roots are flourishing, the color of leaves is dark, the leaves have obvious fine hair, the leaf vein gold threads are clear, the rate of survival is high, the weight of each plant is 4.5-5.5g, and the economic efficiency is obvious.

Description

technical field [0001] The invention belongs to the industrialized rapid propagation and cultivation technology of clematis. Background technique [0002] Clematis ( Shorthairy Antenoron ) belongs to Orchidaceae orchids [ Anoectochilus roxburghii (wall.) Lindl ] A perennial rare Chinese herbal medicinal plant, its Chinese name is Huayekailianlan, and it is known as "King of Medicine", "Golden Grass", "Miraculous Medicine", "Black Ginseng" and so on. Also known as golden thread, golden earring, black ginseng, golden thread tiger head banana, golden thread into the bone, money grass, golden thread stone pine, golden stone silkworm, juvenile red, small leaf golden earring, hemp leaf vegetable. Mainly distributed in China, Japan, Sri Lanka, India, Nepal and other countries, it grows in dense forests in deep mountains or in moist grass by streams or under forests, especially in humid areas under broad-leaved forests. Scattered, rarely densely grown in patches, Aurantia clemati...

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a tissue culture method for industrialized rapid propagation of Clematis clematis, provide excellent purified tissue culture seedlings for large-scale planting, and solve the problem of tiny seeds and incomplete embryo development in the prior art, which are extremely difficult to produce under natural conditions. For problems such as low germination and reproduction coefficients, choosing a suitable medium for propagation with organic additives will have a direct impact on the product quality and image of finished seedlings of A. , healthy seedlings, thick stems, well-developed root system, thick leaves with obvious flannelette shape, clear gold lines in veins, high survival rate, and a weight of 4.5-5.5 grams per plant, which has significant economic benefits. Further breakthroughs have been made in the key technologies of industrialized and industrialized production, which is of great significance for the promotion and development of clematis seed resources and its application in plant genetic engineering and the establishment and improvement of genetic transformation systems

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