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Rapid propagation method for novel apple varieties Ruiyang and Ruixue

A new variety, apple technology, applied in the field of rapid propagation of new apple varieties Ruiyang and Ruixue, can solve the problems of aggravation and affecting the growth of fruit trees, and achieve the effect of favorable preservation, promotion of further development, efficient and convenient breeding technology

Active Publication Date: 2020-01-07
NORTHWEST A & F UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Traditional fruit tree seedling propagation such as direct large-scale budding can solve the problem of fruit tree seedling propagation to a certain extent, but the probability of seedling infection by viruses will be greatly increased, seriously affecting the growth of fruit trees.

Method used

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  • Rapid propagation method for novel apple varieties Ruiyang and Ruixue
  • Rapid propagation method for novel apple varieties Ruiyang and Ruixue
  • Rapid propagation method for novel apple varieties Ruiyang and Ruixue

Examples

Experimental program
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Effect test

Embodiment 1

[0043] The method for rapid propagation of a new apple variety Ruiyang provided by the embodiment of the present invention includes the following steps:

[0044] The first step is to use the annual branches of the new apple variety Ruiyang healthy mother tree as the material. After the dormant branches are stored in winter sand, they are taken out in the following spring and soaked in 1% NaClO solution for 10 minutes. Change the water and cut off the old cuts every day. Seal other cuts on the branches with parafilm. When the buds on the hydroponic branches are about 1.5 cm in length, remove the stem tips and remove the unfolded leaves. Rinse with tap water for 30 minutes, treat with 75% alcohol for 5-8s, 0.1% HgCl 2 Treat for 8 minutes, rinse with sterile water 5-7 times for later use;

[0045] In the second step, use absorbent paper to absorb the surface moisture of the treated stem tip and inoculate it to a content of 2.0 mg·L -1 Carry out dark culture in MS medium for 3 days, ad...

Embodiment 2

[0060] The method for rapid propagation of a new apple variety Ruixue provided by the embodiment of the present invention includes the following steps:

[0061] The first step is to use the annual branches of the new apple variety Ruixue healthy mother tree as the material. After the dormant branches are stored in winter sand, they are taken out in the following spring and soaked in 1% NaClO solution for 10 minutes. ), change the water every day and cut off the old cuts, and seal the other cuts on the branches with parafilm. When the buds on the hydroponic branches are about 1.5 cm in length, remove the stem tips and remove the unfolded leaves. Tap water Rinse for 30 minutes, treat with 75% alcohol for 5-8s, 0.1% HgCl 2 Treat for 8 minutes, rinse with sterile water 5-7 times for later use;

[0062] In the second step, use absorbent paper to absorb the surface moisture of the treated stem tip and inoculate it to a content of 2.0 mg·L -1 Carry out dark culture in MS medium for 3 days...

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Abstract

The invention belongs to the technical field of fruit tree seedling cultivation, and discloses a rapid propagation method for novel apple varieties Ruiyang and Ruixue; a culture medium is adopted forMS+PVP 2.0 g.L primary culture; another culture medium is adopted for MS+6-BA 0.5 mg.L<-1> to 0.75 mg.L<-1>+IBA 0.02 mg.L<-1> to 0.05 mg.L<-1> subculture treatment; a rooting medium is adopted for 1 / 2MS+IBA 0.75 mg<-1> to 1.1 mg.L<-1> rooting culture; rapid propagation of apple varieties is achieved by hardening and transplanting seedlings with seedling substrate moss. The method disclosed by theinvention has important significance for producing high-quality late-maturing upgraded apple seedlings.

Description

Technical field [0001] The invention belongs to the technical field of fruit tree seedling cultivation, and in particular relates to a method for rapid propagation of new apple varieties Ruiyang and Ruixue. Background technique [0002] The new apple varieties Ruiyang and Ruixue were led by Professor Zhao Zhengyang, a professor at Northwest A&F University and the chief expert on apples in Shaanxi Province. The team has worked hard for more than 20 years and achieved an important breakthrough in the selection and breeding of new apple varieties. These new high-quality late-ripening apple varieties have all passed the national forestry improved varieties certification on March 29, 2019. Among them, Ruiyang is a high-quality, high-yield, late-maturing, and red variety, which was bred from the cross of Fuji and Qin Guan; Ruixue is a high-quality, late-maturing, and yellow variety, which was bred by the cross of Qinfu No. 1 and Pink Lady. to make. Several years after the breeding of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01G31/00A01G24/22
CPCA01H4/008A01H4/001A01G31/00A01G24/22
Inventor 刘莉冯瑞芳王英王元基刘聪孙鲁龙杨亚州赵政阳
Owner NORTHWEST A & F UNIV
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