Method for culturing in vitro embryos and regenerating plants of ormosia hosiei in western Hubei province
A technology for culturing red beans and embryos, which is applied in the tissue culture field of preservation and mass propagation technology, can solve the problems of poor water permeability of the seed coat, late fruiting age, hard seeds, etc., and achieve short multiplication time, high multiplication coefficient, and survival rate high effect
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[0029] 1) Method of obtaining materials: The seeds of the red bean tree in western Hubei with full grains are selected as the material, and they are wrapped in a damp cloth after picking, and rinsed with detergent after being brought back to the laboratory, and then placed in running water to drip;
[0030] 2) Preparation of culture medium: Prepare each culture medium according to the following formula:
[0031] Embryo induction medium: WPM+BA (1.0mg·L -1 ) + KT (0.5mg·L -1 )+NAA (0.1mg·L -1 )+Agar (6.0g·L -1 ) +Su (20g·L -1 )+Activated carbon (1.0~1.5 g·L -1 );
[0032] Bud propagation medium: WPM+BA (1.5mg·L -1 ) + KT (1.0mg·L -1 ) + TDZ(0.2mg·L -1 ) + NAA (0.3mg·L -1 ) + Agar (6.0g·L -1 ) +Su (20g·L -1 ) + Activated carbon (1.0~1.5 g·L -1 );
[0033] Strong seedling culture medium: WPM+BA (1.5mg·L -1 ) + KT (1.0mg·L -1 ) + TDZ(0.2mg·L -1 ) + NAA (0.5mg·L -1 ) + Agar (6.0g·L -1 ) +Su (30g·L -1 ) + Activated carbon (1.5~2.0 g·L -1 );
[0034] Rooting medium: 1 / 2 WPM + NAA (0.3mg·L ...
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