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Rapid propagation method of allium victorialis

A kind of onion and fast technology, applied in the field of agriculture, can solve the problems of low tissue culture and rapid multiplication efficiency, long growth cycle of onion, and difficulty in meeting large-scale production, and achieve rapid breeding, short multiplication time, and large multiplication coefficient Effect

Active Publication Date: 2016-01-27
吉林省蔬菜花卉科学研究院
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to solve the problem that the growth period of the shallots is long, and the efficiency of tissue culture and rapid propagation is not high, and it is difficult to meet the large-scale production, and to provide a method for rapid propagation of the shallots

Method used

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  • Rapid propagation method of allium victorialis
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  • Rapid propagation method of allium victorialis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Embodiment 1 Tiller onion stem tip multiplication

[0035] The shoot tip of tillering onion was inoculated in different concentrations of 6-BA, NAA combination and 6-BA, IAA combination medium (Table 1). And NAA in the induction of proliferation, there is a big difference. Among them, the proliferating seedlings induced by the combination of 6-BA and NAA hormones grow better, and have a higher differentiation coefficient. After 4 weeks of culture, the average proliferation coefficient can reach 3.87; although the combination of 6-BA and IAA hormones can also achieve shoot tip Proliferation, but the proliferated seedlings obtained are thin and weak, with low differentiation coefficient, and albino seedlings appear (such as figure 1 ). It can be seen that the effect of IAA is not as good as that of NAA. Therefore, MS+6-BA0.3mg / L+NAA0.1mg / L medium was determined to be the best medium for shoot tip proliferation.

[0036]

[0037] Note: MS is the basic medium.

Embodiment 2

[0038] Embodiment 2 Tiller onion bulb disc multiplication

[0039] Take a tillering onion, remove the corky part of the tillering onion bulb disc, keep the bulb disc with a diameter of 0.4-1 cm centered on the central growth point and the bulb wrapped on the upper part, and obtain the center part of the tillering onion. Rinse the center of the tillering onion with running water for 0.5h, soak and disinfect with 75% alcohol for 1min, then soak and disinfect with 1% NaClO solution for 15-20min, rinse with sterile water 3-5 times to remove residual NaClO, and blot dry with filter paper water backup (eg figure 2 ).

[0040] Then remove the upper bulb, keep the bulb disk including the main growth point, cut the bulb disk into 4 parts, remove the main growth point and the main growth point bulb disk, and inoculate them on MS as the basal medium respectively, add 6-BA ( 0.2-0.8mg / L), NAA (0.1-2.0mg / L), PH adjusted to 5.8 value-added culture medium to induce clustered buds. Under ...

Embodiment 3

[0045] Example 3 Tiller onion virus-free seedling test tube balling

[0046]Take the tip of the tiller onion, wash it with running water for half an hour, soak it in 75% alcohol for 1 minute, then soak it in 1% NaClO solution for 15-20 minutes, rinse it with sterile water for 3-5 times to remove the residual NaClO, and absorb it with filter paper. Dry the water for backup, peel off the part of the growth point of the tiller onion shoot tip <0.3mm under the microscope, and induce seedlings in the starting medium with MS as the base medium and supplemented with 6-BA and IAA.

[0047] After the stem tips became seedlings, sucrose was used as the basic carbon source to set 5 carbon source sugar concentrations of 30mg / L, 45mg / L, 60mg / L, 75mg / L, and 90mg / L, to explore the effects of different sugar concentrations on the tillering onion test-tube plantlets. For the effect of bulbing, the sugar concentration in the best bulbing medium was screened, and it was found through cultivation...

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Abstract

The invention discloses a rapid propagation method of allium victorialis. The rapid propagation method of the allium victorialis comprises the following steps: washing and disinfecting the central part of the allium victorialis; carrying out explant sterilization, then transversely cutting from a position with a distance of 3-5mm from the bottom end in the central part and removing upper scales, longitudinally cutting into four parts along a vertical tangent direction of a main growth point, removing the main growth point and a bulb pan where the main growth point is located, inducing clustered shoots and culturing the clustered shoots into seedlings. A rapid propagation system of the bulb pan of the allium victorialis is built by optimizing the abovementioned culture process, and a large number of allium victorialis test tube seedlings are obtained. The system is short in proliferation time, high in proliferation coefficient and high in hereditary stability; through the system, the rapid propagation of the allium victorialis can be achieved, and the planting of the allium victorialis industry can be achieved.

Description

technical field [0001] The invention belongs to the technical field of agriculture, and in particular relates to a method for rapid propagation of shallots. Background technique [0002] Plant tissue culture technology is a new technology developed on the basis of plant physiology. It refers to the cultivation of isolated plant organs, tissues, cells, and protoplasts on artificially prepared medium under sterile conditions. Conditions for cultivation, induction of callus or adventitious buds, etc., or the process of growing into a complete plant. Since the establishment of this technology in the early 20th century, it has been continuously developed in theoretical research and applied technology. The huge economic and social benefits have had a profound impact on the fields of modern agriculture and medicine. [0003] Allium scallion, also known as cold onion, is a plant of the genus Allium in the family Liliaceae, a perennial herb. In recent years, many scholars at home ...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 张悦程英魁王健鹂王秀峰马艺荞刘井莉娄琦冯博
Owner 吉林省蔬菜花卉科学研究院
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