Method for error correction in transgenic organism quantitative determination
A quantitative detection and transgenic technology, applied in biochemical equipment and methods, microbial determination/inspection, etc., can solve the problems of different nucleic acid volumes, differences, biases, etc., to achieve high sensitivity, ensure accuracy and accuracy , Eliminate the effect of difference interference data analysis
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[0070] Quantitative detection of components in rice powder
[0071] 1. Materials and methods
[0072] 1. The sample to be tested is the standard substance of rice seed powder of transgenic rice Kechidao No. 2 with a mass fraction of 2% (developed by China Institute of Metrology)
[0073] 2. Accurately weigh the sample to be tested and the quality control product (the standard substance of the transgenic rice Kemodao No. 2 rice seed powder with a mass fraction of 1% and 5% content) with a balance
[0074] 3. Using the Plant Genome Extraction Kit GenomicDNAPurificationKit (Promega, USA) extracts the genomic DNA of the sample to be tested and the quality control product, and uses a UV spectrophotometer and a Picogreen kit to determine the purity and concentration of the extracted DNA, respectively.
[0075] 4. The standard (containing about 10 6 、10 5 、10 4 、10 3 、10 2 Copy / ml Plasmid Molecular Standard Substances of Kechidao 2 and RBE4 Gene Fragment) and the DNA of the s...
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