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Method for rapidly detecting phenyllactic acid content by using reversed-phase high performance liquid chromatography

A technology of reversed-phase high performance liquid phase and phenyllactic acid, which is applied in the field of high performance liquid chromatography analysis, can solve the problems of high labor and preparation costs, shortened detection time, long detection time, etc., and achieves accurate and reliable detection results, mobile phase and instrumentation. The effect of low requirements and fast peak time

Inactive Publication Date: 2019-05-21
SHANGHAI OCEAN UNIV
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  • Application Information

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Problems solved by technology

[0005] Aiming at the problems of high instrument requirements, long detection time, high labor and preparation costs and low sensitivity when detecting phenyllactic acid at present, the purpose of the present invention is to provide a method for quickly detecting the content of phenyllactic acid by reversed-phase high-performance liquid chromatography, High accuracy, good stability, simple operation, low requirements on mobile phase and instruments, cost saving, fast peak time, shortened detection time in the production process, and accurate and reliable detection results

Method used

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  • Method for rapidly detecting phenyllactic acid content by using reversed-phase high performance liquid chromatography
  • Method for rapidly detecting phenyllactic acid content by using reversed-phase high performance liquid chromatography
  • Method for rapidly detecting phenyllactic acid content by using reversed-phase high performance liquid chromatography

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Effect test

Embodiment 1

[0033] Utilize the method for rapid detection of phenyllactic acid content by reversed-phase high-performance liquid chromatography, the specific steps are:

[0034] Step a, configuring the mobile phase, filtering, degassing, adjusting the instrument, and balancing the chromatographic column;

[0035] Step b. Use the standard solution of phenyllactic acid prepared by Shanghai Ziqi Biotechnology Co., Ltd. to prepare the standard solution of phenyllactic acid, and dilute in sequence to obtain a concentration of 10.0 μg / mL, 5.0 μg / mL, 2.0 μg / mL, 1.0 μg / mL, and 0.5 μg / mL standard solution, using high performance liquid chromatography to carry out high performance liquid phase analysis on phenyllactic acid standard solution, the standard solution of each concentration was injected twice, using Agilent1260 off-line data processing software for data processing, the chromatogram is as follows figure 1 As shown, the retention time in the figure is the peak of phenyllactic acid at 6.25...

Embodiment 2

[0040] In order to verify the feasibility and accuracy of the above-mentioned embodiment 1, a precision test was carried out. The test used a standard solution of phenyllactic acid with a concentration of 2.0 μg / mL, and continuously injected 8 times. Using the same chromatographic conditions as in the embodiment, the measurement results Table 2 shows.

[0041] Table 2: Precision test values

[0042] serial number

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Abstract

The invention discloses a method for rapidly detecting a phenyllactic acid content by using reversed-phase high performance liquid chromatography and belongs to the food detection technology field. Chromatographic conditions comprise that a C18 chromatographic column (4.6*250 mm, 5mum) is included; a mobile phase A is a 0.05% trifluoroacetic acid water solution; a mobile phase B is acetonitrile, and the volume ratio of mobile phases A and B is 3:1; a flow rate is 1 mL / min; a sample size is 10 muL; a chromatographic column temperature is 20-30 DEG C; and a detection wavelength is 210 nm. Impurities and phenyllactic acid in a sample to be tested are separated, the content of the phenyllactic acid is detected, and qualitative and quantitative operation is performed on the phenyllactic acid through retention time and a peak area. The operation of the method is simple, detection is rapid, cost is low, qualitative and quantitative operation is accurate, separation with an impurity peak can be achieved, and the method is suitable for the rapid batch detection of a total phenyllactic acid amount in food and a fermentation solution.

Description

technical field [0001] The invention belongs to the technical field of high-performance liquid chromatography analysis, and relates to the rapid detection of phenyllactic acid content in food and fermented liquid, in particular to a method for rapidly detecting the content of phenyllactic acid by reverse-phase high-performance liquid chromatography. Background technique [0002] Phenyllactic acid is a new type of antibacterial substance produced by lactic acid bacteria. It has broad-spectrum antibacterial properties and can inhibit the growth of a variety of food-borne pathogens and spoilage bacteria, such as Listeria monocytogenes, Escherichia coli, Staphylococcus aureus and Salmonella, etc. Phenyllactic acid occurs naturally in honey, yeast dough, and Chinese pickles. [0003] At present, the detection of phenyllactic acid mainly includes thin-layer chromatography and high-performance liquid chromatography. The detection limit of thin-layer chromatography is high and the ...

Claims

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Application Information

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IPC IPC(8): G01N30/89
Inventor 欧杰张雯李柏林谢佳雨
Owner SHANGHAI OCEAN UNIV
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